1. Responses of the grass Paspalum distichum L. to Hg stress: A proteomic study.
- Author
-
Ding W, Zhang J, Wu SC, Zhang S, Christie P, and Liang P
- Subjects
- Biodegradation, Environmental, Down-Regulation, Gene Expression Regulation, Plant drug effects, Mercury metabolism, Oxidative Stress genetics, Paspalum genetics, Paspalum metabolism, Photosynthesis drug effects, Photosynthesis genetics, Plant Leaves metabolism, Plant Proteins genetics, Plant Roots drug effects, Plant Roots metabolism, Proteomics, Soil Pollutants metabolism, Up-Regulation, Mercury toxicity, Oxidative Stress drug effects, Paspalum drug effects, Plant Proteins metabolism, Soil Pollutants toxicity
- Abstract
Paspalum distichum L. was tested to evaluate its ability to phytoremediate mercury (Hg) contaminated soil over a 60-d period by analysis of the total Hg concentrations in roots and leaves. Hg concentration in Hg-contamination soil decreased by 70.0 μg g
-1 after 60 day of grass cultivation and Hg was readily taken up by the roots (4.51 ± 1.90 μg g-1 ) rather than the leaves (0.35 ± 0.02 μg g-1 ). In addition, a comparative proteomic study was performed to unravel the protein expression involved in the Hg stress response in P. distichum L. A total of 49 proteins were classified as differentially proteins in the roots by the 'top three' proteomic analysis, of which 32 were up-regulated and 17 down-regulated in response to Hg stress. These changed proteins were classified by gene ontology analysis into five complex molecular functions involving photosynthesis and energy metabolism (31%), oxidative stress (14%), protein folding (16%), sulfur compound metabolism (10%), metal binding, and ion transport (29%). Moreover, the protein expression patterns were consistent with the metabolism pathway results. Overall, the results contribute to our understanding of the molecular mechanisms of the Hg response in P. distichum and we propose a theoretical basis for the phytoremediation of Hg-contaminated soils., (Copyright © 2019 Elsevier Inc. All rights reserved.)- Published
- 2019
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