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Industri minyak kayu putih di Indonesia yang dominan berbahan baku daun tanaman Melaleuca cajuputi secara umum masih memerlukan perbaikan kinerja antara lain kinerja proses penyulingan untuk meningkatkan rendemen minyak kayu putih yang dihasilkan. Tujuan kegiatan pengabdian pada masyarakat ini adalah untuk melakukan pendampingan untuk meningkatkan pengetahuan mitra dalam untuk meningkatkan kinerja proses penyulingan minyak kayu putih minyak kayu putih yang dilakukan. Tahapan kegiatan terdiri dua tahapan sebagai yaitu (1) pengamatan terhadap keseluruhan proses penyulingan minyak kayu putih mulai dari bahan baku yang digunakan daun kayu putih, perlakuan pendahuluan bahan baku sebelum dilakukan proses penyulingan, dan proses penyulingan yang dilakukan, dan (2) penyuluhan dan pendampingan terhadap mitra tentang usul upaya peningkatan kinerja proses penyulingan minyak kayu putih. Berdasarkan hasil pengabdian pada masyarakat yang telah dilakukan maka dapat disimpulkan bahwa untuk meningkatkan kinerja proses penyulingan minyak kayu putih memerlukan perlakuan pendahuluan terhadap daun kayu putih yang digunakan dan kinerjanya akan meningkat seiring dengan bertambahnya usia tanaman kayu putih yang digunakan sebagai sumber bahan baku.

Austropuccinia psidii is a pathogenic fungus that causes rust in the Myrtaceae plant. The extensive plantation of the host of this fungus has increased the attack of fungal pathogen, therefore, will increase the threat tothe presence of Myrtaceae species around the globe including in Indonesia. This present study was aiming to detect and identify the presence of this pathogen by morphologicaland molecular observation. Morphological observationrevealed the presence of A. psidii urediniospores onsalam (Syzygium polyanthum) andkayuputih (Melaleuca cajuputi)leaves collected from the arboretum of the Indonesian Center for Forest Biotechnology and Tree Improvement (CFBTI), and the presence of teliospores on young Syzygium leaves. PCR amplification using specific primers of Ppsi1 / Ppsi6 succeeded in detecting the presence of A. psidii fungi Melaleuca and Syzygium showed by DNA amplicon length around 500bp. Efforts to obtain ITS DNA sequences to compare the molecular characteristics of fungi from two different hosts have been carried out, however, the sequencing electropherogram was unreadable, so the comparison can not be performed. This study reported that A. psidii is currently present in Myrtaceae species in Yogyakarta, therefore precaution efforts should be conducted to avoid economic and ecological impact from this pathogen.

Laboratory experiments were carried out to find out the toxicity and biochemical effects of the essential oils (EO) of Anethum graveolens and Melaleuca cajuputi on lesser grain borer Rhyzopertha dominica. Fumigation toxicity experiment was carried out against R. dominica adults by using the essential oils of A. graveolens and M. cajuputi showed 100% and 72% mortality respectively in 40 μl/L at 24 h. Biochemical studies such as total protein, acetylcholinesterase, alpha and beta carboxylesterase, glutathione-s-transferase were carried out to evaluate the impact of essential oils on R. dominica adult. The experimental results showed that the protein content in lethal concentration (LC50) of A. graveolens (17.32 μg/μl) and M. cajuputi (15.72 μg/μl) oils decreased about 4.75%, 13.58% respectively, when compared to control (18.19 μg/μl). Both the essential oils were disturbed the normal acetylcholinesterase, carboxylesterase and glutathione-s-transferase activity when compared to control. These results proved that A. graveolens essential oils showed the potential to control experimented insect pest; hence this essential oil may be used as an alternate for synthetic insecticide to control the R. dominica.

