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8 results on '"Carmen Gómez-González"'

1. Identification of a HIV-1 circulating BF1 recombinant form (CRF75_BF1) of Brazilian origin that also circulates in Southwestern Europe

Author

Joan Bacqué, Elena Delgado, Horacio Gil, Sofía Ibarra, Sonia Benito, Isabel García-Arata, María Moreno-Lorenzo, Ester Sáez de Adana, Carmen Gómez-González, Mónica Sánchez, Vanessa Montero, and Michael M. Thomson

Subjects
HIV-1, circulating recombinant forms, molecular epidemiology, phylogeny, phylodynamics, recombination, Microbiology, QR1-502
Abstract

IntroductionThe high recombinogenic potential of HIV-1 has resulted in the generation of countless unique recombinant forms (URFs) and around 120 reported circulating recombinant forms (CRFs). Here we identify through analyses of near full-length genomes (NFLG) a new HIV-1 CRF derived from subtypes B and F1.MethodsHIV-1 protease-reverse transcriptase (Pr-RT) sequences were obtained by RT-PCR amplification from plasma RNA. Near full-length genome sequences were obtained after amplification by RT-PCR in 5 overlapping fragments. Phylogenetic sequence analyses were performed via maximum likelihood. Mosaic structures were analyzed by bootscanning and phylogenetic analyses of genome segments. Temporal and geographical estimations of clade emergence were performed with a Bayesian coalescent method.ResultsThrough phylogenetic analyses of HIV-1 Pr-RT sequences obtained by us from samples collected in Spain and downloaded from databases, we identified a BF1 recombinant cluster segregating from previously reported CRFs comprising 52 viruses, most from Brazil (n = 26), Spain (n = 11), and Italy (n = 9). The analyses of NFLG genomes of 4 viruses of the cluster, 2 from Spain and 2 from Italy, allowed to identify a new CRF, designated CRF75_BF1, which exhibits a complex mosaic structure with 20 breakpoints. All 4 patients harboring CRF75_BF1 viruses studied by us had CD4+ T-cell lymphocyte counts below 220/mm3 less than one year after diagnosis, a proportion significantly higher (p = 0.0074) than the 29% found in other patients studied in Spain by us during the same period. The origin of the clade comprising CRF75_BF1 and related viruses was estimated around 1984 in Brazil, with subsequent introduction of CRF75_BF1 in Italy around 1992, and migration from Italy to Spain around 1999.ConclusionA new HIV-1 CRF, designated CRF75_BF1, has been identified. CRF75_BF1 is the 6th CRF of South American origin initially identified in Western Europe, reflecting the increasing relationship of South American and European HIV-1 epidemics. The finding of low CD4+ T-cell lymphocyte counts early after diagnosis in patients harboring CRF75_BF1 viruses warrants further investigation on the virulence of this variant.

Published
2023
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2. Diverse Large HIV-1 Non-subtype B Clusters Are Spreading Among Men Who Have Sex With Men in Spain

Author

Elena Delgado, Sonia Benito, Vanessa Montero, María Teresa Cuevas, Aurora Fernández-García, Mónica Sánchez-Martínez, Elena García-Bodas, Francisco Díez-Fuertes, Horacio Gil, Javier Cañada, Cristina Carrera, Jesús Martínez-López, Marcos Sintes, Lucía Pérez-Álvarez, Michael M. Thomson, The Spanish Group for the Study of New HIV Diagnoses, Josefa Muñoz, María Carmen Nieto, María Zuriñe Zubero, Silvia Hernáez-Crespo, Luis Elorduy Otazua, Leyre López Soria, Koldo Agirrebengoa, María José López de Goicoechea, José Mayo, Gustavo Cilla, Julio Arrizabalaga, José Antonio Iribarren, María Jesús Bustinduy, María Julia Echevarría, María Jesús Lezaun, José Joaquín Portu, Carmen Gómez-González, Ana Mariño, Patricia Ordóñez, Hortensia Álvarez, Nieves Valcarce, Ángeles Cañizares, María Ángeles Castro, María Amparo Coira, José López-Álvarez, Ramón Rabunal, Juan García-Costa, Ricardo Fernández-Rodríguez, Raúl Rodríguez-Pérez, Jorge Guitián, Antonio Ocampo, Celia Miralles, Sonia Pérez-Castro, Matilde Trigo, Julio Diz-Arén, María Ángeles Pallarés, Carmen Ezpeleta Baquedano, Aitziber Aguinaga, María Gracia Ruiz de Alda, Jorge del Romero, Carmen Rodríguez, Mar Vera, Óscar Ayerdi, María Isabel García-Arata, Santiago Prieto-Menchero, Esther Culebras, Iciar Rodríguez-Avial, Raquel Téllez, Miguel Górgolas, Manuel Fernández-Guerrero, Olalla Calabia, Rosa García-Delgado, Sara María Quevedo, Lucía Puente, Manuel Álamo, Alfonso Alfange, Sara de la Fuente, Carmen Hinojosa, Carlos Dueñas, Begoña Monteagudo, Edita Sánchez, Carmen Ramos Sánchez, Pablo Bachiller, Helmuth Guillén, Teresa Martínez-Domínguez, Rosa Martínez-González, José Ramón Blanco, Miriam Blasco, Ana María Martínez-Sapiña, Diego Ortega Larrea, César Gómez-Hernando, José Largo-Pau, Fernando Buñuel, and Ana Infante

