12 results on '"Wong, Justin J-L"'
Search Results
2. A multiomics dataset for the study of RNA modifications in human macrophage differentiation and polarisation.
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Pinello, Natalia, Song, Renhua, Lee, Quintin, Calonne, Emilie, Larance, Mark, Fuks, François, and Wong, Justin J. -L.
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RNA modification & restriction ,MULTIOMICS ,RNA metabolism ,MACROPHAGES ,MESSENGER RNA ,MONOCYTES - Abstract
RNA modifications have emerged as central regulators of gene expression programs. Amongst RNA modifications are N6-methyladenosine (m
6 A) and RNA 5-hydroxymethylcytosine (5hmC). While m6 A is established as a versatile regulator of RNA metabolism, the functions of RNA 5hmC are unclear. Despite some evidence linking RNA modifications to immunity, their implications in gene expression control in macrophage development and functions remain unclear. Here we present a multi-omics dataset capturing different layers of the gene expression programs driving macrophage differentiation and polarisation. We obtained mRNA-Seq, m6 A-IP-Seq, 5hmC-IP-Seq, Polyribo-Seq and LC-MS/MS data from monocytes and resting-, pro- and anti-inflammatory-like macrophages. We present technical validation showing high quality and correlation between samples for all datasets, and evidence of biological consistency of modelled macrophages at the transcriptomic, epitranscriptomic, translational and proteomic levels. This multi-omics dataset provides a resource for the study of RNA m6 A and 5hmC in the context of macrophage biology and spans the gene expression process from transcripts to proteins. [ABSTRACT FROM AUTHOR]- Published
- 2024
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3. Overexpression of VIRMA confers vulnerability to breast cancers via the m6A-dependent regulation of unfolded protein response.
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Lee, Quintin, Song, Renhua, Phan, Dang Anh Vu, Pinello, Natalia, Tieng, Jessica, Su, Anni, Halstead, James M., Wong, Alex C. H., van Geldermalsen, Michelle, Lee, Bob S.-L., Rong, Bowen, Cook, Kristina M., Larance, Mark, Liu, Renjing, Lan, Fei, Tiffen, Jessamy C., and Wong, Justin J.-L.
- Abstract
Virilizer-like m
6 A methyltransferase-associated protein (VIRMA) maintains the stability of the m6 A writer complex. Although VIRMA is critical for RNA m6 A deposition, the impact of aberrant VIRMA expression in human diseases remains unclear. We show that VIRMA is amplified and overexpressed in 15–20% of breast cancers. Of the two known VIRMA isoforms, the nuclear-enriched full-length but not the cytoplasmic-localised N-terminal VIRMA promotes m6 A-dependent breast tumourigenesis in vitro and in vivo. Mechanistically, we reveal that VIRMA overexpression upregulates the m6 A-modified long non-coding RNA, NEAT1, which contributes to breast cancer cell growth. We also show that VIRMA overexpression enriches m6 A on transcripts that regulate the unfolded protein response (UPR) pathway but does not promote their translation to activate the UPR under optimal growth conditions. Under stressful conditions that are often present in tumour microenvironments, VIRMA-overexpressing cells display enhanced UPR and increased susceptibility to death. Our study identifies oncogenic VIRMA overexpression as a vulnerability that may be exploited for cancer therapy. [ABSTRACT FROM AUTHOR]- Published
- 2023
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4. Interplay between N6-adenosine RNA methylation and mRNA splicing.
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Mehravar, Majid and Wong, Justin J-L
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RNA modification & restriction , *RNA methylation , *CELL physiology , *MESSENGER RNA , *PROTEINS , *RNA splicing - Abstract
N 6 -methyladenosine (m6A) is the most abundant modification to mRNAs. Loss-of-function studies of main m6A regulators have indicated the role of m6A in pre-mRNA splicing. Recent studies have reported the role of splicing in preventing m6A deposition. Understanding the interplay between m6A and mRNA splicing holds the potential to clarify the significance of these fundamental molecular mechanisms in cell development and function, thereby shedding light on their involvement in the pathogenesis of myriad diseases. • m6A RNA modification and mRNA splicing have been reported to regulate each other. • Splice site m6A has been shown to regulate splicing via specific m6A reader proteins. • Splicing inhibits m6A deposition in short exons. • Profiling m6A on nascent mRNAs may reveal a broader role of m6A in splicing regulation. [ABSTRACT FROM AUTHOR]
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- 2024
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5. The Activity-Induced Long Non-Coding RNA Meg3 Modulates AMPA Receptor Surface Expression in Primary Cortical Neurons.