Understanding the population structure is one of the best ways to recognize the population in a plant as a whole. The riverside Maluka of Tanah Laut District is the swamps and river found herbs having an important role to play in maintaining the structure of the soil from the erosion of the river running on the area. Employing the descriptive method, this research attempts to describe population of structure plants of the riverside Maluka. The sample of structure plants are those on both edges of the riverside, namely: 1500 meters long and 2 meters wide. The data were collected by using the transect The results showed that there were 13 plants species trees with the different population structure. There are four plants with population structure of polygonnamely; Mangifera indica, Flacourtia rukam, Antidesma Ghaesembilla, and Melaleuca cajuputi. There are six plants of width-based population structure of width, namely; Gluta renghas, Acacia auriculiformis, Syzygium sp., Syzygium guineense, Pisonia sp., Cassia alata. There are two plants in the forms of basin or pitcher, namely; Mangifera casturi and Fagraea crenulata. Plants that have population structure of reversed polygon is called Lagerstroemia speciosa.

Cajuput oil is a commodity of non-timber forest product which is needed and potential to be developed in Indonesia. Therefore, further research on the factors of production and post-production are needed to produce optimum quality. In this study, the leaves of cajuput (Melaleuca cajuputi Powell) were distillated by water-steam distillation. This study evaluated effects of distillation tank density (60%, 70%, and 80%) and oil storage time (0 month, 1 month, 2 months, and 3 months) on physicochemical properties and chemical compositions of cajuput oils. The results showed that cajuput oils had a specific gravity of 0.915~0.923; optical rotation of (-2,10°) ~ (-1,20°); refractive index of 1.463~1.464; solubility in alcohol 1 : 1; cineole contents of 61.18~76.24%; clear to yellowish clear color; distinctive smell of cajuput and in accordance with SNI 06-3954- 2006. The GC-MS analysis identified 24 of chemical components on the cajuput oils with main components were 1,8-cineole, α-pinene, and β-caryophylene. Tank density 70% to 80% with the oil storage time up to 3 months still gives the optimum quality and chemical compositions.

Gelam tree or Melaleuca cajuputi (M. cajuputi) is an important species for the local economy as well as coastal ecosystem protection in Terengganu, Malaysia. This study aimed at producing a current habitat suitability map and predicting future potential habitat distribution for M. cajuputi in Terengganu based on Species distribution modeling (SDM) using the Maximum Entropy principle. Our modeling results show that for the current potential distribution of M. cajuputi species, only 10.82% (1346.5 km2) of Terengganu area is suitable habitat, which 0.96% of the areas are under high, 2.44% moderate and 7.42% poor habitat suitability. The model prediction for future projection shows that the habitat suitability for M. cajuputi would decrease significantly in the year 2050 under RCP 4.5 where the largest contraction from suitable to unsuitable habitat area is about 442.1 km2 and under RCP 2.6 is the highest expansion from unsuitable to suitable habitat area (267.5 km2). From the future habitat suitability projection, we found that the habitat suitability in Marang would degrade significantly under all climate scenarios, while in Setiu the habitat suitability for M. cajuputi remains stable throughout the climate change scenarios. The modeling prediction shows a significant influence on the soil properties, temperature, and precipitation during monsoon months. These results could benefit conservationist and policymakers for decision making. The present model could also give a perception into potential habitat suitability of M. cajuputi in the future and to improve our understanding of the species’ response under the changing climate.

Plants in the genus Melaleuca have been widely used as traditional medicine mainly because of their broad spectrum antimicrobial activity. In this study, we reported the complete chloroplast genome of Melaleuca cajuputi subsp. cumingiana. The chloroplast genome of this species is 158,855 bp in length, including a pair of inverted repeat regions (IRs) (26,727 bp) that is divided by a large single-copy (LSC) area (87,338 bp) and a small single-copy (SSC) area (18,063 bp). The circular chloroplast genome of M. cajuputi subsp. cumingiana contains 135 unique genes, composing of 87 protein-coding genes, 40 tRNA genes, and eight rRNA genes. Phylogenetic analysis indicates that M. cajuputi subsp. cumingiana was clustered with species in the tribe Melaleuceae. This complete chloroplast genome of M. cajuputi subsp. cumingiana will provide a powerful tool to accelerate breeding, biotechnological and phylogenetic study.

