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Summary: Steinernema tielingense was found to be widely distributed in Japan. The molecular characteristics of the nematodes isolated from Japan are basically in accordance with those mentioned in the original description of S. tielingense. However, the Japanese isolates are morphologically distinct from the type population by the following characters: length of the infective juvenile, 711 (671-769) μ m, excretory pore of adult males and infective juveniles located posterior mid-pharynx, D% < 50%, smaller H value of 35% and shorter spicules in adult males of both generations. Although discrepancies in morphology and morphometric data between the Chinese isolate and Japanese isolate were observed, the molecular data matched and on this basis the Japanese isolate was identified as being conspecific with S. tielingense. The symbiotic bacterium isolated from the Japanese population of S. tielingense was identified as Xenorhabdus bovienii. [ABSTRACT FROM AUTHOR]

Keywords: Iotonchium cateniforme; Iotonchium laccariae; Iotonchium russulae; molecular; phylogeny; Tylenchomorpha EN Iotonchium cateniforme Iotonchium laccariae Iotonchium russulae molecular phylogeny Tylenchomorpha 1205 1209 5 11/23/21 20211001 NES 211001 I Iotonchium i Cobb, 1920 currently contains 11 species, four of which have been reported in Japan: I I. cateniforme i Tsuda & Futai, 1999, I I. laccariae i Tsuda & Futai, 2005, I I. russulae i Tsuda & Futai, 2005, and I I. ungulatum i Aihara, 2001. The DNA material of I I. cateniforme i , I I. laccariae i , and I I. russulae i was prepared as described previously (Iwahori I et al i ., 2000), and near-full-length of 18S ribosomal RNA (small subunit: SSU) was determined for these three species according to the methods in Iwahori I et al i . According to this classification, I I. ungulatum i and I I. russula i e belong to I Fungiotonchium i , and I I. cateniforme i belongs to I Iotonchium sensu stricto i (Tsuda & Futai, 1999, 2000, 2005; Aihara, 2001). In particular, I I. ungulatum i forms characteristic nematode galls on mushrooms of carnivorous fungi, I Pleurotus i spp. (Tsuda I et al i ., 1996; Tsuda & Futai, 2000). [Extracted from the article]

Summary: Contortylenchus genitalicola is a unique species within the genus. It parasitises the ovary or testis of the longhorn beetle, Monochamus alternatus , and has a neotylenchid-like culturable mycetophagous phase within its life cycle, while the other species in the genus parasitise bark beetles and have a typical unculturable allantonematid life cycle. Because the species was described based solely on morphology, it was necessary to confirm the phylogenetic and taxonomic status of C. genitalicola. A molecular phylogenetic analysis of the species based on ribosomal RNA sequences revealed that C. genitalicola forms a well-supported clade with Contortylenchus spp. isolated from bark beetles, an unidentified allantonematid from Iran, and ' Deladenus cf. laricis ' isolated from the metallic wood-boring beetle, Agrilus macer LeConte (Coleoptera: Buprestidae), in the USA. The generic status of C. genitalicola was supported by phylogenetic analysis, and the analysis also suggests that the diversity of the genus could be higher than currently expected. [ABSTRACT FROM AUTHOR]

Summary: We isolated two tylenchid species belonging to the Sychnotylenchinae, Sychnotylenchus hibisci n. sp. and Neoditylenchus xiphydriae n. sp., from the bark beetle Ernoporus corpulentus and wood wasp Xiphydria ogasawarai , respectively. Sychnotylenchus hibisci n. sp. is characterised by its moderately stout body in both male and female, gubernaculum to spicule length ratio of one-third or less, crustaformeria structure composed of four columns with six large, rounded cells, and a broad female tail with a rounded terminus. The new species is closely related to S. ulmi , with which it shares body shape and gubernaculum to spicule length ratio, but is distinguished by the female tail shape and several morphometric values. Neoditylenchus xiphydriae n. sp. is characterised by its oval to leaf-shaped male bursa, male tail tip reaching the distal end of, and protruding beyond the bursa, a long post-vulval uterine sac (PUS), and a conical female tail with a narrowly rounded terminus. The species is typologically similar to N. pinophilus but is distinguished by its male spicule shape, gubernaculum to spicule length ratio, and longer PUS. Phylogenetically, the Sychnotylenchinae belongs under the Anguinidae (superfamily Sphaerularioidea) and is closely related to several Ditylenchus , Neotylenchus , Neomisticius , and Ficotylus species. [ABSTRACT FROM AUTHOR]