1. Human amniotic mesenchymal stem cells inhibit immune rejection injury from allogeneic mouse heart transplantation: A preliminary study on the microRNA expression.
- Author
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Wang H, Mao X, Zhong Y, Zhao X, Li C, Jiang J, Hong Z, Wang N, and Wang F
- Subjects
- Animals, Mice, Humans, Mice, Inbred BALB C, Cells, Cultured, Disease Models, Animal, Mice, Inbred C57BL, Graft Survival immunology, Female, Male, Heart Transplantation, MicroRNAs genetics, MicroRNAs metabolism, Graft Rejection immunology, Mesenchymal Stem Cells metabolism, Mesenchymal Stem Cells immunology, Mesenchymal Stem Cell Transplantation, Transplantation, Homologous, Amnion cytology
- Abstract
Background: Mesenchymal stem cell therapy is a new treatment for immune rejection in heart transplantation. The aim of this paper is to investigate the effect of human amniotic mesenchymal stem cells (hAMSCs) on alleviating immune rejection of allogeneic heart transplantation in mice and its possible underlying mechanism., Methods: We injected hAMSCs into cervical ectopic heart transplantation model mice via tail vein to observe the survival time, the pathological changes of donor myocardium, and the fluorescent distribution of hAMSCs after the transplantation. MicroRNAs (miRs) with significantly differential expression were obtained by RNA sequencing and bioinformatic analysis, and a dual luciferase reporter gene assay together with real-time quantitative PCR (qRT-PCR) was performed to verify the relationship between miRs and their targeting genes., Results: The intervention of hAMSCs prolonged the graft survival time and alleviated the pathological damage of the donor heart. The injected hAMSCs were distributed mainly in the liver, spleen, and kidney, only a very small portion in the donor and recipient hearts. In the allogeneic transplantation models, the expression of miR-34b-5p significantly increased after hAMSC treatment. MiR-34b-5p showed a knockdown effect on gene Fc gamma receptor 2B (FCGR2B)., Conclusions: hAMSCs can reduce the immune rejection injury after allogeneic heart transplantation. This effect may be associated with the upregulation of miR-34b-5p expression to knock down its targeting gene FCGR2B., Competing Interests: Declaration of competing interest The authors declare that they have no competing interests., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
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