Melaleuca cajuputi essential oil (MCEO) from Pulau Buru (PBR), Indonesia, has been used as a functional flavor on Cajuputs Candy for years. Our recent study has explored thirteen other alternative MCEO sources to be developed as food flavor. However, not all of the MCEO had similarities to PBR MCEO both in their sensory and volatile profiles. This study aimed to identify the aroma-active compounds which would affect the overall aroma perception of the most- and the least-liked MCEO as a flavor ingredient based on the nasal impact frequency (NIF) method. Initial screening was performed to evaluate the overall liking of thirteen MCEO on Cajuputs Candy through a hedonic test, conducted by seventy-eight untrained panelists. The sample obtained from Mojokerto (MOJ) was the most-liked alternatives MCEO in a similar degree of liking to the currently used PBR, while Belu (BEL) was the least-liked. Further analysis using Gas chromatography–Mass spectrometry/Olfactometry (GC-MS/O) was carried out on the MCEO from PBR, MOJ, as well as BEL. The response of nine panelists showed that the overall aroma perception of PBR was contributed by 1,8 cineole (eucalyptus-like, mint, fresh), α-pinene (pine, green, fresh), and ylangene (spicy, fresh, woody) as the main aroma-active compounds. In addition to α-pinene and 1,8 cineole, the unique aroma of MOJ was dominantly contributed by caryophyllene, possessing a woody, sweet, and spicy aroma. The strong floral odor of linalool and nerolidol, the aroma-active compounds of BEL, generated distinct sensory characteristics in comparison to the reference, PBR.

Land use changes from Malaleuca cajuputi swamp forest ecosystem to agriculture production area and settlement land decrease habitat of proboscis monkeys. The habitat position at the edge of the river and high accessibility cause d faster habitat damage. In addition, forest fires occurred in 2015 has caused significant losses of flora and fauna biodiversity in the area. In order to know the type of plants in the study site, the vegetation analysis was conducted. The study site consisted of 19 species of lower plant and 4 types of trees. Dominant species and codominant of study site are widely distributed, indicating the existence of competition in obtaining nutrients and space. At the under storey plant level was dominated by Stenochlaena palustris and Scirpus grossus at the locations A and B whereas at location C was dominated by the Eleocharis dulcis and Jussieua erecta . In the types of trees, the dominant trees were Melaleuca cajuputi , Muntingia sp , Alstonia angustifolia , Shorea balangeran , and Albizia falcataria . In all three locations, there were low similarity values in the plant species below, the level of seedlings, and samplings.

Essential oil extracted from Melaleucagenus has been widely used worldwidefor many purposes. Most of the its has been reported as the source of phytochemical compound where mostly have anti-inflammatory, antiulcer, antioxidant, antimicrobial and insecticide properties. Thus, three objectives that have been highlighted in this study are (1) to extract essential oil (cajuput oil) from fresh leaves of MelaleucacajuputiPowell using simple steam distillation,(2) to determine the chemical components of MelaleucacajuputPowell essential oil using GCMS and (3) to compare the chemical compound and its percentage with the previous study.Results found that there were 41 chemical compounds that have been identified. The chemical compounds were monoterpenes such as α-terpinolene, α-pinene, sabinene, 4-terpineol and γ-terpinene. The highest chemical compounds found were caryophyllene (20.16%), α-terpinolene (17.0%),α-humulene (11.91%), β-elemene (7.62%) and γ-terpinene (5.62%). In previous research, 1,8-cineole chemical compound was foundhowever, in this study a different chemical compound found, it was caryophyllene, this is due to different geographic area.As a conclusion, most of the compounds found was aromatic, antibacterial andinsecticide properties.

