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The article focuses on a clinical case involving a soft tissue lesion in the oral cavity, discussing topics like the diagnosis of oral metastasis from colorectal cancer, its rarity, and the treatment's success using targeted immunotherapy. It details the case history, diagnosis, treatment with pembrolizumab, and the positive response to therapy leading to complete regression of the oral metastasis, highlighting the significance of immune checkpoint blockade in managing such cases.

The article describes the case of a 53-year-old woman with T2N2Mx oropharyngeal squamous cell carcinoma who had been treated with concurrent chemotherapy and radiation and was found to have biopsy-confirmed adrenal gland metastasis of the squamous cell carcinoma. Clinical images of digital acrometastasis are also presented.

Circulating tumour cell (CTC)–derived organoids have the potential to provide a powerful tool for personalised cancer therapy but are restrained by low CTC numbers provided by blood samples. Here, we used diagnostic leukapheresis (DLA) to enrich CTCs from patients with metastatic prostate cancer (mPCa) and explored whether organoids provide a platform for ex vivo treatment modelling. We prospectively screened 102 patients with mPCa and performed DLA in 40 patients with ≥5 CTCs/7.5 mL blood. We enriched CTCs from DLA using white blood cell (WBC) depletion alone or combined with EpCAM selection. The enriched CTC samples were cultured in 3D to obtain organoids and used for downstream analyses. The DLA procedure resulted in a median yield of 5312 CTCs as compared with 22 CTCs in 7.5 mL of blood. Using WBC depletion, we recovered 46% of the CTCs, which reduced to 12% with subsequent EpCAM selection. From the isolated and enriched CTC samples, organoid expansion succeeded in 35%. Successful organoid cultures contained significantly higher CTC numbers at initiation. Moreover, we performed treatment modelling in one organoid cell line and identified substantial tumour heterogeneity in CTCs using single cell DNA sequencing. DLA is an efficient method to enrich CTCs, although the modest success rate of culturing CTCs precludes large scale clinical application. Our data do suggest that DLA and subsequent processing provides a rich source of viable tumour cells. Therefore, DLA offers a promising alternative to biopsy procedures to obtain sufficient number of tumour cells to study sequential samples in patients with mPCa. NL6019. • Leukapheresis offers 240-fold more circulating tumour cells (CTCs) than blood samples. • Using optimised methods, 46% of the CTCs were recovered. • Organoid expansion succeeded in 35% of the patient samples. • Substantial intra-patient heterogeneity was suggested by single cell DNA sequencing. • CTC derived organoid line reflected patient's treatment response. [ABSTRACT FROM AUTHOR]