1. MicroRNA-155-5p is associated with oral squamous cell carcinoma metastasis and poor prognosis.
- Author
-
Baba O, Hasegawa S, Nagai H, Uchida F, Yamatoji M, Kanno NI, Yamagata K, Sakai S, Yanagawa T, and Bukawa H
- Subjects
- Antigens, CD, Biomarkers, Tumor genetics, Cadherins biosynthesis, Cadherins genetics, Carcinoma, Squamous Cell metabolism, Carcinoma, Squamous Cell pathology, Cell Line, Tumor, Cell Movement genetics, Cell Proliferation genetics, Epithelial-Mesenchymal Transition, Female, Head and Neck Neoplasms metabolism, Head and Neck Neoplasms pathology, Humans, Lymph Nodes, Lymphatic Metastasis, Male, MicroRNAs antagonists & inhibitors, MicroRNAs biosynthesis, MicroRNAs genetics, Middle Aged, Mouth Neoplasms metabolism, Mouth Neoplasms pathology, Neoplasm Metastasis, Prognosis, STAT3 Transcription Factor biosynthesis, STAT3 Transcription Factor genetics, Squamous Cell Carcinoma of Head and Neck, Suppressor of Cytokine Signaling 1 Protein biosynthesis, Suppressor of Cytokine Signaling 1 Protein genetics, Transfection, Carcinoma, Squamous Cell genetics, Head and Neck Neoplasms genetics, MicroRNAs metabolism, Mouth Neoplasms genetics
- Abstract
Background: Abnormal miRNA expression was recently implicated in the metastasis of oral squamous cell carcinoma (OSCC) and with a poor prognosis. The initiation of the invasion-metastasis cascade involves epithelial-mesenchymal transition (EMT). Our aim was to clarify how miRNA, especially miR-155-5p misexpression contributes to OSCC metastasis through EMT., Methods: We collected tumor samples from 73 subjects with OSCC. The samples were analyzed by quantitative reverse-transcription polymerase chain reaction (qRT-PCR), and correlations between miR-155-5p levels and clinical characteristics were investigated. OSCC cell lines were analyzed by miRNA microarray and by transfection with a miR-155-5p mimic or inhibitor, followed by proliferation and wound-healing migration assays. qRT-PCR analyses of EMT makers in cells transfected with miR-155-5p inhibitor were performed., Results: We found high miR-155-5p expression in tissue samples from subjects with OSCC that had metastasized to cervical lymph nodes. HSC-3 cells also strongly expressed miR-155-5p. The epithelial marker E-cadherin was strongly expressed in HSC-3 cells transfected with miR-155-5p inhibitor, and we observed elevated SOCS1 and decreased STAT3 expression in these cells., Conclusions: Our results suggest that miR-155-5p causes OSCC to metastasize, and could serve as a novel therapeutic target for OSCC., (© 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2016
- Full Text
- View/download PDF