The aim of this study was to evaluate the in vitro activity of Melaleuca cajuputi essential oil leaf extract against Aedes aegypti and Aedes albopictus. The essential oil of the M. cajuputi was obtained via steam hydro-distillation and analyzed by GC-MS for the chemical constituents. GC-MS analysis showed that the essential oil of M. cajuputi contained 13 compounds with two major chemical constituents, 2-propenoic acid (29.55%) and caryophyllene (20.04%). Adulticidal and larvicidal bioassay was evaluated following WHO guidelines. Larvicidal bioassays were conducted with the 10, 50, 80, 100, and 120 mg/L M. cajuputi essential oil against late 3rd instar of Ae. aegypti and Ae. albopictus which give LC50 120.99 mg/L and 222.58 mg/L, respectively. Adult mortality was observed after 24 h with the LC50 of 0.029 mg/cm2 for Ae. aegypti and 0.028 mg/cm2 for Ae. albopictus. In adulticidal bioassay, M. cajuputi essential oils showed effective results at 0.04 mg/cm2 concentrations, with values for KT50 of 77.71 min for Ae. aegypti and 69.49 min for Ae. albopictus. M. cajuputi essential oils exhibited moderate toxicity effects against the larva and adults of Aedes species and may be used as an alternative to chemical insecticide.

This study evaluates the effects of different extracts from leaves (fresh and dry) and roots of melaleuca on pathogens such as Meloidogyne spp., Phytophthora spp. and Fusarium spp. which affect pepper orchards in Hau Giang province, Vietnam. The results showed that the extracts increased the rate of death of a lethal nematode (Meloidogyne spp.) on pepper up to 100%, while the natural death rate (control) only reached to58% at 3 days after stocking in vitro, and the extracts from fresh leaves had the quickest response. However, extracts from both the melaleuca fresh leaves and roots were not effective on the development of all 11 strainsof Fusarium spp. and 5 strains of Phytophthora spp. which were tested after 3 days of inoculation. The results illustrated that the extracts of dried leaves was positive against the growth of 2 strains (18%), increased thegrowth of 8 strains (73%) and ineffective on 1 strain (9%) of Fusarium spp., and completely ineffective on all 5 strains of Phytophthora spp.

Melaleuca cajuputi has an ability to sprout easily, this is one of indicator for plants that potentially could be reproduced productively. PT Bukit Asam Ltd. has used Melaleuca cajuputi as the main of tree species for the reclamation of its post-mining land. The seedlings are produced generatively using seeds. The purpose of this research was to determine responses of IBA and Growth Media on the tip cutting of Melaleuca cajuputi The results showed that 88.9% of the cuttings, 61.2% of the rooted, and 80.9% of the shoots were viable. The used of IBA and a variety of growing media did not significantly affect to life percentage, rooted percentage, shoots percentage, fresh weight and dry weight of root, fresh weight and dry weight of shoots, numbers of primary and secondary roots, length of primary roots, and length of shoots. The result of this research can be adopted by PT Bukit Asam Ltd. to shorten production time of Melaleuca cajuputi seedlings in the future, where the availability of seeds is limited.Keywords: IBA, Melaleuca cajuputi, cutting, mining

Background: Cajuput oil is a traditional and famous product of Thua Thien Hue, it is considered as a potential product, contributing to the economic development of Thua Thien Hue. Currently, there are many different products of cajuput oil in the market. However, the quality of cajuput oils in the production facilities has not been considered. The aim of the present study was to apply GC to the determination of the main components of commercial cajuput oils from M. cajuputi species in the Thua Thien Hue province. Materials and method: The chemical composition of 10 commercial cajuput oils extracted from leaves of Melaleuca cajuputi Powell collected in the Thua Thien Hue province was determined by gas chromatography. Results and conclusion: The major componentsofthe oils wereα-pinene, β-pinene, limonene, 1,8-cineoleand terpineol. The result obtained demonstrate that 1,8-cineole is the main component,present in 10 samples with values ranging from 15 to 59%. Key words: Cajuput oil, Melaleuca cajuputi, 1,8-cineole, α-pinene, β-pinene, limonene, terpineol