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Tan, Men C., Widagdo, Jocelyn, Chau, Yu Q., Tianyi Zhu, Wong, Justin J.-L., Cheung, Allen, and Anggono, Victor
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NON-coding RNA ,AMPA receptors ,NEUROPLASTICITY ,MESSENGER RNA ,METHYL aspartate receptors - Abstract
Transcription of new RNA is crucial for maintaining synaptic plasticity, learning and memory. Although the importance of synaptic plasticity-related messenger RNAs (mRNAs) is well established, the role of a large group of long non-coding RNAs (lncRNAs) in long-term potentiation (LTP) is not known. In this study, we demonstrated the expression of a lncRNA cluster, namely maternally expressed gene 3 (Meg3), retrotransposon-like gene 1-anti-sense (Rtl1-AS), Meg8 and Meg9, which is located in the maternally imprinted Dlk1-Dio3 region on mouse chromosome 12qF1, in primary cortical neurons following glycine stimulation in an N-Methyl-D-aspartate receptor (NMDAR)-dependent manner. Importantly, we also validated the expression of Meg3, Meg8 and Meg9 in the hippocampus of mice following cued fear conditioning in vivo. Interestingly, Meg3 is the only lncRNA that is expressed in the nucleus and cytoplasm. Further analysis revealed that Meg3 loss of function blocked the glycine-induced increase of the GluA1 subunit of AMPA receptors on the plasma membrane, a major hallmark of LTP. This aberrant trafficking of AMPA receptors correlated with the dysregulation of the phosphatidylinoside-3-kinase (PI3K)/AKT signaling pathway and the downregulation of the lipid phosphatase and tensin homolog (PTEN). These findings provide the first evidence for a functional role of the lncRNA Meg3 in the intricate regulation of the PTEN/PI3K/AKT signaling cascade during synaptic plasticity in neurons. [ABSTRACT FROM AUTHOR]
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- 2017
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6. Genetic alterations of m6A regulators predict poorer survival in acute myeloid leukemia.
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Chau-To Kwok, Marshall, Amy D., Rasko, John E. J., and Wong, Justin J. L.
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ACUTE myeloid leukemia ,METHYLATION ,ADENOSINES ,MESSENGER RNA ,CANCER - Abstract
Methylation of N
6 adenosine (m6 A) is known to be important for diverse biological processes including gene expression control, translation of protein, and messenger RNA (mRNA) splicing. However, its role in the development of human cancers is poorly understood. By analyzing datasets from the Cancer Genome Atlas Research Network (TCGA) acute myeloid leukemia (AML) study, we discover that mutations and/or copy number variations of m6 A regulatory genes are strongly associated with the presence of TP53 mutations in AML patients. Further, our analyses reveal that alterations in m6 A regulatory genes confer a worse survival in AML. Our work indicates that genetic alterations of m6 A regulatory genes may cooperate with TP53 and/or its regulator/downstream targets in the pathogenesis and/or maintenance of AML. [ABSTRACT FROM AUTHOR]- Published
- 2017
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7. Intron retention in mRNA: No longer nonsense.
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Wong, Justin J.-L., Au, Amy Y. M., Ritchie, William, and Rasko, John E. J.
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INTRONS , *SPLIT genes , *EXONS (Genetics) , *MESSENGER RNA , *RNA splicing - Abstract
Until recently, retention of introns in mature mRNAs has been regarded as a consequence of mis-splicing. Intronretaining transcripts are thought to be non-functional because they are readily degraded by nonsense-mediated decay. However, recent advances in next-generation sequencing technologies have enabled the detection of numerous transcripts that retain introns. As we review herein, intron-retaining mRNAs play an essential conserved role in normal physiology and an emergent role in diverse diseases. Intron retention should no longer be overlooked as a key mechanism that independently reduces gene expression in normal biology. Exploring its contribution to the development and/or maintenance of diseases is of increasing importance. [ABSTRACT FROM AUTHOR]
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- 2016
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8. Identification of nuclear-enriched miRNAs during mouse granulopoiesis.
