1. Quantitative proteomics study of protective effects of grape seed procyanidin B2 on diabetic cardiomyopathy in db/db mice
- Author
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Rui-jie Qin, Baoying Li, Wen-bin Yin, Mei Cheng, Fei Yu, Haiqing Gao, Xiao-li Li, Sisi Luan, and Qian Cai
- Subjects
Male ,Proteomics ,medicine.medical_specialty ,Diabetic Cardiomyopathies ,Cardiac fibrosis ,Quantitative proteomics ,Cardiomyopathy ,Apoptosis ,Protective Agents ,medicine.disease_cause ,Applied Microbiology and Biotechnology ,Biochemistry ,Antioxidants ,Catechin ,Analytical Chemistry ,Mice ,chemistry.chemical_compound ,Internal medicine ,Diabetic cardiomyopathy ,Diabetes mellitus ,medicine ,Animals ,Biflavonoids ,Proanthocyanidins ,Molecular Biology ,Procyanidin B2 ,Grape Seed Extract ,business.industry ,Organic Chemistry ,Type 2 Diabetes Mellitus ,General Medicine ,medicine.disease ,Oxidative Stress ,Endocrinology ,chemistry ,Evaluation Studies as Topic ,business ,Oxidative stress ,Biotechnology - Abstract
Diabetic cardiomyopathy is one of the major complications of diabetes mellitus. Oxidative stress appears to play a substantial role in cardiomyopathy. Grape seed procyanidin B2 (GSPB2) has been known as an anti-oxidant in treating diabetes mellitus; however, little is known about its effects and underlying mechanisms on diabetic cardiomyopathy. The present study is to explore the molecular targets of GSPB2 responsible for the anti-oxidative effects in db/db mice by quantitative proteomics. GSPB2 (30 mg/kg body weight/day) were intragastric administrated to db/db mice for 10 weeks. Proteomics of the heart tissue extracts by isobaric tags for relative and absolute quantification analysis was obtained from db/db mice. Our study provides important evidence that GSPB2 protect against cardiomyopathy in diabetes mellitus, which are believed to result from regulating the expression of key proteins involving cardiac fibrosis and proliferation. GSPB2 could be expected to become novel clinical application in fighting against diabetic cardiomyopathy.
- Published
- 2014