The increasing demand for global energy and declining oil reserves have driven the search for alternative biofuel as fossil fuel replacement. This study proposes new blends of a light biofuel based on Melaleuca Cajuputi oil (MCO) and refined palm oil (RPO) that has lowered viscosities and densities properties as compared to neat RPO. In addition, a statistical approach using mixture design method was used in the design of experiment (DOE) to evaluate the correlation between the ratio of RPO-MCO blends on their kinematic viscosity and density properties. Based on the mixture design algorithm, a total of eight experimental runs were conducted consisting of three combinations of RPO-MCO blends, two neat oils and three replicate samples. A good fit between the developed model and the experimental data was obtained for the models of kinematic viscosity and density, with corresponding R2 values 0.9992 and 0.9998, respectively. Discrepancies in the predicted values to the experimental results were within 0.1–2.5%, validating the accuracy of the developed models. The optimal blend calculated is 32RPO68MCO which gives 5.9 mm2/s and 881.5 kg/m3 for the response values of kinematic viscosity and density, respectively. Both responses values satisfy the requirement of the ASTM D6751/EN14214 standard for biodiesel.

Melaleuca forests significantly contribute to economic development through silviculture and play an important role in carbon sequestration and supply of oxygen. Currently, Melaleuca seedlings are planted on bunds in inundated areas or on trenches in elevated areas. Little is known of what planting methods and growth stage of Melaleuca trees are best for developing new forest areas and sequestering highest levels of carbon. We selected U Minh Ha National Park in search for answers to the questions. We established 12 standard plots (10m x 10m) in the park where Melaleuca trees at two ages (10 years of age and 15 years of age) were planted on bunds and trenches. We employed Anova analysis, SPSS software and numerical analysis for analyzing and evaluating carbon sequestration by Melaleuca trees. We found that planting method significantly contributes to developing new forest areas in terms of tree volume, biomass, and carbon sequestration. Bund-based planting proves to be more cost-effective in developing new forest areas and carbon sequestration than trench-based planting. Trees at the age of 10 planted on bunds has been the most effective category for sequestering carbon. Our methodology in examining the relationship proves to be a feasible reference to new forest development and payment for forest service scheme in the Mekong Delta region or elsewhere in the broader region.

Waste of cajuput leaves (Melaleuca cajuputi Powell) has the potential to be used as an alternative cattle feed because it has a fairly high nutritional content. The nutritional content of cajuput leaf waste needs to be improved before being used as an alternative feed. The fermentation process can increase nutrient content in cajuput leaf waste and increase the digestibility of organic matter (OMD), dry matter digestibility (DMD), Ammonia (NH3), and Volatile Fatty Acid (VFA). This study aimed to determine the improvement of cajuput leaf waste nutrition with the help of yeast as well as the effect of fermented cajuput leaf waste along with a mixture of concentrates and field grass on digestibility, NH3, and VFA which were carried out in vitro test. The results showed the nutritional content of cajuput leaf waste fermented by yeast of Aspergillus chevalieri had the best value of protein 16.03%, fiber 16.92%, and fat 5.93%. The treatment R4 (50% cajuput leaf waste + 25% concentrate + 25% field grass) had the best digestibility (DMD=46.12%; DMO=32.08%), NH3 (8.37mM) and VFA (168.5mM) production.

Background: The main problem in nitrogen fertilization for crop cultivated is the very low efficiency due to the leaching process. The purpose of this study was to the determination of the optimum levels of biochar amendment made from Melaleuca cajuputi biochar (MCB) and urea fertilizer (UF) for nitrogen use efficiency in upland rice under M. cajuputi stands.Methods: The study was conducted during dry season within March to June 2019 in Menggoran Forest Resort, Playen Forest Section, Yogyakarta Forest Management District, Indonesia. The experimental design was laid out in a randomized complete block design factorial with three replications as the response surface methodology (RSM). The treatments consisted of MCB levels (0, 5, 10, 15 tons ha-1) and UF levels (0, 100, 200, 300 kg ha-1) as independent variables. The observation parameters were nitrate reductase activity (NRA), total chlorophyll (TC), leaf photosynthesis rate (LPR), nitrogen loss (NL), nitrogen use efficiency (NUE) and seed yield (SY). The data was analyzed using RSM approach and ridge regression.Result: The optimizing applications of 11.14 tons ha-1 of MCB and 281.13 kg ha-1 of UF resulted in NUE and SY by 2.14 kg grain kg Nfertilizer-1 and 5.83 tons ha-1 or increased by 19.07% and 13.02%, compared to a single application of UF by 300 kg ha-1.

