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Your search keyword '"Fernanda Pessi de Abreu"' showing total 2 results
Start Over Author "Fernanda Pessi de Abreu" Topic microbiology
2 results on '"Fernanda Pessi de Abreu"'

1. Gene Regulatory Networks of Penicillium echinulatum 2HH and Penicillium oxalicum 114-2 Inferred by a Computational Biology Approach

Author

Alexandre Rafael Lenz, Edgardo Galán-Vásquez, Eduardo Balbinot, Fernanda Pessi de Abreu, Nikael Souza de Oliveira, Letícia Osório da Rosa, Scheila de Avila e Silva, Marli Camassola, Aldo José Pinheiro Dillon, and Ernesto Perez-Rueda

Subjects
Penicillium, regulatory network, orthologous, gene regulation, genomics, fungi, Microbiology, QR1-502
Abstract

Penicillium echinulatum 2HH and Penicillium oxalicum 114-2 are well-known cellulase fungal producers. However, few studies addressing global mechanisms for gene regulation of these two important organisms are available so far. A recent finding that the 2HH wild-type is closely related to P. oxalicum leads to a combined study of these two species. Firstly, we provide a global gene regulatory network for P. echinulatum 2HH and P. oxalicum 114-2, based on TF-TG orthology relationships, considering three related species with well-known regulatory interactions combined with TFBSs prediction. The network was then analyzed in terms of topology, identifying TFs as hubs, and modules. Based on this approach, we explore numerous identified modules, such as the expression of cellulolytic and xylanolytic systems, where XlnR plays a key role in positive regulation of the xylanolytic system. It also regulates positively the cellulolytic system by acting indirectly through the cellodextrin induction system. This remarkable finding suggests that the XlnR-dependent cellulolytic and xylanolytic regulatory systems are probably conserved in both P. echinulatum and P. oxalicum. Finally, we explore the functional congruency on the genes clustered in terms of communities, where the genes related to cellular nitrogen, compound metabolic process and macromolecule metabolic process were the most abundant. Therefore, our approach allows us to confer a degree of accuracy regarding the existence of each inferred interaction.

Published
2020
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2. Taxonomy, comparative genomics and evolutionary insights of Penicillium ucsense: a novel species in series Oxalica

Author

Alexandre Rafael Lenz, Eduardo Balbinot, Fernanda Pessi de Abreu, Nikael Souza de Oliveira, Roselei Claudete Fontana, Scheila de Avila e Silva, Myung Soo Park, Young Woon Lim, Jos Houbraken, Marli Camassola, Aldo José Pinheiro Dillon, Westerdijk Fungal Biodiversity Institute, and Westerdijk Fungal Biodiversity Institute - Food and Indoor Mycology

Subjects
Melanins, Cellulase/genetics, Cellulase, Melanins/metabolism, Penicillium/genetics, Penicillium, Genomics, General Medicine, Molecular Biology, Microbiology, Phylogeny
Abstract

The genomes of two Penicillium strains were sequenced and studied in this study: strain 2HH was isolated from the digestive tract of Anobium punctatum beetle larva in 1979 and the cellulase hypersecretory strain S1M29, derived from strain 2HH by a long-term mutagenesis process. With these data, the strains were reclassified and insight is obtained on molecular features related to cellulase hyperproduction and the albino phenotype of the mutant. Both strains were previously identified as Penicillium echinulatum and this investigation indicated that these should be reclassified. Phylogenetic and phenotype data showed that these strains represent a new Penicillium species in series Oxalica, for which the name Penicillium ucsense is proposed here. Six additional strains (SFC101850, SFCP10873, SFCP10886, SFCP10931, SFCP10932 and SFCP10933) collected from the marine environment in the Republic of Korea were also classified as this species, indicating a worldwide distribution of this new taxon. Compared to the closely related strain Penicillium oxalicum 114-2, the composition of cell wall-associated proteins of P. ucsense 2HH shows five fewer chitinases, considerable differences in the number of proteins related to β-D-glucan metabolism. The genomic comparison of 2HH and S1M29 highlighted single amino-acid substitutions in two major proteins (BGL2 and FlbA) that can be associated with the hyperproduction of cellulases. The study of melanin pathways shows that the S1M29 albino phenotype resulted from a single amino-acid substitution in the enzyme ALB1, a precursor of the 1,8-dihydroxynaphthalene (DHN)-melanin biosynthesis. Our study provides important knowledge towards understanding species distribution, molecular mechanisms, melanin production and cell wall biosynthesis of this new Penicillium species.

Published
2022

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