Subjects
HIV-1, molecular epidemiology, phylogeny, phylodynamics, men who have sex with men, subtypes, Microbiology, QR1-502
Abstract

In Western Europe, the HIV-1 epidemic among men who have sex with men (MSM) is dominated by subtype B. However, recently, other genetic forms have been reported to circulate in this population, as evidenced by their grouping in clusters predominantly comprising European individuals. Here we describe four large HIV-1 non-subtype B clusters spreading among MSM in Spain. Samples were collected in 9 regions. A pol fragment was amplified from plasma RNA or blood-extracted DNA. Phylogenetic analyses were performed via maximum likelihood, including database sequences of the same genetic forms as the identified clusters. Times and locations of the most recent common ancestors (MRCA) of clusters were estimated with a Bayesian method. Five large non-subtype B clusters associated with MSM were identified. The largest one, of F1 subtype, was reported previously. The other four were of CRF02_AG (CRF02_1; n = 115) and subtypes A1 (A1_1; n = 66), F1 (F1_3; n = 36), and C (C_7; n = 17). Most individuals belonging to them had been diagnosed of HIV-1 infection in the last 10 years. Each cluster comprised viruses from 3 to 8 Spanish regions and also comprised or was related to viruses from other countries: CRF02_1 comprised a Japanese subcluster and viruses from 8 other countries from Western Europe, Asia, and South America; A1_1 comprised viruses from Portugal, United Kingom, and United States, and was related to the A1 strain circulating in Greece, Albania and Cyprus; F1_3 was related to viruses from Romania; and C_7 comprised viruses from Portugal and was related to a virus from Mozambique. A subcluster within CRF02_1 was associated with heterosexual transmission. Near full-length genomes of each cluster were of uniform genetic form. Times of MRCAs of CRF02_1, A1_1, F1_3, and C_7 were estimated around 1986, 1989, 2013, and 1983, respectively. MRCA locations for CRF02_1 and A1_1 were uncertain (however initial expansions in Spain in Madrid and Vigo, respectively, were estimated) and were most probable in Bilbao, Spain, for F1_3 and Portugal for C_7. These results show that the HIV-1 epidemic among MSM in Spain is becoming increasingly diverse through the expansion of diverse non-subtype B clusters, comprising or related to viruses circulating in other countries.

Published
2019
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3. Molecular Epidemiology of Staphylococcus aureus Bacteremia: Association of Molecular Factors With the Source of Infection

Author

Dafne Pérez-Montarelo, Esther Viedma, Nieves Larrosa, Carmen Gómez-González, Enrique Ruiz de Gopegui, Irene Muñoz-Gallego, Rafael San Juan, Nuria Fernández-Hidalgo, Benito Almirante, and Fernando Chaves

Subjects
Staphylococcus aureus bacteremia, bacteremia source, molecular epidemiology, clonal complex, virulence factors, Microbiology, QR1-502
Abstract

