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Accelerants can improve the anaerobic performance of a microbial electrolysis cell coupled anaerobic digestion (MEC-AD). MAX phase titanium aluminum carbide (MAX), multilayer Ti 3 C 2 T X MXene (ML-MXene) and few-layer Ti 3 C 2 T X MXene (FL-MXene) were utilized as accelerants for MEC-AD to promote CH 4 production and CO 2 reduction at a voltage of 0.6 V. The highest CH 4 yield (358.7 mL/g VS) and the lowest CO 2 yield (57.4 mL/g VS) relative to the control group (170.6 and 125.1 mL/g VS) were obtained in MEC-AD with ML-MXene (0.035 wt%). The digestates of MEC-AD with 0.035 wt% ML-MXene have superior thermal stability (40.9%) and total nutrient content (42.1 g/kg). The ML-MXene enhanced the abundances of Methanosarcina and Methanobacterium. This work highlights the possible role of MXene in promoting methanogenesis. These important findings provide a novel avenue for the development of MXene accelerants for MEC-AD systems., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Black phosphorus (BP) and BP modified by hydrogen peroxide (MBP) were used as accelerants to enhance CH 4 production and CO 2 reduction in microbial electrolysis cells (MECs) coupled with anaerobic co-digestion systems (MEC-AcoD). The MEC-AcoD group with a voltage of 0.6 V and 0.03 wt.% of MBP accelerant (MEC 0.6 MBP 0.03 ) had the largest CH 4 yield (242.1 mL/g VS) and the smallest carbon dioxide yield (97.6 mL/g VS) compared with the control group (141.2 mL/g VS, 146.9 mL/g VS). The digestates that used MEC 0.6 MBP 0.03 exhibited superior thermal stability (46.2 %) and total nutrient contents (44.5 g/kg). These improvements may be attributed to the superior electron exchange capacity and physicochemical properties of MBP. Herein, we propose a strategy to understand enhanced CH 4 production and CO 2 reduction in anaerobic co-digestion and MEC-AcoD systems using MBP accelerants. Notably, combining MBP and MEC could effectively promote anaerobic co-digestion performance., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Plenty of refractory and environmentally hazardous bag-filter gas dust (BGD) is produced in the iron-making process. The effects of untreated BGD on anaerobic digestion (AD) with cattle manure were investigated. The BGD had the potential to boost the methane yield and digestate utilization considerably. The digester with 2.0 wt% BGD gained the highest methane yield (256.3 mL/g VS) and chemical oxygen demand removal rate (56.8%), 51.3% and 20.1% higher than that (169.4 mL/g VS, 47.3%) of the control group, respectively. The digestates with BGD possessed a utilization potential with the stability of 49.5-57.9% and fertility of 4.65-4.86%. Electrochemical measurements demonstrated that 2.0 wt% BGD improved the electron transport capacity of the AD system and increased the limiting current and redox peak current by 40.3% and 12.9%, respectively. A strategy for understanding the BGD reinforcing methanogenesis was proposed. This work also provides a technical support for recycling the BGD., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

A series of surface-annealed titanium spheres (Ti-A, Ti-B, and Ti-C) in different atmospheres were used as accelerants in anaerobic co-digestion (AcoD) systems under magnetic field (MF). Surface-annealed titanium spheres and MF exhibit remarkable coupling and promoting effects on the AcoD performance. The cumulative biogas yield (435.84-552.60 mL/g VS) and total chemical oxygen demand (COD) degradation efficiency (59.76%-71.28%) of the AcoD systems with Ti MF , Ti-A MF , Ti-B MF , and Ti-C MF were significantly higher than control (357.66 mL/g VS and 51.5%). The digestates of the AcoD system with surface-annealed Ti spheres delivered excellent stability (49.83%-59.90%) and fertilizer (4.21%-4.56%). This work clarifies the possible role of surface-annealed Ti spheres in enhancing methanogenesis., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

