1. MiR-425-5p suppression of Crebzf regulates oocyte aging via chromatin modification.
- Author
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Jueraitetibaike K, Tang T, Ma R, Zhao S, Wu R, Yang Y, Huang X, Cheng X, Zhou C, Zhang H, Zheng L, Ge X, Chen L, and Yao B
- Subjects
- Female, Animals, Mice, Aging genetics, Aging physiology, Chromatin metabolism, Chromatin genetics, Cellular Senescence physiology, Cellular Senescence genetics, Infertility, Female genetics, Oxidative Stress, Histones metabolism, MicroRNAs genetics, MicroRNAs metabolism, Oocytes metabolism
- Abstract
Female infertility due to declining oocyte quality with age remains a significant challenge for patients and physicians, despite extensive research efforts. Recent studies suggest that microRNAs (miRNAs), which respond to various stressors in the aging process, may provide a promising solution. With the approval of small RNA drugs for clinical use, miRNA-based treatment of oocyte aging appears to be a viable option. Through high-throughput sequencing, miR-425-5p was identified as the only miRNA elevated under natural aging and oxidative stress. Microinjection of inhibitors to inhibit miR-425-5p effectively improved compromised phenotypes of old oocytes in vitro. Further investigation revealed that Crebzf acts as a mediator of miR-425-5p's age-related functions in old oocytes. In vivo treatment with miR-425-5p antagomirs significantly improved impaired oocyte development in reproductively old females by targeting Crebzf. Single-cell RNA sequencing revealed that Crebzf plays a vital role in regulating mRNAs targeting histone H3, trimethylated lysine 4 (H3K4me3), a crucial marker for transcriptional silencing. Overexpression of miR-425-5p could hinder oocyte maturation by downregulating Crebzf expression and disrupting transcriptional regulation. Our findings provide new insights into the potential of miR-425-5p antagomirs as a treatment for female infertility and highlight an elegant mechanism by which miR-425-5p inhibition of Crebzf inhibits a developmental switch in GV oocytes by regulating a group of histone methyltransferase mRNAs., (© 2023. The Author(s).)
- Published
- 2024
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