Staphylococcus aureus bacteremia (SAB) is associated with high morbidity and mortality, which varies depending on the source of infection. Nevertheless, the global molecular epidemiology of SAB and its possible association with specific virulence factors remains unclear. Using DNA microarrays, a total of 833 S. aureus strains (785 SAB and 48 colonizing strains) collected in Spain over a period of 15 years (2002–2017) were characterized to determine clonal complex (CC), agr type and repertoire of resistance and virulence genes in order to provide an epidemiological overview of CCs causing bloodstream infection, and to analyze possible associations between virulence genes and the most common sources of bacteremia. The results were also analyzed by acquisition (healthcare-associated [HA] and community-acquired [CA]), methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) strains, and patient age (adults vs. children). Our results revealed high clonal diversity among SAB strains with up to 28 different CCs. The most prevalent CCs were CC5 (30.8%), CC30 (20.3%), CC45 (8.3%), CC8 (8.4%), CC15 (7.5%), and CC22 (5.9%), which together accounted for 80% of all cases. A higher proportion of CC5 was found among HA strains than CA strains (35.6 vs. 20.2%, p < 0.001). CC5 was associated with methicillin resistance (14.7 vs. 79.4%, p < 0.001), whereas CC30, CC45, and CC15 were correlated with MSSA strains (p < 0.001). Pathogen-related molecular markers significantly associated with a specific source of bacteremia included the presence of sea, undisrupted hlb and isaB genes with catheter-related bacteremia; sed, splE, and fib genes with endocarditis; undisrupted hlb with skin and soft tissue infections; and finally, CC5, msrA resistance gene and hla gene with osteoarticular source. Our study suggests an association between S. aureus genotype and place of acquisition, methicillin resistance and sources of bloodstream infection, and provides a valuable starting point for further research insights into intrinsic pathogenic mechanisms involved in the development of SAB.

Published
2018
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4. Identification of CRF89_BF, a new member of an HIV-1 circulating BF intersubtype recombinant form family widely spread in South America

Author

Gema Cenzual, Elena Bereciartua, Vanessa Montero, Luis Elorduy, Ana Martínez-Sapiña, Silvia Hernáez, Michael M. Thomson, Carmen Rodríguez, Carmen Gómez-González, Aurora Fernández-García, Iciar Rodríguez-Avial, Muñoz J, María Luisa Giménez-Alarcón, Esther Culebras, Elena Delgado, Marcos Pérez-Losada, Horacio Gil, María Maiques-Camarero, Lucía Pérez-Álvarez, Ismael Fernández-Miranda, Miren Z. Zubero-Sulibarria, Sonia Benito, María Moreno-Lorenzo, Jorge del Romero, Carmen Martín-Salas, Mónica Sánchez, Elena García-Bodas, José J. García-Irure, Instituto de Salud Carlos III, Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF), Basque Government (España), and Red de Investigación Cooperativa en Investigación en Sida (España)

Subjects
0301 basic medicine, Science, 030106 microbiology, Human immunodeficiency virus (HIV), Human-Immunodeficiency-Virus, Biology, medicine.disease_cause, Type-1, Article, law.invention, 03 medical and health sciences, law, parasitic diseases, medicine, Clade, Viral evolution, South American origin, Genetics, Molecular Epidemiology, Diversity, Multidisciplinary, Retrovirus, Phylogenetic tree, Phylogenetic Analysis, Men, Subtype B, 030104 developmental biology, Buenos Aires, Recombinant DNA, Medicine, Sex, Population-Dynamics
Abstract

Author Correction: Identifcation of CRF89_BF, a new member of an HIV‑1 circulating BF intersubtype recombinant form family widely spread in South America. Sci Rep. 2021 Aug 3;11(1):16115. DOI: 10.1038/s41598-021-95679-z. Circulating recombinant forms (CRFs) contribute substantially to the HIV-1 pandemic. Among 105 CRFs described in the literature, 16 are BF intersubtype recombinants, most of South American origin, of which CRF12_BF is the most widely spread. A BF recombinant cluster identified in Bolivia was suggested to represent a new CRF_BF. Here we find that it belongs to a larger cluster incorporating 39 viruses collected in 7 countries from 3 continents, 22 of them in Spain, most from Bolivian or Peruvian individuals, and 12 in South America (Bolivia, Argentina, and Peru). This BF cluster comprises three major subclusters, two associated with Bolivian and one with Peruvian individuals. Near full-length genome sequence analyses of nine viruses, collected in Spain, Bolivia, and Peru, revealed coincident BF mosaic structures, with 13 breakpoints, 6 and 7 of which coincided with CRF12_BF and CRF17_BF, respectively. In a phylogenetic tree, they grouped in a clade closely related to these CRFs, and more distantly to CRF38_BF and CRF44_BF, all circulating in South America. These results allowed to identify a new HIV-1 CRF, designated CRF89_BF. Through phylodynamic analyses, CRF89_BF emergence was estimated in Bolivia around 1986. CRF89_BF is the fifth CRF member of the HIV-1 recombinant family related to CRF12_BF. This work was funded through Acción Estratégica en Salud Intramural (AESI), Instituto de Salud Carlos III, projects “Estudios sobre vigilancia epidemiológica molecular del VIH-1 en España”, PI16CIII/00033, and “Epidemiología molecular del VIH en España y su utilidad para investigaciones biológicas y en vacunas”, PI19CIII/00042; Red de Investigación en SIDA (RIS), Instituto de Salud Carlos III, Subdirección General de Evaluación y Fondo Europeo de Desarrollo Regional (FEDER), Plan Nacional I+D+I, project RD16ISCIII/0002/0004; and scientific agreement with Osakidetza-Servicio Vasco de Salud, Government of Basque Country, MVI 1001/16. Sí