The anaerobic-co-digestion (AcoD) of dairy-manure (DM) and aloe-peel-residue (ALR) waste is enhanced by combining static-magnetic-field (SMF) and fly-ash (FA). Varying SMF intensities (5-30 mT) were applied to the co-digestion digesters containing the optimum FA concentration (1.5 wt.%), which were selected from co-digestion systems with a varying FA (0-2 wt.%). All experimental groups exhibit the greater COD removal rates (51.56-64.19%) and cumulative biogas yields (604.14-671.64 mL/g VS) than reference group (37.77% and 433.19 mL/g VS). The digester with optimum FA concentration (1.5 wt.%) under 5 mT shows the highest biogas yield (671.64 mL/g VS), and exhibits superior digestate stability (45.4%) and fertility (7.01%) for fertilizer utilization. A powerful strategy for understanding the underlying mechanism of the SMF and FA accelerant in an enhanced AcoD system is proposed. This work documents an innovative technique for an enhanced AcoD system using the SMF coupled with FA accelerant., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Dry anaerobic digestion (AD) with high total solid content might upgrade conventional wet AD by enhancing digestion and reducing liquid digestate generation, which was not so popular due to lack of adequate knowledge and perceived operational complexity. In this study long term (447 days) pilot scale investigation was performed for various feedstocks including pig manure, corn straw and sludge. It was found dry method was more suitable for reactor start-up than wet method with relieved inhibition. Volatile fatty acid (VFA)/alkalinity (0.1 ~ 0.4) was effective as dry AD system stability indicator. Organic loading rate of 4.8 kg/(m 3 ·d) and temperature of 48 °C was optimized. Co-digestion achieved better volatile solid removal and system stability. Both hydrogenotrophic and acetoclastic methanogens stably existed in rector while hydrogenotrophic obtained higher abundance. This research contributed to understanding of reactor start-up, system stability indicator and optimized operational conditions (organic loading rate and temperature) during dry AD process., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Anaerobic digestion, a recently hot technology to produce biogases especially methane generation for biofuel from wastewater, is considered an effective explanation for energy crisis and global pollution threat. A complex microbiome population is present in sludge, which plays an important role in the digestion of complex polymer into simple monomers. 16S rRNA approaches simply are not enough for amplification due to the involvement of extreme complex population. However, Illumina sequencing is a recent powerful technology to reveal the entire microbiome structure and methane generation pathways in anaerobic digestion. Metagenomic sequencing was tested to reveal the microbial structure of a digested sludge from a local wastewater treatment plant in Beijing. The Illumina HiSeq program was used to extract about 5 GB of data for metagenomic analysis. The classification investigation revealed about 97.64% dominancy of bacteria while 1.78% were detected to be archaea using MG-RAST server. The most abundant bacterial communities were reported to be Actinobacteria, Bacteroidetes, Firmicutes and Proteobacteria. Furthermore, the important microbiome involved in methane generation was revealed. The dominant methanogens were detected (Methanosaeta and Methanosarcina), with affiliation of dominant genes involved in acetoclastic methanogenesis in a digesting sludge. The metagenomic analysis showed that microbial structure and methane generation pathways were successfully dissected in an anaerobic digester.

High solid anaerobic digestion (HSAD) is a rapidly developed anaerobic digestion technique for treating municipal sludge, and has been widely used in Europe and Asia. Recently, the enhanced HSAD process with thermal treatment showed its advantages in both methane production and VS reduction. However, the understanding of the microbial community is still poor. This study investigated microbial communities in a pilot enhanced two-stage HSAD system that degraded waste activated sludge at 9% solid content. The system employed process "thermal pre-treatment (TPT) at 70 °C, thermophilic anaerobic digestion (TAD), and mesophilic anaerobic digestion (MAD)". Hydrogenotrophic methanogens Methanothermobacter spp. dominated the system with relative abundance up to about 100% in both TAD and MAD. Syntrophic acetate oxidation (SAO) bacteria were discovered in TAD, and they converted acetate into H₂ and CO₂ to support hydrogenotrophic methanogenesis. The microbial composition and conversion route of this system are derived from the high solid content and protein content in raw sludge, as well as the operational conditions. This study could facilitate the understanding of the enhanced HSAD process, and is of academic and industrial importance., Competing Interests: The authors declare no conflict of interest.