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Wong, Justin J. L., Ritchie, William, Gao, Dadi, Lau, Katherine A., Gonzalez, Maria, Choudhary, Anupma, Taft, Ryan J., Rasko, John E. J., and Holst, Jeff
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MESSENGER RNA , *CELL proliferation , *BIOLOGY education , *PHYSIOLOGICAL control systems , *CELL growth , *BINDING sites , *CELL culture - Abstract
Background MicroRNAs (miRNAs) are coordinators of cellular differentiation, including granulopoiesis. Although differential expression of many miRNAs is associated with the maturation of granulocytes, analysis of differentially expressed miRNAs and their cellular localization across all stages of granulopoiesis, starting from hemopoietic stems cells, is not well characterized. Methods We analyzed whole cell miRNA and mRNA expression during granulopoiesis using Taqman low-density and Affymetrix arrays respectively. We also performed nuclear and cytoplasmic fractionation followed by Taqman low-density array and/or quantitative PCR to identify nuclear-enriched miRNAs in hemopoietic stem/progenitor cells, promyelocytes, myelocytes, granulocytes and several hemopoietic cell lines. Anti-correlation between the expression of miRNA and target pairs was used to determine putative miRNA targets. Results Analyses of our array data revealed distinct clusters of differentially expressed miRNAs that are specific to promyelocytes and granulocytes. While the roles of many of these miRNAs in granulopoiesis are not currently known, anti-correlation of the expression of miRNA/mRNA target pairs identified a suite of novel target genes. Clusters of miRNAs (including members of the let-7 and miR-17-92 families) are downregulated in hemopoietic stem/progenitor cells, potentially allowing the expression of target genes known to facilitate stem cell proliferation and homeostasis. Additionally, four miRNAs (miR-709, miR-706, miR-690 and miR-467a*) were found to be enriched in the nucleus of myeloid cells and multiple hemopoietic cell lines compared to other miRNAs, which are predominantly cytoplasmic-enriched. Both miR-709 and miR-706 are nuclear-enriched throughout granulopoiesis and have putative binding sites of extensive complementarity downstream of pri-miRNAs. Nuclear enrichment of miR-467a* is specific to hemopoietic stem/progenitors and promyelocytes. These miRNAs are also nuclear-enriched in other hemopoietic cell lines, where nuclear sequestering may fine-tune the expression of cytoplasmic mRNA targets. Conclusions Overall, we have demonstrated differentially expressed miRNAs that have not previously been associated with hemopoietic differentiation and provided further evidence of regulated nuclear-enrichment of miRNAs. Further studies into miRNA function in granulocyte development may shed light on fundamental aspects of regulatory RNA biology and the role of nuclear miRNAs. [ABSTRACT FROM AUTHOR]
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- 2014
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9. Orchestrated Intron Retention Regulates Normal Granulocyte Differentiation.
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Wong, Justin?J.-L., Ritchie, William, Ebner, Olivia?A., Selbach, Matthias, Wong, Jason?W.H., Huang, Yizhou, Gao, Dadi, Pinello, Natalia, Gonzalez, Maria, Baidya, Kinsha, Thoeng, Annora, Khoo, Teh-Liane, Bailey, Charles?G., Holst, Jeff, and Rasko, John?E.J.
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INTRONS , *GRANULOCYTES , *CELL differentiation , *RNA splicing , *MESSENGER RNA , *BIOINFORMATICS , *PROTEOMICS , *GENETIC databases - Abstract
Summary: Intron retention (IR) is widely recognized as a consequence of mis-splicing that leads to failed excision of intronic sequences from pre-messenger RNAs. Our bioinformatic analyses of transcriptomic and proteomic data of normal white blood cell differentiation reveal IR as a physiological mechanism of gene expression control. IR regulates the expression of 86 functionally related genes, including those that determine the nuclear shape that is unique to granulocytes. Retention of introns in specific genes is associated with downregulation of splicing factors and higher GC content. IR, conserved between human and mouse, led to reduced mRNA and protein levels by triggering the nonsense-mediated decay (NMD) pathway. In contrast to the prevalent view that NMD is limited to mRNAs encoding aberrant proteins, our data establish that IR coupled with NMD is a conserved mechanism in normal granulopoiesis. Physiological IR may provide an energetically favorable level of dynamic gene expression control prior to sustained gene translation. [ABSTRACT FROM AUTHOR]
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- 2013
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10. Functional role of Tet-mediated RNA hydroxymethylcytosine in mouse ES cells and during differentiation.
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Lan, Jie, Rajan, Nicholas, Bizet, Martin, Penning, Audrey, Singh, Nitesh K., Guallar, Diana, Calonne, Emilie, Li Greci, Andrea, Bonvin, Elise, Deplus, Rachel, Hsu, Phillip J., Nachtergaele, Sigrid, Ma, Chengjie, Song, Renhua, Fuentes-Iglesias, Alejandro, Hassabi, Bouchra, Putmans, Pascale, Mies, Frédérique, Menschaert, Gerben, and Wong, Justin J. L.
