1. Combinatorial Peptide Microarray Synthesis Based on Microfluidic Impact Printing
- Author
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Li, Jiannan, Zhao, Siwei, Yang, Gaomai, Liu, Ruiwu, Xiao, Wenwu, Disano, Paolo, Lam, Kit S, and Pan, Tingrui
- Subjects
Organic Chemistry ,Chemical Sciences ,Biotechnology ,Amines ,Combinatorial Chemistry Techniques ,Humans ,Integrin alpha4beta1 ,Jurkat Cells ,Microfluidic Analytical Techniques ,Peptide Library ,Peptides ,Printing ,Three-Dimensional ,Protein Array Analysis ,Surface Properties ,peptide synthesis ,microfluidic ,peptide microarrays ,printing ,cancer targeting ,integrin binding ,Biochemistry and cell biology ,Medicinal and biomolecular chemistry ,Organic chemistry - Abstract
In this Research Article, a novel inkjet printing technique, micro impact printing (MI printing), is applied for the first time to combinatorial peptide microarray synthesis on amine functionalized microdisc arrays through standard Fmoc chemistry. MI printing shows great advantages in combinatorial peptide microarray synthesis compared with other printing techniques, including (1) a disposable cartridge; (2) a small spot size (80 μm) increases array density; (3) minimal loading volume (0.6 μL) and dead volume (
- Published
- 2019