Hydrogenotrophic methanogenesis has been proved to be a feasible biological method for biogas upgrading. To improve its performance, the feasibility of typical anaerobic granules as the inoculum was investigated in both batch and continuous experiments. The results from batch experiments showed that glucose-acclimated granules seemed to perform better than granules acclimated to acidified products (AP, i.e. acetate, propionate and ethanol) in in situ biogas upgrading systems and a slightly higher H2 consumption rate (1.5 mmol H2 g VSS(-1) h(-1)) was obtained for glucose-acclimated granules. For AP-acclimated granules, the inhibition on anaerobic digestion and pH increase (up to 9.55±0.16) took place, and the upgrading performance was adversely affected. In contrast, better performance for AP-acclimated granules was observed in ex situ systems, possibly due to their higher hydrogenotrophic methanogenic activities (HMA). Moreover, when gas-liquid mass transfer limitations were alleviated, the upgrading performance was significantly improved (three-fold) for both glucose-acclimated and AP-acclimated granules. The HMA of anaerobic granules could be further enhanced to improve biogas upgrading performance via continuous cultivation with H2/CO2 as the sole substrate. During the three months' cultivation, secondary granulation and microbial population shift were observed, but anaerobic granules still remained intact and their HMA increased from 0.2 to 0.6 g COD g VSS(-1) d(-1). It indicated that the formation of hydrogenotrophic methanogenic granules, a new type of anaerobic granules specialized for high-rate hydrogenotrophic methanogenesis and biogas upgrading, might be possible. Conclusively, anaerobic granules showed great potential for biogas upgrading.

A lab-scale continuously-stirred tank reactor (CSTR), used for anaerobic co-digestion of fruit and vegetable waste (FVW) and food waste (FW) at different mixture ratios, was operated for 178 days at the organic loading rate of 3 kg VS (volatile solids)/(m3 x day). The dynamics of the Archaeal community and the correlations between environmental variables and methanogenic community structure were analyzed by polymerase chain reactions--denaturing gradient gel electrophoresis (PCR-DGGE) and redundancy analysis (RDA), respectively. PCR-DGGE results demonstrated that the mixture ratio of FVW to FW altered the community composition of Archaea. As the FVW/FW ratio increased, Methanoculleus, Methanosaeta and Methanosarcina became the predominant methanogens in the community. Redundancy analysis results indicated that the shift of the methanogenic community was significantly correlated with the composition of acidogenic products and methane production yield. Different mixture ratios of substrates led to different compositions of intermediate metabolites, which may affect the methanogenic community. These results suggested that the analysis of microbial communities could be used to diagnose anaerobic processes.

Concentrating organic matter in sludge and converting it into methane through anaerobic bioconversion can improve resource recovery from domestic wastewater. Enhanced membrane coagulation (EMC) is highly efficient at concentrating organic matter, but residual coagulants (aluminum salts) can obstruct bioconversion by blocking microbial access. Limited research exists on evaluating EMC sludge bioconversion performance and addressing coagulant inhibition. This study proposes alkaline pre-fermentation to break down HO-Al-P backbones in coagulated sludge flocs, thereby improving hydrolysis and organic acid production for anaerobic digestion. Among the tested alkaline conditions (pH 9, pH 10, pH 11), pre-fermentation at pH 11 released the most organic matter (4710.0 mg/L SCOD), 20.4 times higher than without alkaline treatment. At pH 11, phosphate (61 mg/L PO43−–P) and organic acid production (2728.1 mg COD/L, with nearly 50% acetic acid) peaked, resulting in superior volatile solids removal (65.2%) and methane production (185.8 mL/g VS) during anaerobic digestion. Alkaline pre-fermentation favored alkali-tolerant bacteria such as Firmicutes and Actinobacteria, especially at pH 11, while neutrophilic Proteobacteria were suppressed. Trichococcus and Bifidobacterium, known acid producers, dominated under all conditions, with their abundance increasing at higher pH levels. Anaerobic digestion enriched fermentative bacteria like Chloroflexi and Synergistota (e.g., Thermovirga), especially in high pH reactors. Methanothrix, an acetoclastic methanogen, became the dominant methanogenic archaeon, indicating that methane production from EMC sludge primarily followed the acetoclastic methanogenesis pathway. Our findings demonstrate that alkaline pre-fermentation at pH 11 significantly enhances the hydrolysis efficiency of EMC sludge for methane recovery. [ABSTRACT FROM AUTHOR]

This study investigated the efficiency of biogas production from sewage concentrate through anaerobic digestion. A continuous stirred tank reactor with a 900-mL working volume was used. The experiment was designed to investigate the influence of organic loading rate on the efficiency of biogas production and to determine the most suitable organic loading rate condition for methane production from sewage concentrate by using continuous stirred tank reactor. The reactor was operated at different organic loading rates of 1.8, 0.8, and 0.6 gCOD/(L.d). The methane composition of the biogas produced from the treatment organic loading rate (OLR). The beginning of the experiment recorded low methane production because of the high organic loading rate. However, the later part of the experiment recorded high and stable biogas production because of the relatively low OLR. Results suggested that a 0.6 gCOD/(L.d) OLR was the most efficient setup parameter for ideal methane production from sewage concentrate by using continuous stirred tank reactor. [ABSTRACT FROM AUTHOR]