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CELL differentiation ,RNA ,EMBRYONIC stem cells ,MESSENGER RNA ,MICE ,CYTOSINE - Abstract
Tet-enzyme-mediated 5-hydroxymethylation of cytosines in DNA plays a crucial role in mouse embryonic stem cells (ESCs). In RNA also, 5-hydroxymethylcytosine (5hmC) has recently been evidenced, but its physiological roles are still largely unknown. Here we show the contribution and function of this mark in mouse ESCs and differentiating embryoid bodies. Transcriptome-wide mapping in ESCs reveals hundreds of messenger RNAs marked by 5hmC at sites characterized by a defined unique consensus sequence and particular features. During differentiation a large number of transcripts, including many encoding key pluripotency-related factors (such as Eed and Jarid2), show decreased cytosine hydroxymethylation. Using Tet-knockout ESCs, we find Tet enzymes to be partly responsible for deposition of 5hmC in mRNA. A transcriptome-wide search further reveals mRNA targets to which Tet1 and Tet2 bind, at sites showing a topology similar to that of 5hmC sites. Tet-mediated RNA hydroxymethylation is found to reduce the stability of crucial pluripotency-promoting transcripts. We propose that RNA cytosine 5-hydroxymethylation by Tets is a mark of transcriptome flexibility, inextricably linked to the balance between pluripotency and lineage commitment. TET mediated RNA-hydroxymethylation (5hmC) has been detected in mammals, but its physiological role remains unclear. Here the authors map 5hmC during embryonic stem cell (ESC) differentiation and find that Tet-mediated RNA hydroxymethylation reduces the stability of crucial pluripotency related transcripts. [ABSTRACT FROM AUTHOR]
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- 2020
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11. Challenges in defining the role of intron retention in normal biology and disease.
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Vanichkina, Darya P., Schmitz, Ulf, Wong, Justin J.-L., and Rasko, John E.J.
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CELL proliferation , *GENETIC regulation , *MESSENGER RNA , *GENETIC engineering , *EXONS (Genetics) - Abstract
RNA sequencing has revealed a striking diversity in transcriptomic complexity, to which alternative splicing is a major contributor. Intron retention (IR) is a conserved form of alternative splicing that was originally overlooked in normal mammalian physiology and development, due mostly to difficulties in its detection. IR has recently been revealed as an independent mechanism of controlling and enhancing the complexity of gene expression. IR facilitates rapid responses to biological stimuli, is involved in disease pathogenesis, and can generate novel protein isoforms. Many challenges, however, remain in detecting and quantifying retained introns and in determining their effects on cellular phenotype. In this review, we provide an overview of these challenges, and highlight approaches that can be used to address them. [ABSTRACT FROM AUTHOR]
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- 2018
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12. A dynamic intron retention program in the mammalian megakaryocyte and erythrocyte lineages.
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Edwards, Christopher R., Ritchie, William, Wong, Justin J.-L., Schmitz, Ulf, Middleton, Robert, Xiuli An, Mohandas, Narla, Rasko, John E. J., and Blobel, Gerd A.
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INTRONS , *MAMMALS , *MEGAKARYOCYTES , *ERYTHROCYTES , *ALTERNATIVE RNA splicing , *MESSENGER RNA , *GRANULOCYTES , *HEMATOPOIETIC growth factors - Abstract
Intron retention (IR) is a form of alternative splicing that can affect mRNA levels through nonsense-mediated decay or by nuclear mRNA detention. A complex, dynamic IR pattern has been described in maturing mammalian granulocytes, but it is unknown whether IR occurs broadly in other hematopoietic lineages. We globally assessed IR in primary maturing mammalian erythroid and megakaryocyte (MK) lineages as well as their common progenitor cells (MEPs). Both lineages exhibit an extensive differential IR program involving hundreds of introns and genes with an overwhelming loss of IR in erythroid cells and MKs compared with MEPs. Moreover, complex IR patterns were seen throughout murine erythroid maturation. Similarly complex patterns were observed in human erythroid differentiation, but not involving the murine orthologous introns or genes. Despite the common origin of erythroid cells and MKs, and overlapping gene expression patterns, the MK IR program is entirely distinct from that of the erythroid lineage with regards to introns, genes, and affected gene ontologies. Importantly, our results suggest that IR serves to broadly regulate mRNA levels. These findings highlight the importance of this understudied form of alternative splicing in gene regulation and provide a useful resource for studies on gene expression in the MK and erythroid lineages. [ABSTRACT FROM AUTHOR]
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- 2016
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