1. STRA8-RB interaction is required for timely entry of meiosis in mouse female germ cells.
- Author
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Shimada R, Kato Y, Takeda N, Fujimura S, Yasunaga KI, Usuki S, Niwa H, Araki K, and Ishiguro KI
- Subjects
- Animals, Female, Male, Mice, Gene Expression Regulation, Germ Cells metabolism, Sexual Maturation, Retinoblastoma Protein, Adaptor Proteins, Signal Transducing metabolism, Meiosis
- Abstract
Meiosis is differently regulated in males and females. In females, germ cells initiate meiosis within a limited time period in the fetal ovary and undergo a prolonged meiotic arrest until puberty. However, how meiosis initiation is coordinated with the cell cycle to coincide with S phase remains elusive. Here, we demonstrate that STRA8 binds to RB via the LXCXE motif. Mutation of the RB-binding site of STRA8 in female mice delays meiotic entry, which consequently delays progression of meiotic prophase and leads to precocious depletion of the oocyte pool. Single-cell RNA-sequencing analysis reveals that the STRA8-RB interaction is required for S phase entry and meiotic gene activation, ensuring precise timing of meiosis initiation in oocytes. Strikingly, the results suggest STRA8 could sequester RB from E2F during pre-meiotic G1/S transition. This study highlights the gene regulatory mechanisms underlying the female-specific mode of meiotic initiation in mice., (© 2023. Springer Nature Limited.)
- Published
- 2023
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