1. BMSCs-derived exosomes carrying miR-668-3p promote progression of osteoblasts in osteonecrosis of the femoral head: Expression of proteins CD63 and CD9.
- Author
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Qiu Y, Luo Y, Guo G, Meng J, Bao N, and Jiang H
- Subjects
- Animals, Rats, Osteogenesis genetics, Cell Differentiation genetics, Male, Femur Head metabolism, Femur Head pathology, Gene Expression Regulation, MicroRNAs genetics, MicroRNAs metabolism, Exosomes metabolism, Exosomes genetics, Mesenchymal Stem Cells metabolism, Tetraspanin 29 metabolism, Tetraspanin 29 genetics, Tetraspanin 30 metabolism, Tetraspanin 30 genetics, Osteoblasts metabolism, Cell Proliferation, Femur Head Necrosis metabolism, Femur Head Necrosis genetics, Femur Head Necrosis pathology
- Abstract
Recently, exosomes that are derived from bone marrow mesenchymal stem cells (BMSCs) have garnered considerable interest due to their significant roles in the processes of bone regeneration and repair. Among the various molecular components present within these exosomes, miR-668-3p has emerged as a pivotal microRNA that may be instrumental in modulating the function and proliferation of osteoblasts, the cells responsible for bone formation. The primary objective of this research was to examine the enhancing effects of BMSC-derived exosomes that are enriched with miR-668-3p on the advancement of osteoblasts in the context of osteonecrosis of the femoral head. Furthermore, the study aimed to analyze how the expression of specific exosomal proteins, namely CD63 and CD9, influences this biological process. To conduct the investigation, BMSCs were isolated from healthy rat models, followed by the extraction of their secreted exosomes. The subsequent phase of the study involved assessing the proliferation and differentiation of osteoblasts by introducing the exosomes enriched with miR-668-3p into an experimental setup representing osteonecrosis of the femoral head. The findings revealed that exosomes derived from BMSCs, which contained miR-668-3p, significantly enhanced the proliferation of osteoblasts as well as the expression of key osteogenic marker genes. Notably, the levels of CD63 and CD9 proteins were markedly increased in the treated groups, indicating that the mechanisms underlying this promotion might involve cell adhesion and the endocytic uptake of exosomes., Competing Interests: Declaration of competing interest The authors declare no competing interests., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
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