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Your search keyword '"broad-spectrum antimicrobial activity"' showing total 3 results
Start Over Descriptor "broad-spectrum antimicrobial activity" Topic microbiology
3 results on '"broad-spectrum antimicrobial activity"'

1. Endophytic Streptomyces sp. NEAU-ZSY13 from the leaf of Perilla frutescens, as a promising broad-spectrum biocontrol agent against soil-borne diseases

Author

Zhiyan Wang, Congting Gao, Jingquan Yang, Rui Du, Fanli Zeng, Hui Bing, Banghua Xia, Yue Shen, and Chongxi Liu

Subjects
soil-borne diseases, endophytic actinobacteria, broad-spectrum antimicrobial activity, biocontrol, niphimycin, Microbiology, QR1-502
Abstract

Soil-borne diseases cause significant economic losses in global agricultural production. These diseases are challenging to control due to the invasion of multiple pathogens into host plants, and traditional chemical control methods often yield unsatisfactory results. In this study, we isolated and identified an endophytic Streptomyces, designated as NEAU-ZSY13, from the leaf of Perilla frutescens. This isolate exhibited broad-spectrum antifungal activity against 17 soil-borne phytopathogenic fungi, with Bipolaris sorokiniana being the most prominent. Additionally, it displayed strong antibacterial activity against the soil-borne phytopathogenic bacterium Ralstonia solanacearum. To assess its biocontrol potential, the isolate was utilized to produce a biofertilizer through solid-state fermentation. The fermentation conditions were optimized using response surface methodology to maximize the spore production. The results revealed that more abundant spores were produced with a 1:2 ratio of vermicompost to wheat bran, 60% water content, 20% inoculation amount and 28°C. Subsequent pot experiments demonstrated that the application of the biofertilizer with a spore concentration of 108 CFU/g soil effectively suppressed the occurrence of tomato bacterial wilt caused by R. solanacearum and wheat root rot caused by B. sorokiniana, with biocontrol efficacies of 72.2 and 78.3%, respectively. Chemical analysis of NEAU-ZSY13 extracts, using nuclear magnetic resonance spectrometry and mass analysis, identified niphimycin C and niphimycin A as the primary active constituents. These compounds exhibited high activity against R. solanacearum (EC50 of 3.6 and 2.4 μg mL−1) and B. sorokiniana (EC50 of 3.9 and 3.4 μg mL−1). In conclusion, this study demonstrates the potential of Streptomyces sp. NEAU-ZSY13 as a biofertilizer for the control of soil-borne diseases.

Published
2023
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2. Novel water-based antiseptic lotion demonstrates rapid, broad-spectrum kill compared with alcohol antiseptic

Author

Colette Cozean, Jesse Cozean, and Steven E. Czerwinski

Subjects
Time Factors, medicine.drug_class, Alcohol, Bacterial Physiological Phenomena, medicine.disease_cause, Hand sanitizer, Benzethonium chloride, lcsh:Infectious and parasitic diseases, Microbiology, Persistence, chemistry.chemical_compound, Antiseptic, Humans, Medicine, lcsh:RC109-216, Microbial Viability, Bacteria, Traditional medicine, business.industry, lcsh:Public aspects of medicine, Fungi, Public Health, Environmental and Occupational Health, Water, lcsh:RA1-1270, General Medicine, Antimicrobial, Water based, Broad-spectrum antimicrobial activity, Infectious Diseases, chemistry, Alcohols, Lotion, Anti-Infective Agents, Local, Solvents, Irritation, business
Abstract

Summary: A novel alcohol-based antiseptic and a novel water-based antiseptic lotion, both with a synergistic combination of antimicrobial ingredients containing 0.2% benzethonium chloride, were evaluated using the standard time-kill method against 25 FDA-specified challenge microorganisms. The purpose of the testing was to determine whether a non-alcohol product could have equivalent rapid and broad-spectrum kill to a traditional alcohol sanitizer.Both the alcohol- and water-based products showed rapid and broad-spectrum antimicrobial activity. The average 15-s kill was 99.999% of the challenge organism for the alcohol-based antiseptic and 99.971% for the water-based antiseptic. The alcohol-based product demonstrated 100% of peak efficacy (60 s) within the first 15 s, whereas the water-based product showed 99.97%. The novel alcohol-based antiseptic reduced concentrations of 100% of organisms by 99.999%, whereas the water-based antiseptic lotion showed the same reduction for 96% of organisms.A novel water-based antiseptic product demonstrated equivalent rapid, broad-spectrum antimicrobial activity to an alcohol-based sanitizer and provided additional benefits of reduced irritation, persistent effect, and greater efficacy against common viruses. The combination of rapid, broad-spectrum immediate kill and persistent efficacy against pathogens may have significant clinical benefit in limiting the spread of disease. Keywords: Benzethonium chloride, Broad-spectrum antimicrobial activity, Persistence, Hand sanitizer

Published
2014
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3. A Marine Sulfate-Reducing Bacterium Producing Multiple Antibiotics: Biological and Chemical Investigation

Author

Yi Zhang, Chenyan Zhao, Xiaoliang Wang, Zeping Xie, Xiaojie Gu, and Jun Mu

Subjects
Nystatin, food.ingredient, medicine.drug_class, Microorganism, Antibiotics, Phthalic Acids, Pharmaceutical Science, Microbial Sensitivity Tests, Article, Microbiology, food, Drug Discovery, broad-spectrum antimicrobial activity, medicine, Agar, Bioassay, marine sulfate-reducing bacterium, Agar diffusion test, Food science, Desulfovibrio desulfuricans, lcsh:QH301-705.5, Pharmacology, Toxicology and Pharmaceutics (miscellaneous), biology, Sulfur-Reducing Bacteria, mono-n-butyl phthalate, biology.organism_classification, Antimicrobial, Anti-Bacterial Agents, lcsh:Biology (General), Fermentation, identification, Ampicillin, isolation, Bacteria, Thymine
Abstract

A marine sulfate-reducing bacterium SRB-22 was isolated by means of the agar shake dilution method and identified as Desulfovibrio desulfuricans by morphological, physiological and biochemical characteristics and 16S rDNA analysis. In the bioassay, its extract showed broad-spectrum antimicrobial activity using the paper disc agar diffusion method. This isolate showed a different antimicrobial profile than either ampicillin or nystatin and was found to produce at least eight antimicrobial components by bioautography. Suitable fermentation conditions for production of the active constituents were determined to be 28 day cultivation at 25 degrees C to 30 degrees C with a 10% inoculation ratio. Under these conditions, the SRB-22 was fermented, extracted and chemically investigated. So far an antimicrobial compound, mono-n-butyl phthalate, and an inactive compound, thymine, have been isolated and characterized.

Published
2009

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