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Whether aging or Parkinson's disease (PD) affects the responses of peripheral blood mononuclear cells (PBMCs) to immunosuppression by bone marrow-derived mesenchymal stem cell (BM-MSCs) and which cytokines are more effective in inducing BM-MSCs to be immunosuppressive remains to be elucidated. PBMCs were isolated from healthy young (age 26–35), healthy middle-aged (age 56–60) and middle-aged PD-affected individuals. All the recruits were male. The mitogen-stimulated PBMCs and proinflammatory cytokine-pretreated BM-MSCs were co-cultured. The PBMC proliferation was measured using Cell Counting Kit-8, while the cytokine secretion was assayed by cytometric bead array technology. The immunosuppressive ability of BM-MSCs was confirmed in young healthy, middle-aged healthy and middle-aged PD-affected individuals. Among the three groups, the PBMC proliferation and cytokine secretion of the young healthy group were suppressed more significantly compared with those of the middle-aged healthy and middle-aged PD-affected group. No significant differences were identified in the PBMC proliferation and cytokine secretion between the patients with PD and the middle-aged healthy subjects. Interferon (IFN)-γ synergized with tumor necrosis factor (TNF)-α, interleukin (IL)-1α or IL-1β was more effective than either one alone, and the combinations of IFN-γ + IL-1α and IFN-γ + IL-1β were more effective than IFN-γ + TNF-α in inducing BM-MSCs to inhibit PBMC proliferation. The results of the present study suggested that aging, rather than PD, affects the response of PBMCs toward the suppression of BM-MSC, at least in middle-aged males. Patients with PD aged 56–60 remain eligible for anti-inflammatory BM-MSC-based therapy. Treatment of BM-MSCs with IFN-γ + IL-1α or IFN-γ + IL-1β prior to transplantation may result in improved immunosuppressive effects.

To realize the effectiveness and security of Tobramycin and Dexamethasone Eye Ointment for blepharitis treatment. Design Case control studies. Participants 148 patients be diagnosed as blepharitis including 81 cases as research group and 67 cases as control group.Multi-center randomized controlled clinical trial. The patients met the inclusion criteria from First Hospital Peking University, Beijing Tongren Hospital, Southwest Hospital, Ophthalmology Center Zhongshan University and Third Hospital Peking University were divided into two groups in Aug. to Dec. 2011. Tobramycin dexamethasone eye ointment was applied to eye lid in research group while patients in control group used tobramycin eye ointment. All patients were informed to apply warm compress to the lids, mechanically washing to the eyelids and artificial tears. At the beginning, the 7(th) day, the 14(th) day and the 28(th) day of treatment, observation of symptom, sign and side effect were recorded.Before treatment the difference of the symptom scores of burning sensation, tears, photophobia and itch of two groups has no statistically significant (t = 1.87, 0.43, -0.64, 0.93, P0.05), but the symptom scores have a statistically significant decrease (t = 1.99 - 6.90, P0.05) at the 7(th) day, 14(th) day, 28(th) day. Before treatment the difference of signs scores have no statistically significant except the term of new blood vessels (t = 2.32, P = 0.02) while all the signs scores have a statistically significant decrease at the 7(th) day, 14(th) day, 28(th) day. Side effect: the number of patients with elevated intraocular pressure of research and control group is 3 and 1 respectively and the ratio is 3.7% and 1.5%.The tobramycin dexamethasone eye ointment can improve signs and symptoms of blepharitis patients. Some patients have to face with the risk of elevated intraocular pressure and it's necessary to monitor the intraocular pressure.

To analyze the clinical characterization of ocular cicatricial pemphigoid (OCP).It was a retrospective series case study. Five consecutive patients referred for the evaluation of possible OCP from January 2005 to October 2008 in Departments of Ophthalmology and Dermatology of Peking University First Hospital. History and clinical characterization of 5 cases (10 eyes) OCP having been misdiagnosed were analyzed to find the causes of misdiagnosis.All of cases were diagnosed as chronic conjunctivitis during the early stages of the diseases, one case was diagnosed as Stevens-Johnson syndrome and one as Sjögren syndrome during the later stage. It was two to five years from the first time to see a doctor to definite diagnosis. All of cases have been prescribed antibiotic eye drops for a long times, one case has been undergone three times trichiasis operation and made the disease progression. Among the five patients with OCP, 3 eyes were diagnosed Stage II, 5 eyes Stage III, 2 eyes Stage IV. Three cases were positive of bacterial culture. Only in 1 case, there was slight increase of iron protein as tumor mark. Inflammation was controlled by the end of the study, but cicatrization of 2 cases still progressed.Manifestation of OCP can mimic chronic conjunctivitis during the early stages, it is important to pay high attention to OCP, misdiagnosis may be stopped.

