1. Partial characterization of deltamethrin metabolism catalyzed by chymotrypsin
- Author
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Yang, Qinggui, Sun, Lixin, Zhang, Donghui, Qian, Jin, Sun, Yan, Ma, Lei, Sun, Jing, Hu, Xiaobang, Tan, Wenbin, Wang, Weijie, and Zhu, Changliang
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TOXICOLOGY of insecticides , *METABOLITES , *CHYMOTRYPSIN , *SPECTROPHOTOMETRY , *PANCREAS , *GAS chromatography , *MASS spectrometry - Abstract
Abstract: Deltamethrin degradation was assessed by measuring deltamethrin reduction with GC–MS and UV–Vis spectrophotometry following incubation of various concentrations (4.96–24.80μM) of deltamethrin with α-chymotrypsin from bovine pancreas. The retention times of crude products of deltamethrin metabolic reactions were 37.968min, 37.415min, 36.490min, and 35.895min. The UV–Vis spectrophotometric peak absorbance of deltamethrin was at 264nm, and the peak absorbance of deltamethrin metabolic products were at 250nm and 296nm, respectively. Michaelis–Menten metabolic rate constants (V max and K m) were calculated by nonlinear regression for chymotrypsin using GraphPad Prism 4.0, the V max was 97.97±26.57nmol/L/min and K m was 7.84±3.83μM. The larvicidal bioassay tests indicated that the mixture from the degradation reaction showed LC50 increased significantly (P <0.05). This is the first report demonstrating deltamethrin metabolism by chymotrypsin. [Copyright &y& Elsevier]
- Published
- 2008
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