1. Association between Exosomal miRNAs and Coronary Artery Disease by Next-Generation Sequencing.
- Author
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Chang, Sheng-Nan, Chen, Jien-Jiun, Wu, Jo-Hsuan, Chung, Yao-Te, Chen, Jin-Wun, Chiu, Chu-Hsuan, Liu, Chia-Ju, Liu, Meng-Tsun, Chang, Yi-Cheng, Li, Chin, Lin, Jou-Wei, Hwang, Juey-Jen, and Lien, Wen-Pin
- Subjects
CORONARY artery disease ,NUCLEOTIDE sequencing ,MICRORNA ,EXOSOMES ,CORONARY arteries - Abstract
Background: Among various bio-informative molecules transferred by exosomes between cells, micro RNAs (miRNAs), which remain remarkably stable even after freeze-and-thaw cycles, are excellent candidates for potential biomarkers for coronary artery disease (CAD). Methods: Blood samples were collected from the coronary arteries of 214 patients diagnosed with three-vessel CAD and 140 without CAD. After precipitation extraction, the amounts of exosomes were found to decrease with increased age and three-vessel CAD. Next-generation sequencing was performed to further explore the possible relationship between exosomal miRNAs and CAD. Results: Eight exosomal miRNAs showed altered expression associated with CAD. The up-regulated miRNAs in CAD were miRNA-382-3p, miRNA-432-5p, miRNA-200a-3p, and miRNA-3613-3p. The down-regulated miRNAs were miRNA-125a-5p, miRNA-185-5p, miRNA-151a-3p, and miRNA-328-3p. Conclusion: We successfully demonstrated particular exosomal miRNAs that may serve as future biomarkers for CAD. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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