Published
2021

5. Identification of a New HIV-1 BC Intersubtype Circulating Recombinant Form (CRF108_BC) in Spain

Author

Ana Martínez-Sapiña, Ester Sáez de Adana, Sofía Ibarra, Gustavo Cilla, Joseba Portu-Zapirain, Sonia Benito, Mónica Sánchez, Mireia De la Peña, Michael M. Thomson, Horacio Gil, José Antonio Iribarren, Elena García-Bodas, Javier E. Cañada, Carmen Gómez-González, Elena Delgado, Andrés Canut-Blasco, Instituto de Salud Carlos III, Red Española de Investigación en SIDA, European Regional Development Fund (ERDF/FEDER), Gobierno Vasco, Comunidad de Madrid, Red de Investigación Cooperativa en Investigación en Sida (España), Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF), Basque Government (España), and Comunidad de Madrid (España)

Subjects
0301 basic medicine, Most recent common ancestor, Genotype, Human immunodeficiency virus (HIV), lcsh:QR1-502, HIV Infections, Genome, Viral, Biology, medicine.disease_cause, Genome, lcsh:Microbiology, Article, law.invention, Coalescent theory, circulating recombinant form, 03 medical and health sciences, 0302 clinical medicine, law, Virology, HIV-1 phylogeny, medicine, Humans, Public Health Surveillance, 030212 general & internal medicine, Genetic variability, Viremia, Phylogeny, HIV-1 genetic diversity, Genetics, Recombination, Genetic, Molecular Epidemiology, Phylogenetic tree, HIV-1 molecular epidemiology, Genetic Variation, Sequence Analysis, DNA, Phylogeography, 030104 developmental biology, Infectious Diseases, Spain, Recombinant DNA, HIV-1, Identification (biology)
Abstract

The extraordinary genetic variability of human immunodeficiency virus type 1 (HIV-1) group M has led to the identification of 10 subtypes, 102 circulating recombinant forms (CRFs) and numerous unique recombinant forms. Among CRFs, 11 derived from subtypes B and C have been identified in China, Brazil, and Italy. Here we identify a new HIV-1 CRF_BC in Northern Spain. Originally, a phylogenetic cluster of 15 viruses of subtype C in protease-reverse transcriptase was identified in an HIV-1 molecular surveillance study in Spain, most of them from individuals from the Basque Country and heterosexually transmitted. Analyses of near full-length genome sequences from six viruses from three cities revealed that they were BC recombinant with coincident mosaic structures different from known CRFs. This allowed the definition of a new HIV-1 CRF designated CRF108_BC, whose genome is predominantly of subtype C, with four short subtype B fragments. Phylogenetic analyses with database sequences supported a Brazilian ancestry of the parental subtype C strain. Coalescent Bayesian analyses estimated the most recent common ancestor of CRF108_BC in the city of Vitoria, Basque Country, around 2000. CRF108_BC is the first CRF_BC identified in Spain and the second in Europe, after CRF60_BC, both phylogenetically related to Brazilian subtype C strains. This work was funded through Acción Estratégica en Salud Intramural (AESI), Instituto de Salud Carlos III, projects “Estudios sobre vigilancia epidemiológica molecular del VIH- 1 en España,” PI16CIII/00033, and “Epidemiología molecular del VIH-1 en España y su utilidad para investigaciones biológicas y en vacunas”, PI19CIII/00042; Red de Investigación en SIDA (RIS), Instituto de Salud Carlos III, Subdirección General de Evaluación y Fondo Europeo de Desarrollo Regional (FEDER), Plan Nacional I + D + I, project RD16ISCIII/0002/0004; and scientific agreement with Osakidetza-Servicio Vasco de Salud, Government of Basque Country, MVI 1001/16. JC was supported by the Social European Fund through the Youth Employment Operational Program and the Youth Employment Initiative and by the Comunidad de Madrid. Sí

Published
2021

6. Molecular Epidemiology of Staphylococcus aureus Bacteremia: Association of Molecular Factors With the Source of Infection

Author

Irene Muñoz-Gallego, Fernando Chaves, Rafael San Juan, Nuria Fernández-Hidalgo, Enrique Ruiz de Gopegui, Esther Viedma, Benito Almirante, Nieves Larrosa, Carmen Gómez-González, and Dafne Pérez-Montarelo