To investigate the etiology, clinical features, treatment and prognosis of toxic anterior segment syndrome (TASS).It was a retrospective series case study. The clinical data of eight definite diagnosed TASS cases were retrospectively analyzed.Among eight TASS cases, seven were post cataract surgery cases and one was post cornea penetrating injury. Three cases were caused by residual povidone iodine on instruments, 2 cases resulted from the misuse of distilled water as intraocular irrigating liquid during cataract surgery, 2 cases were produced by the countercurrent of antibiotic solution via the cornea-scleral incision into anterior chamber during subconjunctival injection at the end of the surgery, and 1 case was induced by the injection of the distilled water into the anterior chamber at the end of the surgery. Three TASS cases occurred during operation and 5 cases occurred at 1 day after operation. All eight cases suffered from the painless blurred vision. Three cases occurred during operation presented with decrease of corneal transparency and depigmentation of iris. On the first day after operation, all cases had diffuse corneal stroma edema and severe anterior uveitis. Dexamethasone 0.1% or prednisolone acetate 1% eye drops, three times per day or one time per hour was used in all cases. Carteolol 2% eye drop, two times per day, was used for the cases with ocular hypertension. The cornea was clear in 6 cases, but corneal endothelial decompensation in 2 cases after therapy.Various toxic agents injected into anterior chamber by misuse can result in TASS. All these misuse can be avoided. Early diagnosis and proper management may be important to improve the prognosis of TASS.

To investigate the prevalence of lid-wiper epitheliopathy (LWE) and risk factors in dry eye patients.One hundred forty-one patients (141 eyes) were recruited in Peking University First Hospital from September 2006 to March 2007. These patients were divided into three groups based on the dry eye symptoms and the results of break up time, Schirmer I test and corneal fluorescein staining. Group A: 60 patients (60 eyes) with no dry eye symptoms and normal results of BUT, Schirmer I test and corneal fluorescein staining. Group B: 51 patients (51 eyes) with dry eye symptoms and normal results of BUT, Schirmer I test and corneal fluorescein staining. Group C: 30 patients (30 eyes) with dry eye. The patients in group A and group B were further divided into two subgroups based on wearing contact lens. Subgroup A1:30 patients (30 eyes) with no contact lens wearers; Subgroup A2:30 patients (30 eyes) with contact lens wearers. Subgroup B1:21 patients (21 eyes) with no contact lens wearers; Subgroup B2: 30 patients (30 eyes) with contact lens wearers. Lid-wiper staining was graded in all patients following the instillation of fluorescein and lissamine green dyes. Impression cytology (IC) was made up in the patients in group C and the patients with LWE in group B.The prevalence of LWE was 18.3% (11 patients), 86.3% (44 patients) and 100.0% (30 patients) in group A, B, C respectively. The prevalence of LWE was 13.3% (4 patients), 23.3% (7 patients), 81.0% (17 patients) and 90.0% (27 cases) in subgroup A, A2, B1, and B2. There was a significant statistical difference in the prevalence of LWE among groups A, B and C (X2 = 78.256, P0.01). There were no statistical difference in the prevalence of LWE between subgroup A2 and subgroup A1 (X2 = 1.002, P = 0.253), subgroup B2 and subgroup B1 (X2 = 0.854, P = 0.301). In group B, IC was graded as mark zero in 74.0% of patients with LWE, as mark one in 26.0% of patients with LWE. In group C, IC was graded as mark one in 40.0% of patients, as mark two in 50.0% of patients, and as mark three in 10.0% of patients. There was a significant statistical difference in grade of IC between group B and C (M-W = 36.0, P0.01). The result of the corneal fluorescein staining was positive in 18.8% of patients with LWE. The result of lissamine green staining was positive in 14. 1% of patients with LWE. However, the result of both fluorescein and lissamine green staining was 67. 1% in patients with LWE.The prevalence of LWE is higher in patients with dry eye symptoms and normal results of BUT, Schirmer I test and corneal fluorescein staining. LWE may be an early manifestation of the dry eye.

To investigate corneal limbus changes in long-term soft contact lens wearers using laser confocal microscope.All examinees were chosen from people who received pre-refractive surgery examination or regular physical examination in Department of Ophthalmology of Peking University First Hospital during October to November, 2004 and May to October, 2006. The contact lens wearer (CLW) group consisted of 22 eyes of 22 patients (age distribution, 23 - 50 years) with a history of refractive errors and more than ten-year soft contact lens wear. Thirty-eight eyes of 38 patients (age distribution, 18 - 52 years) with refractive errors but no history of contact lens wear were observed as the controls. After detailed examinations of slit lamp biomicroscopy, superior and inferior limbus were examined by laser confocal microscope. Corneal microstructure and cell morphology were analyzed, and cell densities were measured.Images of corneal limbus could be observed clearly in both groups. The arrangement of limbal epithelium in CLW group showed irregular as compared to the control group. Epithelial microcystic changes and highly reflective dots among epithelium were found in CLW group which were not found in the control group. The Langerhans cell density of both superior limbus and inferior limbus in CLW group were higher than that of the control group (P0.05). There were highly reflective microdot deposits in the limbal corneal stroma in CLW group which were not appeared in the control group. Keratocyte density in anterior stroma of both superior and inferior limbus in CLW group was lower than that of the control group (P0.05). Keratocyte density in posterior stroma of both superior and inferior limbus in CLW group was lower than that of the control group (P0.05). The average stroma thickness of both superior and inferior limbus in CLW group was also lower than that of the control group (P0.05).Long-term soft contact lens wear can induce many changes in corneal limbus, such as microcystic changes in epithelial cells, microdot deposits in corneal stroma, increase of Langerhans cell density and decrease of keratocyte density, etc.

