1. Ultrasensitive miRNA detection: A novel DNA nanomachine using split-type molecular beacons-mediated cascade amplification for cancer diagnostics.
- Author
-
Huang L, Xu W, Huang X, Yang B, Yu H, Xu H, and Huang L
- Subjects
- Humans, DNA chemistry, DNA genetics, Limit of Detection, Biomarkers, Tumor blood, Biomarkers, Tumor genetics, MicroRNAs analysis, MicroRNAs blood, Nucleic Acid Amplification Techniques, Neoplasms diagnosis, Neoplasms genetics
- Abstract
MicroRNAs (miRNAs) are crucial regulators in various pathological and physiological processes, and their misregulation is a hallmark of many diseases. In this study, we introduce an advanced DNA nanomachine using split-type molecular beacons (STMBs) for sensitive detection of miR-21, a key biomarker in cancer diagnostics. Utilizing an innovative STMB-mediated cascade strand displacement amplification (STMB-CSDA) technique, our approach offers a powerful means for the precise quantification of miRNAs, using miR-21 as a primary example. The system operates through target-induced linkage of STMBs, initiating a series of strand displacement amplifications resulting in exponential signal amplification. Coupled with the precision of T4 DNA ligase, this mechanism translates minimal miRNA presence into significant fluorescence signals, offering detection sensitivity as low as 5.96 pM and a dynamic range spanning five orders of magnitude. Characterized by its high specificity, which includes the ability to identify single-base mismatches, along with its user-friendly design, our method represents a significant leap forward in miRNA analysis and molecular diagnostics. Its successful application in examining total RNA from cancer cells and clinical serum samples demonstrates its immense potential as a groundbreaking tool for early cancer detection and gene expression studies, paving the way for the next generation of non-invasive diagnostics in personalized healthcare., Competing Interests: Declaration of competing interest No conflict of interest exits in the submission of this manuscript, and manuscript is approved by all authors for publication. We declare that the work described was original research that has not been published previously, and not under consideration for publication elsewhere, in whole or in part. We agree to pay page charges and color publication fees if our paper is accepted., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
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