1. Performance of peripheral (serum and molecular) blood markers for diagnosis of endometriosis
- Author
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Luisa Valvassori, Giuseppe Mignemi, Mohamed Mabrouk, Stefano Venturoli, Elisabetta Caramelli, Paola De Sanctis, Giulia Montanari, Manuela Guerrini, Amira Elmakky, Antonio Farina, Cinzia Zucchini, Gioia Villa, Linda Manuzzi, Renato Seracchioli, Mabrouk M., Elmakky A., Caramelli E., Farina A., Mignemi G., Venturoli S., Villa G., Guerrini M., Manuzzi L., Montanari G., De Sanctis P., Valvassori L., Zucchini C., and Seracchioli R.
- Subjects
Adult ,Vascular Endothelial Growth Factor A ,Pathology ,medicine.medical_specialty ,CA-19-9 Antigen ,endocrine system diseases ,Survivin ,Endometriosis ,Matrix metalloproteinase ,Inhibitor of Apoptosis Proteins ,medicine ,Humans ,Blood markers ,Endometriosi ,CA 125 and CA 19-9 ,MMP ,business.industry ,Obstetrics and Gynecology ,General Medicine ,medicine.disease ,Human genetics ,Peripheral ,Matrix Metalloproteinase 9 ,ROC Curve ,Mrna level ,CA-125 Antigen ,Case-Control Studies ,Peripheral blood marker ,Ovarian Endometriosis ,Female ,Matrix Metalloproteinase 3 ,business ,Biomarkers - Abstract
PURPOSE: To quantify the mRNA levels of MMP-3, MMP-9, VEGF and Survivin in peripheral blood and the serum levels of CA-125 and Ca19-9 in women with and without endometriosis and to investigate the performance of these markers to differentiate between deep and ovarian endometriosis. METHODS: A case control study enrolled a series of 60 patients. Twenty controls have been matched with 20 cases of ovarian and 20 cases of deep endometriosis. Univariable and multivariable performance of serum CA125 and CA19-9, mRNA for Survivin, MMP9, MMP3 and VEGF genes have been evaluated by means of ROC curves and logistic regression, respectively. RESULTS: No difference in markers' concentration was detected between ovarian and deep endometriosis. In comparison with controls, serum CA125 and CA19 yielded the better sensitivity followed by mRNA for Survivin gene (81.5, 51.9 and 7.5% at 10% false positive rate, respectively). Multivariable estimated odds of endometriosis yielded a sensitivity of 87% at the same false positive rate. CONCLUSIONS: A combination of serum and molecular markers could allow a better diagnosis of endometriosis.
- Published
- 2011
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