1. MiR-186 inhibits cell proliferation and invasion in human cutaneous malignant melanoma.
- Author
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Su BB, Zhou SW, Gan CB, and Zhang XN
- Subjects
- Apoptosis genetics, Cell Cycle Checkpoints genetics, Cell Line, Tumor, Cell Movement, Cell Proliferation, Gene Expression, Gene Expression Regulation, Neoplastic, Humans, Melanoma pathology, Skin Neoplasms pathology, Transfection, Melanoma, Cutaneous Malignant, Melanoma genetics, MicroRNAs genetics, RNA Interference, Skin Neoplasms genetics
- Abstract
Aims: MicroRNA-186 (miR-186) has been shown to be involved in various types of cancer. The purpose of this study was to investigate the expression level and functional role of miR-186 in human cutaneous malignant melanoma cells., Subjects and Methods: Expression of miR-186 was analyzed in human cutaneous malignant melanoma (CMM) cell lines SK-MEL-1, G-361, A375 and A875, and human normal epidermal melanocytes cell line HEMn-LP by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Additionally, the functional role of miR-186 on melanoma cells was investigated by transfection of miR-186 mimic followed by analyses of cell proliferation, apoptosis, and metastasis., Results: We found that the expression levels of miR-186 were decreased in CMM cell lines compared with normal epidermal melanocytes cell line. Moreover, overexpression of miR-186 inhibited cells proliferation through abrogating the G
1 -S transition, and reduced cells migration and invasion., Conclusions: Our findings suggested that miR-186 exhibit an inhibitory effect on CMM cell proliferation, migration, and invasion; thus, may serve as a potential therapeutic target for human CMM intervention., Competing Interests: There are no conflicts of interest- Published
- 2018
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