Highlights • An EGSB reactor was operated at 9.96 kg COD/(m3·d) with COD removal efficiency of 72%. • Methane content range was 70–92% with an average methane content of 84%. • Methanobacterium and Methanomassiliicoccus were dominant archaea in granular sludge. • Hydrogenotrophic and methylotrophic types were the main methanogenic pathway. Abstract In this study, the anaerobic treatment and microbial characteristics of high-concentration cephalosporin wastewater were studied. A pilot-scale expanded granular sludge bed (EGSB) reactor was designed to treat cephalosporin wastewater, whose diameter, height and effective volume were 0.5 m, 4.9 m, 0.92 m3, respectively. With mixed high-concentration cephalosporin wastewater and municipal wastewater as a substrate, the anaerobic reactor was started and operated 414 days. An average COD removal efficiency of 72% was achieved at an organic loading rate (OLR) of 9.96 kg COD/(m3·d), with a hydraulic retention time (HRT) of 25 h. The average methane content reached 82%. Methanobacterium and Methanomassiliicoccus were predominant archaea in the granular sludge for each of the organic loading rates, and the predominant methane-producing pathway was hydrogenotroph and methylotroph. Those results demonstrated that the EGSB reactor could treat high-concentration cephalosporin wastewater with a unique methane-producing pathway. [ABSTRACT FROM AUTHOR]

The feasibility of corn stalk anaerobic digestion was investigated in an upflow anaerobic solid-state (UASS) reactor with an additional anaerobic filter (AF) reactor. One single-stage (UASS) and two two-stage systems (UASS + AF) were compared at organic loading rates (OLR) of 2.5, 4.5, and 8.0 g vs L −1 d −1 . The single-stage system was operated under mesophilic conditions and the two-stage systems under both mesophilic and thermophilic conditions. Independently of OLR, the mesophilic two-stage system showed higher average methane production rates (0.50 ± 0.01, 0.63 ± 0.11, and 0.67 ± 0.12 L CH 4 L - 1 d - 1 ) than the single-stage (0.46 ± 0.02, 0.46 ± 0.06, and 0.31 ± 0.09 L CH 4 L - 1 d - 1 ) and thermophilic two-stage (0.30 ± 0.02, 0.42 ± 0.05, and 0.66 ± 0.12 L CH 4 L - 1 d - 1 ) systems. Respective methane yields were 84–201, 39–184, and 88–119 mL g vs −1 . The observed advantage of mesophilic digestion is of particular interest, as degradation of lignocellulosic biomass is usually reported to benefit from higher temperature. The results show the general feasibility of an additional AF reactor after the UASS digestion of corn stalk under mesophilic conditions. [ABSTRACT FROM AUTHOR]

This paper presents a potential process that can enhance H 2 transformation to CH 4 and simultaneously upgrading biogas by using hydrogenotrophic methanogens. For the first time, anaerobic granules were developed in upflow anaerobic sludge blanket (UASB) reactor feeding H 2 /CO 2 syngas as the sole substrate and the granule characterization was thoroughly investigated. The results from experiment revealed that the H 2 consumption rates of UASB reactor increased from 32.2 mmol L−1·d−1 at H 2 feeding rate 0.08 g L−1·d−1 to 132.0 mmol L−1·d−1 at 0.37 g L−1·d−1, indicating that the hydrogenotrophic methanogenesis pathway was stimulated by injection of H 2. Abundant cavities and cracks were observed on the surface and cross-section of granules, which greatly facilitated internally transferring H 2 /CO 2 synthesis gas and biogas escape. The abundance of hydrogenotrophic Methanobacterium increased, while Methanosaeta , Methanosarcina , and Methanomassiliicoccus decreased with increasing H 2 feeding rate. In general, this paper offers a feasible solution in terms of energy transformation and connecting power to fuel. [Display omitted] • Cultivating hydrogenotrophic methanogenic granule by feeding H 2 /CO 2 syngas was feasible. • Hydrogenotrophic pathway of granule sludge could be attributed to Methanobacterium. • Granule converted H 2 /CO 2 into CH 4 in one-step which lead to simple uniform microstructure. • Sludge cultivated with H 2 have advantageous effect on the growth of Methanobacterium. [ABSTRACT FROM AUTHOR]