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Summary: What microbiology beholds after a decade and a half in the future requires a vision based on the facts and ongoing trends in research and technological advancements. While the latter, assisted by microbial dark matter, presents a greater potential of creating an upsurge in in‐situ and ex‐situ rapid microbial detection techniques, this anticipated change will also set forth a revolution in microbial cultivation and diversity analyses. The availability of a microbial genetic toolbox at the expanse will help complement the current understanding of the microbiome and assist in real‐time monitoring of the dynamics for detecting the health status of the host with utmost precision. Alongside, in light of the emerging infectious diseases, antimicrobial resistance (AMR) and social demands for safer and better health care alternatives, microbiology laboratories are prospected to drift in terms of the volume and nature of research and outcomes. With today's microbiological lens, one can predict with certainty that in the years to come, microbes will play a significant role in therapeutic treatment and the designing of novel diagnostic techniques. Another area where the scope of microbial application seems to be promising is the use of novel probiotics as a method to offer health benefits whilst promoting metabolic outputs specific for microbiome replenishment. Nonetheless, the evolution of extraterrestrial microbes or the adaptation of earth microbes as extraterrestrial residents are also yet another prominent microbial event one may witness in the upcoming years. But like the two sides of the coin, there is also an urgent need to dampen the bloom of urbanization, overpopulation and global trade and adopting sustainable approaches to control the recurrence of epidemics and pandemics. [ABSTRACT FROM AUTHOR]

4627950 23 Kalia VC, Kumar P. Genome wide search for biomarkers to diagnose Yersinia infections. 1:CAS:528:DC%2BB3cXnsV2mtrY%3D 7 Kumar P, Singh M, Mehariya S, Patel SKS, Lee JK, Kalia VC. 1:STN:280:DC%2BC2MjjsFGitA%3D%3D 22 Kalia VC, Kumar P, Kumar R, Mishra A, Koul S. Genome wide analysis for rapid identification of Vibrio species. 1:CAS:528:DC%2BC2cXmvVWiurk%3D 8 Kumar P, Mehariya S, Ray S, Mishra A, Kalia VC. [Extracted from the article]

Background and Aim: Although the gut microbiome of patients with ulcerative colitis (UC) has been characterized, no study has characterized the gut microbiome in acute severe colitis (ASC). We compared the gut microbiome of patients with UC, ASC, and healthy controls (HCs). Methods: Patients with mild to moderate UC (n = 24), ASC (n = 19 with 21 episodes) and HCs (n = 50) were recruited prospectively. A 16SrDNA amplicon approach was used to explore gut microbial diversity and taxonomic repertoires. UC was diagnosed using European Crohn's and Colitis Organization guidelines, and ASC was diagnosed using Truelove and Witts' criteria. Results: The normalized alpha diversity was significantly lower in ASC than mild–moderately active UC (P < 0.05) or HC (P < 0.001). The gut microbiome in ASC was highly unstable, as characterized by high intracohort variation (analyzed using J‐divergence measure), which was significantly greater than in UC or HC. On principal coordinate analysis, the microbiome of HC and UC were similar, with the ASC cohort being distinct from both. Comparison of ranked abundances identified four distinct clusters of genera (G1, G2, G3, and G4), with specific trends in their abundance across three groups: G1/G2A clusters had the least, whereas G3 had the highest abundance in the ASC cohort. Conclusions: Gut microbial diversity is lower in ASC than mild–moderate UC or HCs. Gut microbiome composition is increasingly unstable in ASC, with a distinct abundance of specific genera varying between HCs and ASC. Mild–moderate UC lies within the spectrum. [ABSTRACT FROM AUTHOR]

The diversity of microbes and their pervasive influence on human beings is now well established. In contrast to global efforts on microbiome research, contributions from Indian R&D organizations are rather scarce. Efforts should be made to boost the microbiome research in India to harvest the fruits of the unique microbial diversity and use it for developing a sustainable economy. [Extracted from the article]

The Najafgarh drain plays a significant role in the pollution of the Yamuna River, accounting for 40% of the total pollution. Therefore, it is crucial to investigate and analyze the microbial diversity, metabolic functional capacity, and antibiotic resistance genes (ARGs) present in the Najafgarh drain. Additionally, studying the water quality and its relationship with the proliferation of microorganisms in the drain is of utmost importance. Results obtained confirmed the deteriorated water quality as physico-chemical parameters such as biochemical oxygen demand (BOD), chemical oxygen demand (COD), dissolved oxygen (DO), and total suspended solids (TSS) in the range of 125–140, 400–460, 0–0.2, 25–140.4 mg/l respectively violated the standard permissible national and global standards. In addition, the next generation sequencing (NGS) analysis confirm the presence of genus such as Thauera , Arcobacter , Pseudomonas , Geobacter , Dechloromonas , Tolumonas , Sulfurospirullum , Desulfovibrio , Aeromonas , Bacteroides , Prevotella , Cloacibacterium , Bifidobacterium , Clostridium etc. along with 864 ARGs in the wastewater obtained from the Najafgarh drain. Findings confirm that the pathogenic species reported from this dataset possess severe detrimental impact on faunal and human health. Further, Pearson's r correlation analysis indicated that environmental variables, mainly total dissolved solids (TDS) and chemical oxygen demand (COD), play a pivotal role in driving microbial community structure of this heavily polluted drain. Thus, the poor water quality, presence of a microbial nexus, pathogenic markers, and ARGs throughout this drain confirmed that it would be one potential contributor to the dissemination of disease-causing agents (pathogens) to the household and drinking water supplies in the near future. [Display omitted] • Physico-chemical analysis revealed deteriorated water quality and hypoxic condition. • COD, TSS, BOD, DO violated the standard permissible limit. • WGS analysis revealed that 41 genera and 69 pathogenic species were present in ND. • Genera such as Thauera , Arcobacter , Geobacter , etc., dominated the bacterial diversity. • Najafgarh drain plays significant role in the dissemination of ARGs across the metropolitan city, Delhi. [ABSTRACT FROM AUTHOR]