Naresuan University Journal: Science and Technology, 29, 2, 64-72

Three new β-triketone flavanone hybrids, cajuputones A-C were obtained from Melaleuca cajuputi (the Australian 'tea tree'). The structures of cajuputones A-C were elucidated by 1D/2D NMR spectroscopy and HR-ESI-MS analyses; and their absolute configurations were established by electric circular dichroism (ECD) calculations using TDDFT method. Structurally, cajuputones A-C feature a rare 6/6/6/6 oxatetracyclic ring system fused between an acylphloroglucinol-derived β-triketone and a pinocembrin or strobopinin moiety via an angle-type pyran-like motif. DFT-based conformational optimization in chloroform explained the similarity of the 1D NMR data of cajuputones B and C (C-2 epimers).

Suryanto P, Taryono, Supriyanta, Kastono D, Putra ETS, Widyawan MH, Alam T. 2020. Assessment of soil quality parameters and yield of rice cultivars in Melaleuca cajuputi agroforestry system. Biodiversitas 21: 3463-3470. Interactions between rice cultivars and soil quality parameters rises problems in the attempt of increasing rice yield. The objective of this study was to assess soil quality parameters that affect the yield of 15 rice cultivars in an agroforestry system of ‘kayu putih’ (Melaleuca cajuputi) situated in Menggoran forest, Yogyakarta, Indonesia which have three soil types namely Lithic Haplusterts, Ustic Epiaquerts, and Vertic Haplustalfs. The observation was conducted on 21 soil quality parameters and yield of rice cultivars. The data were analyzed by using ANOVA, factor analysis, and stepwise regression. The highest yield of rice per hectare was attained by GM 28 in Ustic Epiaquerts with 6.493 tons ha-1, while Situ Patenggang and GM 28 in Vertic Haplustalfs as high as5.549 and 5.401 tons ha-1, respectively, and Situ Patenggang in Lithic Haplusterts as high as 4.893 tons ha-1. Soil quality parameters that had significant effect on the yield of rice cultivars were Clay, SMC, pH, SOC, N, Mg, Fe, Fg, and Bae. We suggested that rice cultivars recommendations for Lithic Haplusterts, Ustic Epiaquerts, and Vertic Haplustalfs are Situ Patenggang, Situ Patenggang or GM 28, and GM 28, respectively, in addition to fertilization based on limiting factors of each rice cultivars.

Due to food security issues and weeds problem in rice production, allelopathy is introduced as an alternative towards sustainable weeds management. One of Myrtacea plants known as Melaleuca cajuputi extract is studied to assess its herbicidal or allelopathic effect on notorious paddy weeds known as barnyard grass. The plant extract of Melaleuca cajuputi is extracted using supercritical carbon dioxide extraction and soxhlet extraction. The chemical composition of the extract is determined using gas chromatograph mass spectrometry analysis and it was reported that caryophyllene and humulene are detected as the two major sesquiterpenes present. The effectiveness of the inhibition of the weeds was also studied and compared with conventional herbicides and caryophyllene standards. It is determined that Melaleuca cajuputi extract of supercritical and soxhlet extraction induces visible injuries towards the weeds at bud stage of less than 0.75 cm. Thus, it is recommended to increase the concentration higher than 0.05 M to suppress the weeds that grown. Melaleuca cajuputi oil can be further studied to be a potential source for bio-herbicides.