To investigate the effect of lens opacity on the performance of the multi-focal visual evoked potential (mfVEP) test in patients with glaucoma and its relationship with the Humphrey's automated static perimetry.This is a prospective, non-randomized study. Twenty-two patients were enrolled with moderate cataract and primary glaucoma scheduled for cataract surgery with or without glaucoma surgery. Humphrey visual field and mfVEP test were preformed before surgery and after 6 - 8 weeks of post-operation. Lens opacity was evaluated with slit lamp according to LOCS III (lens opacity classification system, version III) by the same ophthalmologist.The improvement of AccuMap objective visual field was noted after lens removal in glaucoma patients. The amplitude of the AccuMap was significantly (P0.01) increased after cataract surgery compared to pre-operation (382.6 nV +/- 146.7 nV vs 308.0 nV +/- 96.6 nV). AccuMap severity index (ASI) was significantly (P0.001) decreased following lens removal compared with pre-operation (48.6 +/- 42.4 vs 90.0 +/- 54.8, P0.001). Latency was little changed before and after surgery (1207.5 ms +/- 67.7 ms, 1191.2 ms +/- 61.9 ms, respectively, P = 0.289). There were no correlations between the changes of amplitude and the degree of lens opacity (Spearman correction, P = 0.09). For Humphrey visual field, the mean deviation (MD) of pre- and post-operative was significantly (P0.001) affected by lens opacity (-12.2 +/- 7.8, -9.6 +/- 8.8, respectively, P0.001), but pattern standard deviation (PSD) had no such changes (6.5 +/- 3.2, 6.3 +/- 4.0, respectively, P = 0.748). There were well correlated between the ASI and the MD both pre- or post-operation (Pearson correction, P = 0.013, P0.001), and between the ASI changes and the MD changes after lens removal (Pearson correction, P = 0.017).Lens opacity affects the AccuMap objective visual field in patients with glaucoma, especially the amplitude of the AccuMap. The ASI is sensitively correlated with the MD of Humphrey perimetry.

This study was designed to evaluate the long-term effect of phacoemulsification, lens implantation combined with trabeculectomy augmented with 5-Fluorouracil (phaco-trab-5-Fu) in Asian glaucoma patients with primary open-angle glaucoma (POAG) and primary angle-closure glaucoma (PACG).This retrospective study included 71 consecutive patients who underwent phaco-trab-5-Fu in Singapore National Eye Center between January 1996 and December 1998. Patients with follow-up time less than 6 months were excluded from the study.The mean follow-up time was (53.1 +/- 20.9) months. Postoperative IOP was significantly lower than preoperation [(15.0 +/- 4.0) mmHg (1 mmHg = 0.133 kPa), (20.3 +/- 5.4) mmHg, respectively, P0.01]. Postoperative number of antiglaucoma medications was also significantly decreased than preoperation (P0.01). IOP of 1 to 5 years postoperation dropped significantly from that of preoperation (P0.01). Complete success (CS) rate of phaco-trab-5-Fu was 84.2%, 62.7%, 55.3% for 1, 3, 5 years, respectively. There were neither significant difference found in PACG group and POAG group, or in single-incision and separate-incision of CS rate. But mean IOP of last follow-up was significantly different between PACG and POAG [mean IOP (13.8 +/- 4.6) mmHg, (16.2 +/- 3.7) mmHg, respectively, P = 0.02]. According to LogMAR chart, mean preoperative visual acuity (VA) and postoperative VA were 0.75 +/- 0.40 and 0.46 +/- 0.46, respectively. Postoperative VA was significantly improved (P0.01). In visual field, MD were (-16.6 +/- 8.8) dB and (-17.6 +/- 10.1) dB at preoperation and postoperation, respectively. PSD were (6.2 +/- 2.9) dB and (6.5 +/- 3.2) dB correspondingly. There were no significant difference in both MD and PSD (P = 0.55, 0.64, respectively). One patient had early endophthalmitis and required vitrectomy. Two late-onset of hypotony were due to choroidal effusion and wound leak.Phacoemulsification combined with trabeculectomy and 5-Fu could be a successful and safe treatment for patients with cataract and primary glaucoma in a long run. There is no difference in success rate in PACG and POAG, or in single incision or separate incision in such combined surgery.