Subjects
0301 basic medicine, Microbiology (medical), medicine.medical_specialty, 030106 microbiology, lcsh:QR1-502, virulence factors, Virulence, bacteremia source, Human leukocyte antigen, Biology, Microbiology, molecular epidemiology, lcsh:Microbiology, 03 medical and health sciences, Genotype, Epidemiology, medicine, Endocarditis, Gene, Original Research, Molecular epidemiology, clonal complex, medicine.disease, Bacteremia, Staphylococcus aureus bacteremia
Abstract

Staphylococcus aureus bacteremia (SAB) is associated with high morbidity and mortality, which varies depending on the source of infection. Nevertheless, the global molecular epidemiology of SAB and its possible association with specific virulence factors remains unclear. Using DNA microarrays, a total of 833 S. aureus strains (785 SAB and 48 colonizing strains) collected in Spain over a period of 15 years (2002-2017) were characterized to determine clonal complex (CC), agr type and repertoire of resistance and virulence genes in order to provide an epidemiological overview of CCs causing bloodstream infection, and to analyze possible associations between virulence genes and the most common sources of bacteremia. The results were also analyzed by acquisition (healthcare-associated [HA] and community-acquired [CA]), methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) strains, and patient age (adults vs. children). Our results revealed high clonal diversity among SAB strains with up to 28 different CCs. The most prevalent CCs were CC5 (30.8%), CC30 (20.3%), CC45 (8.3%), CC8 (8.4%), CC15 (7.5%), and CC22 (5.9%), which together accounted for 80% of all cases. A higher proportion of CC5 was found among HA strains than CA strains (35.6 vs. 20.2%, p < 0.001). CC5 was associated with methicillin resistance (14.7 vs. 79.4%, p < 0.001), whereas CC30, CC45, and CC15 were correlated with MSSA strains (p < 0.001). Pathogen-related molecular markers significantly associated with a specific source of bacteremia included the presence of sea, undisrupted hlb and isaB genes with catheter-related bacteremia; sed, splE, and fib genes with endocarditis; undisrupted hlb with skin and soft tissue infections; and finally, CC5, msrA resistance gene and hla gene with osteoarticular source. Our study suggests an association between S. aureus genotype and place of acquisition, methicillin resistance and sources of bloodstream infection, and provides a valuable starting point for further research insights into intrinsic pathogenic mechanisms involved in the development of SAB., This work was supported by the Health Research Fund, Department of Health, Spain; Agency for Health Technology Assessment and Research (PI15/02013 and PI15/02125) and Instituto de Salud Carlos III, Subdireccion General de Redes y Centros de Investigacion Cooperativa, Ministerio de Economia y Competitividad, Spanish Network for Research in Infectious Diseases (REIPI RD16/0016-0002; 0003; 0004) and cofunded by the European Regional Development Fund (FEDER).

Published
2018

7. Molecular epidemiology of Staphylococcus aureus bacteremia in children, Spain: low risk of methicillin resistance

Author

Marta Barrios, Laura Barrado, Carmen Gómez-González, Pablo Rojo, Mª Angeles Orellana, Fernando Chaves, and Patricia Brañas

Subjects
Microbiology (medical), Male, Staphylococcus aureus, Molecular epidemiology, business.industry, Staphylococcus aureus bacteremia, Staphylococcal Infections, Methicillin resistance, Microbiology, Diabetes Complications, Infectious Diseases, Medicine, Humans, Female, business
Published
2013

8. Long Persistence of Methicillin-Susceptible Strains of Staphylococcus aureus Causing Sepsis in a Neonatal Intensive Care Unit▿

Author

Francisca Sanz, Concepción Alba, Carmen Gómez-González, Joaquín R. Otero, and Fernando Chaves

Subjects
Microbiology (medical), Male, Staphylococcus aureus, Neonatal intensive care unit, Meticillin, Epidemiology, education, Staphylococcal infections, medicine.disease_cause, Microbiology, Sepsis, Intensive Care Units, Neonatal, medicine, Humans, Phylogeny, Antibacterial agent, Cross Infection, Molecular epidemiology, business.industry, Infant, Newborn, Staphylococcal Infections, medicine.disease, Bacteremia, Female, Methicillin Resistance, business, medicine.drug
Abstract

Molecular epidemiology of Staphylococcus aureus strains causing bacteremia in neonates during 2002 to 2005 revealed seven clones, with four MSSA clones responsible for 80% of the cases. Some clones persisted or reappeared throughout the study. Three bacteremic clones were found colonizing health care workers (HCWs), particularly clone C, which was harbored by at least 15% of HCWs.

Published
2007

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