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The goal of this study was to validate the analytical technique for determining the immediate development of lead (Pb), cadmium (Cd), mercury (Hg), and arsenic (As) in various Indian seafood products. According to Commission Regulation (EC) 333/2007, various marine foods, including crustaceans, cephalopods, and fish species, were employed for the validation of the developed method by ICP-MS. HNO3 and H2O2 were combined to prepare the sample during microwave digestion. Specificity/selectivity, linearity, LOD, LOQ, precision-repeatability and reproducibility, accuracy-recovery, robustness, and fitness studies were used to validate the approach. The maximum RSD value and Horrat value (HorRat) for the within-lab reproducibility for all analytes (Pb, Cd, Hg, and As) in marine food were 5% and 1 respectively. The mean recovery for all analytes examined at three spiking levels (0.5, 1 & 1.5 of the permitted limit) was between 92.67 and 107.33%.Whereas limit of detection (LOD) values for Pb, Cd, Hg and As were 0.018 μg/g, 0.032 μg/g, 0.031 μg/g and 0.034 mg/kg for repeatability 6% and <1 respectively. 0.061 μg/g, 0.127 μg/g, 0.0.103 μg/g and 0.101 μg/g respectively, were the limit of quantitation (LOQ) values. At a 95% confidence level, the method's relative extended measurement uncertainty (k=2) was 9%. In fact, the developed method's precision was examined by taking part in LGC proficiency testing (round 253, sample 742); the outcomes (Z score, i.e. < 1) showed that this analytical method could be used for the routine analysis of these four toxic metals in seafood with acceptable analytical performance in the laboratory. [ABSTRACT FROM AUTHOR]

A simple and inexpensive method was developed using high performance liquid chromatography with PDA detection for determination of granisetron hydrochloride and related impurities (2-methyl-N- [(1R,3r,5S)-9-methyl-9-azabicyclo[3.3.1]non-3-yl]-2H-indazole-3-carboxamide, 1-Methyl-N-[(1R,3r,5S)- 9-methyl-9-azabicyclo[3.3.1]non-3-yl]-1H-indazole-3-carboxamide hydrochloride, N-[(1R,3r,5S)-9-azabicyclo[3.3.1]non-3-yl]-1-methyl-1H-indazole-3-carboxamide and 1-methyl-1H-indazole-3-carboxylic acid. The chromatographic separations were achieved on (250×4.6 mm), 5.0 µm make: Waters, Symmetry Shield C8 column employing acetonitrile: 2% V/V H3PO4 in MilliQ water + 0.1% V/V hexylamine in water, 800:200 V/V (pH adjusted to 7.5 using triethylamine) mobile phase with isocratic programme. A flow rate of 1.5 mL/min was chosen. Four impurities were eluted within 20 minutes. The column temperature was maintained at 40°C and a detector wavelength of 300 nm was employed. The method was successfully validated by establishing system suitability, specificity, linearity, accuracy, limit of detection and limit of quantification. [ABSTRACT FROM AUTHOR]