1. Metagenomic bacterial community profiles of chicken embryo gastrointestinal tract by using T-RFLP analysis.
- Author
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Ilina LA, Yildirim EA, Nikonov IN, Filippova VA, Laptev GY, Novikova NI, Grozina AA, Lenkova TN, Manukyan VA, Egorov IA, and Fisinin VI
- Subjects
- Amplified Fragment Length Polymorphism Analysis, Animals, Chick Embryo, Intestinal Mucosa metabolism, Rickettsieae genetics, Rickettsieae isolation & purification, Genome, Bacterial, Intestines microbiology, Metagenome, Microbiota
- Abstract
Thirty microbial phylotypes of microorganisms were found in the gastrointestinal tract of chicken belonging to the Hajseks White breed, and 38 phylotypes were found in the gastrointestinal tract of chicken belonging to the Hajseks Brown breed. The microbiome of the gastrointestinal tract of the chicken embryos of the Hajseks White breed was dominated by the typical representatives of avian intestinal microflora--bacteria of the family Enterobacteriaceae (47.3%), orders Actinomycetales (13.6%) and Bifidobacteriales (20.6%), and the family Lachnospiraceae (1.1%). The microbiome of the gastrointestinal tract of the chicken embryos of the Hajseks Brown breed was dominated by the pathogenic bacteria of the order Rickettsiales (94.8%). The metagenome of gastrointestinal tract of both breeds also contained a small number of genes of unidentified bacteria.
- Published
- 2016
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