1. Altered post-mortem metabolism identified in very fast chilled lamb M. longissimus thoracis et lumborum using metabolomic analysis.
- Author
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Warner RD, Jacob RH, Rosenvold K, Rochfort S, Trenerry C, Plozza T, and McDonagh MB
- Subjects
- Adenosine metabolism, Animals, Chromatography, High Pressure Liquid, Food Handling, Hydrogen-Ion Concentration, Magnetic Resonance Spectroscopy, Phosphocreatine metabolism, Sarcomeres chemistry, Sheep, Domestic, Tandem Mass Spectrometry, Cold Temperature, Meat analysis, Metabolomics, Muscle, Skeletal chemistry, Postmortem Changes
- Abstract
The aim of this experiment was to use metabolomic techniques to investigate the energy metabolism in lamb M. longissimus thoracis et lumborum subjected to very fast chilling (VFC) post-mortem. The tissue was prepared by 2 different operators and subjected to very fast chilling (less than 0°C within 1.5h of slaughter) or typical chilling regimes (Control; 0°C within 22h of slaughter). Non-targeted metabolomic analysis ((1)H NMR) and targeted analysis ((31)P NMR, HPLC-PDA and HPLC-MS/MS) were used to examine the change in muscle metabolites post-mortem. One VFC treatment, which resulted in a colder core temperature and more tender meat, had higher levels of glycolytic intermediate metabolites pre-rigor as well as more of the end-products of adenosine and nicotine nucleotide metabolism pre-rigor, relative to conventionally chilled treatments. In conclusion, VFC to less than 0°C within 1.5h of slaughter causes considerable changes in metabolism and rigor onset, which are associated with tender meat., (Crown Copyright © 2015. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2015
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