1. Polydopamine-Laced Biomimetic Material Stimulation of Bone Marrow Derived Mesenchymal Stem Cells to Promote Osteogenic Effects.
- Author
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Lee DJ, Lee YT, Zou R, Daniel R, and Ko CC
- Subjects
- Animals, Bone Marrow Cells drug effects, Calcification, Physiologic drug effects, Cell Adhesion drug effects, Cell Proliferation drug effects, Cell Survival drug effects, Collagen pharmacology, Compressive Strength, Durapatite pharmacology, Fluorescence, Male, Mesenchymal Stem Cells drug effects, Mesenchymal Stem Cells metabolism, Rats, Sprague-Dawley, Skull drug effects, Skull pathology, Surface Properties, X-Ray Microtomography, Biomimetic Materials pharmacology, Bone Marrow Cells cytology, Indoles pharmacology, Mesenchymal Stem Cells cytology, Osteogenesis drug effects, Polymers pharmacology
- Abstract
A hydroxyapatite-collagen (HC) composite material can mimic composition and ultra-structures of natural bone and provide adequate bioactive material-tissue interactions. Incorporation of dopamine (DA) is one of keys in increasing the mechanical strength of the HC material to approaching that of cortical bone. In this study, the in vitro osteogenic effects of polydopamine-laced hydroxyapatite collagen calcium silicate (HCCS-PDA) were examined by culturing rat mesenchymal stem cells (rMSCs) on HCCS-PDA and HCCS coated plates. HCCS-PDA group demonstrated less cytotoxic from Live/Dead cytotoxic assay and displayed higher cell attachment, proliferation and mineralization than the HCCS group in vitro. For in vivo bone regeneration, HCCS-PDA or HCCS particulates with or without rMSC aggregates were implanted into rat critical-sized calvarial defects (CSD). After 12 weeks, calvarial bone regeneration was evaluated radiographically, histologically, and histomorphometrically. While the majority of new bone formation occurred around the HCCS-PDA particulates with rMSC aggregates, The HCCS-PDA particulates without rMSC aggregates showed limited osteoconductivity. HCCS with or without rMSC aggregates resulted in less bone formation, indicating a prominent role of DA in effective bone regeneration. Therefore, the HCCS-PDA biomaterial with the aid of rMSCs can be used to develop therapeutic strategies in bone tissue engineering with numerable clinical applications.
- Published
- 2017
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