Search

Your search keyword '"Nanoaprticles"' showing total 2 results
Start Over Descriptor "Nanoaprticles" Topic microglia
2 results on '"Nanoaprticles"'

1. Early modulation of pro-inflammatory microglia by minocycline loaded nanoparticles confers long lasting protection after spinal cord injury.

Author

Papa S, Caron I, Erba E, Panini N, De Paola M, Mariani A, Colombo C, Ferrari R, Pozzer D, Zanier ER, Pischiutta F, Lucchetti J, Bassi A, Valentini G, Simonutti G, Rossi F, Moscatelli D, Forloni G, and Veglianese P

Subjects
Animals, Behavior, Animal drug effects, Cell Movement drug effects, Chemokine CCL2 metabolism, Disease Models, Animal, Disease Progression, Macrophages drug effects, Mice, Inbred C57BL, Microglia drug effects, Models, Biological, Nerve Regeneration drug effects, Phenotype, Polyesters chemistry, Spinal Cord Injuries pathology, Spinal Cord Injuries physiopathology, Inflammation pathology, Microglia pathology, Minocycline therapeutic use, Nanoparticles chemistry, Spinal Cord Injuries drug therapy
Abstract

Many efforts have been performed in order to understand the role of recruited macrophages in the progression of spinal cord injury (SCI). Different studies revealed a pleiotropic effect played by these cells associated to distinct phenotypes (M1 and M2), showing a predictable spatial and temporal distribution in the injured site after SCI. Differently, the role of activated microglia in injury progression has been poorly investigated, mainly because of the challenges to target and selectively modulate them in situ. A delivery nanovector tool (poly-ε-caprolactone-based nanoparticles) able to selectively treat/target microglia has been developed and used here to clarify the temporal and spatial involvement of the pro-inflammatory response associated to microglial cells in SCI. We show that a treatment with nanoparticles loaded with minocycline, the latter a well-known anti-inflammatory drug, when administered acutely in a SCI mouse model is able to efficiently modulate the resident microglial cells reducing the pro-inflammatory response, maintaining a pro-regenerative milieu and ameliorating the behavioral outcome up to 63 days post injury. Furthermore, by using this selective delivery tool we demonstrate a mechanistic link between early microglia activation and M1 macrophages recruitment to the injured site via CCL2 chemokine, revealing a detrimental contribution of pro-inflammatory macrophages to injury progression after SCI., (Copyright © 2015 Elsevier Ltd. All rights reserved.)

Published
2016
Full Text
View/download PDF

2. Early modulation of pro-inflammatory microglia by minocycline loaded nanoparticles confers long lasting protection after spinal cord injury

Author

Davide Moscatelli, Giulio Alfredo Simonutti, Pietro Veglianese, Francesca Pischiutta, Andrea Bassi, Diego Pozzer, Gianluca Valentini, Gianluigi Forloni, Claudio Colombo, Filippo Rossi, Simonetta Papa, Elisa R. Zanier, Jacopo Lucchetti, Nicolò Panini, Alessandro Mariani, Massimiliano De Paola, Ilaria Caron, Eugenio Erba, and Raffaele Ferrari

Subjects
0301 basic medicine, Chemokine, Polyesters, Nanoaprticles, Inflammation, Macrophages, Microglia, Minocycline, Spinal cord injury, Biomaterials, Bioengineering, Ceramics and Composites, Mechanics of Materials, Biophysics, CCL2, Models, Biological, 03 medical and health sciences, 0302 clinical medicine, Cell Movement, medicine, Animals, Distribution (pharmacology), Chemokine CCL2, Spinal Cord Injuries, Behavior, Animal, biology, business.industry, medicine.disease, Phenotype, Nerve Regeneration, Mice, Inbred C57BL, Disease Models, Animal, 030104 developmental biology, medicine.anatomical_structure, Immunology, Disease Progression, biology.protein, Nanoparticles, medicine.symptom, business, Neuroscience, 030217 neurology & neurosurgery, medicine.drug
Abstract

Many efforts have been performed in order to understand the role of recruited macrophages in the progression of spinal cord injury (SCI). Different studies revealed a pleiotropic effect played by these cells associated to distinct phenotypes (M1 and M2), showing a predictable spatial and temporal distribution in the injured site after SCI. Differently, the role of activated microglia in injury progression has been poorly investigated, mainly because of the challenges to target and selectively modulate them in situ. A delivery nanovector tool (poly-ε-caprolactone-based nanoparticles) able to selectively treat/target microglia has been developed and used here to clarify the temporal and spatial involvement of the pro-inflammatory response associated to microglial cells in SCI. We show that a treatment with nanoparticles loaded with minocycline, the latter a well-known anti-inflammatory drug, when administered acutely in a SCI mouse model is able to efficiently modulate the resident microglial cells reducing the pro-inflammatory response, maintaining a pro-regenerative milieu and ameliorating the behavioral outcome up to 63 days post injury. Furthermore, by using this selective delivery tool we demonstrate a mechanistic link between early microglia activation and M1 macrophages recruitment to the injured site via CCL2 chemokine, revealing a detrimental contribution of pro-inflammatory macrophages to injury progression after SCI.

Published
2016

Catalog

Books, media, physical & digital resources
See catalog results