Search

Your search keyword '"Sannio F"' showing total 2 results
Start Over Author "Sannio F" Topic microbial sensitivity tests
2 results on '"Sannio F"'

1. Chaetomorpha linum Extract as a Source of Antimicrobial Compounds: A Circular Bioeconomy Approach.

Author

Barletta R, Trezza A, Geminiani M, Frusciante L, Olmastroni T, Sannio F, Docquier JD, and Santucci A

Subjects
Plant Extracts pharmacology, Plant Extracts chemistry, Palmitic Acid, Pseudomonas aeruginosa drug effects, Anti-Infective Agents pharmacology, Anti-Infective Agents chemistry, Enterococcus faecalis drug effects, Staphylococcus aureus drug effects, Chlorophyta, Seaweed, Microbial Sensitivity Tests, Anti-Bacterial Agents pharmacology, Anti-Bacterial Agents chemistry, Anti-Bacterial Agents isolation & purification
Abstract

The circular bioeconomy is currently a promising model for repurposing natural sources; these sources include plants due to their abundance of bioactive compounds. This study evaluated the antimicrobial properties of a Chaetomorpha linum extract. Chaetomorpha linum is an invasive macroalga from the Orbetello Lagoon (Tuscany, Italy), which grows in nutrient-rich environments and has been forming extended mats since 2005. The biomass is mechanically harvested and treated as waste, consuming considerable manpower and financial resources. As a potential way to increase the value of such waste, this study found that C. linum extract (CLE) is a source of antimicrobial compounds. The phytochemical characterization of the extract revealed the predominant presence of palmitic acid, a fatty acid with known antimicrobial activity. Based on such findings, four bacterial species of high clinical relevance ( Enterococcus faecalis , Staphylococcus aureus , Pseudomonas aeruginosa , and Escherichia coli ) were tested, revealing a notable antibacterial activity of the extract on Enterococcus faecalis (MIC, 32 μg/mL). Computational analyses identified a potential Enterococcus faecalis molecular target for palmitic acid, offering molecular insights on the interaction. This study presents a comprehensive in vitro and in silico approach for drug and target discovery studies by repurposing C. linum as a source of antimicrobial bioactive compounds.

Published
2024
Full Text
View/download PDF

2. Virtual screening identifies broad-spectrum β-lactamase inhibitors with activity on clinically relevant serine- and metallo-carbapenemases

Author

Spyrakis, F., Santucci, M., Maso, L., Cross, S., Gianquinto, E., Sannio, F., Verdirosa, F., De Luca, F., Docquier, J. D., Cendron, L., Tondi, D., Venturelli, A., Cruciani, G., and Costi, M. P.

Subjects
Virtual screening, Protein Conformation, Tetrazoles, lcsh:Medicine, Microbial Sensitivity Tests, Crystallography, X-Ray, Ligands, Article, beta-Lactamases, Databases, Bacterial Proteins, Anti-Bacterial Agents, Databases, Protein, Drug Design, Drug Discovery, Escherichia coli, Hydrolysis, Molecular Docking Simulation, Protein Binding, Recombinant Proteins, Semicarbazides, Serine, Sulfhydryl Compounds, Sulfonamides, beta-Lactamase Inhibitors, polycyclic compounds, lcsh:Science, Crystallography, Drug discovery, Protein, lcsh:R, biochemical phenomena, metabolism, and nutrition, bacterial infections and mycoses, X-Ray, lcsh:Q
Abstract

Bacteria are known to evade β-lactam antibiotic action by producing β-lactamases (BLs), including carbapenemases, which are able to hydrolyze nearly all available β-lactams. The production of BLs represents one of the best known and most targeted mechanisms of resistance in bacteria. We have performed the parallel screening of commercially available compounds against a panel of clinically relevant BLs: class A CTX-M-15 and KPC-2, subclass B1 NDM-1 and VIM-2 MBLs, and the class C P. aeruginosa AmpC. The results show that all BLs prefer scaffolds having electron pair donors: KPC-2 is preferentially inhibited by sulfonamide and tetrazole-based derivatives, NDM-1 by compounds bearing a thiol, a thiosemicarbazide or thiosemicarbazone moiety, while VIM-2 by triazole-containing molecules. Few broad-spectrum BLs inhibitors were identified; among these, compound 40 potentiates imipenem activity against an NDM-1-producing E. coli clinical strain. The binary complexes of the two most promising compounds binding NDM-1 and VIM-2 were obtained at high resolution, providing strong insights to improve molecular docking simulations, especially regarding the interaction of MBLs with inhibitors.

Published
2020
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results