Suryanto P, Faridah E, Nurjanto HH, Supriyanta, Kastono D, Putra ETS, Handayani S, Dewi AK, Alam T. 2020. Influence of siam weed compost on soybean varieties in an agroforestry system with kayu putih (Melaleuca cajuputi). Biodiversitas 21: 3062-3069. Siam weed (Chromolaena odorata (L.) R.M.King & H.Rob.) has grown wild in many kayu putih (Melaleuca cajuputi Powell) forest can be utilized as compost for complementary of inorganic fertilizers in annual crops. The experiment was conducted during November-February 2020 in Menggoran Forest Resort, Playen Forest Section, Yogyakarta Forest Management District, Indonesia. The experiment was arranged in a randomized complete block design (RCBD) with three replications. The first factor was soybean varieties consisted of Anjasmoro, Dering I, and Grobogan. The second factor was siam weed compost (SWC) application consisted of 0, 5, 10, and 15 tons ha-1. The data were analyzed using Two-way ANOVA, ANCOVA, and stepwise regression. The SWC application of 10 tons ha-1 showed the highest yield of Anjasmoro, Dering I, and Grobogan were 1.42, 1.56, and 1.51 tons ha-1, respectively, or increased by118.46%, 102.60%, and 112.68%, respectively, compared to the without SWC application. The optimum dosage of SWC application for Anjasmoro, Dering I, and Grobogan were 13.05, 14.35, and 14.93 tons ha-1, respectively, with a maximum yield of 1.45, 1.59, and 1.52 tons ha-1, respectively. Soil quality and physiological parameters that had a significant influenced on the production of soybean varieties in agroforestry systems with M. cajuputi were SOM, K, LPR, TC, and PRO.

GC-MS was applied to identify 24 main substances in Melaleuca cajuputi essential oil (TA) extracted from fresh cajeput leaves through steam distilling. The inhibitory capability of active compounds in the TA from Thua Thien Hue, Vietnam over the Angiotensin-Converting Enzyme 2 (ACE2) protein in human body - the host receptor for SARS-CoV-2 and the main protease (PDB6LU7) of the SARS-CoV-2 using docking simulation has been studied herein. The results indicate that the ACE2 and PDB6LU7 proteins were strongly inhibited by 10 out of 24 compounds accounting for 70.9% in the TA. The most powerful anticoronavirus activity is expressed in the order: Terpineol (TA2) ≈ Guaiol (TA5) ≈ Linalool (TA19) > Cineol (TA1) > β-Selinenol (TA3) > α-Eudesmol (TA4) > γ-Eudesmol (TA7). Interestingly, the synergistic interactions of these 10 substances of the TA exhibit excellent inhibition into the ACE2 and PDB6LU7 proteins. The docking results orient that the natural Melaleuca cajuputi essential oil is considered as a valuable resource for preventing SARS-CoV-2 invasion into human body.

Widiana A, Ukit, Thahirah A, Paijiah E. 2019. Diversity of endophytic fungi in cajuput leaf waste (Melaleuca cajuputi). Biodiversitas 20: 562-569. Cajuput Oil Factory at Jatimunggul, Indramayu of Forest Management Unit of Perum Perhutani (State Forest Enterprises) produces cajuput leaf waste during distillation process. This waste is difficult to decompose due to the presence of lignin in the secondary cell wall. Therefore, fungi are needed to produce some enzymes for speeding up the degrading process. This research was aimed at finding out the diversity of fungi growing in the cajuput (Melaleuca cajuputi Powell) leaf waste of zero, two and four months old. The result indicated that a total of 14 species of fungi belonging to four genera namely Aspergillus, Arthrinium, Rhizopus, and one unidentified genus are produced by cajuput leaf waste. Ten different types of endophytic fungi were isolated from two-month-old waste, seven Rhizopus and types of fungi were found in the zero-month-old waste, and only five types of isolates are found in the four-month-old waste. Three species of isolated fungi were also tested for their cellulase enzyme activity.