1. Granulocyte colony-stimulating factor directly affects human monocytes and modulates cytokine secretion
- Author
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Tomoko Taguchi, Koichi Ishimoto, Nobutaka Kiyokawa, Kyoko Suzuki, Hiroo Fujita, Toyo Suzuki, Junichiro Fujimoto, Junya Fujimura, Yuichiro Yamashiro, Takaomi Sekino, Kenichi Mimori, Yohko U. Katagiri, Masahiro Saito, and Hideki Nakajima
- Subjects
Lipopolysaccharides ,Cancer Research ,Chemokine ,medicine.medical_specialty ,medicine.medical_treatment ,Lipopolysaccharide Receptors ,Monocytes ,Cell Line ,Proinflammatory cytokine ,Internal medicine ,Granulocyte Colony-Stimulating Factor ,Genetics ,medicine ,Humans ,Secretion ,RNA, Messenger ,Molecular Biology ,DNA Primers ,biology ,Reverse Transcriptase Polymerase Chain Reaction ,Monocyte ,Interleukin ,Cell Biology ,Hematology ,Recombinant Proteins ,Kinetics ,medicine.anatomical_structure ,Endocrinology ,Cytokine ,Receptors, Granulocyte Colony-Stimulating Factor ,Immunology ,biology.protein ,Cytokines ,Interleukin 19 ,Cytokine secretion ,Chemokines - Abstract
Objective Recent reports have indicated that monocytes express receptors for the granulocyte colony-stimulating factor (G-CSF). The direct effects of G-CSF on cytokine secretion in monocytes were examined. Materials and Methods A monocytic cell line NOMO-1 that secretes multiple cytokines upon stimulation with lipopolysaccharide (LPS) was used. Normal human monocytes were purified by negative selection using magnetic beads. Cells pretreated with or without G-CSF were stimulated with LPS, and the subsequent concentrations of cytokines and chemokines in supernatants were determined by sandwich enzyme-linked immunosorbent assay. Results NOMO-1 cells were found to express receptors for G-CSF. Although G-CSF stimulation did not induce cytokine secretion, pretreatment with G-CSF significantly attenuated LPS-stimulated secretion of the proinflammatory cytokines tumor necrosis factor-α and interleukin (IL)-12 in NOMO-1 cells. Simultaneously, however, G-CSF pretreatment apparently enhanced LPS-induced secretion of IL-10 and monocyte chemoattractant protein-1, whereas secretions of IL-1β, IL-6, and IL-8 were unaffected. When normal human monocytes from healthy volunteers were similarly examined, marked individual variations in LPS-induced secretion of cytokines were observed. Although some exceptions exist, a similar tendency as to the effects of G-CSF treatment on cytokine secretions as that in NOMO-1 cells was observed in human monocytes. Conclusions Our data suggest that G-CSF directly affects monocytes and modulates their cytokine secretion. NOMO-1 cells can provide an alternate model for in vitro culture of monocytes to investigate the effects of G-CSF on cytokine secretion by these cells.
- Published
- 2002
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