1. Typing Method for the QUB11a Locus of Mycobacterium tuberculosis: IS6110 Insertions and Tandem Repeat Analysis.
- Author
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Maeda-Mitani, Eriko, Murakami, Koichi, Oishi, Akira, Etoh, Yoshiki, Sera, Nobuyuki, and Fujimoto, Shuji
- Subjects
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MYCOBACTERIAL disease diagnosis , *TUBERCULOSIS diagnosis , *LOCUS of control , *MOLECULAR biology , *MOLECULAR epidemiology , *RESEARCH funding , *TUBERCULOSIS - Abstract
QUB11a is used as a locus for variable number of tandem repeats (VNTR) analysis of Mycobacterium tuberculosis Beijing lineage. However, amplification of QUB11a occasionally produces large fragments (>1,400 bp) that are not easily measured by capillary electrophoresis because of a lack of the typical stutter peak patterns that are used for counting repeat numbers. IS6110 insertion may complicate VNTR analysis of large QUB11a fragments in M. tuberculosis. We established a method for determining both tandem repeat numbers and IS6110 insertion in the QUB11a locus of M. tuberculosis using capillary electrophoresis analysis and BsmBI digestion. All 29 large QUB11a fragments (>1,200 bp) investigated contained IS6110 insertions and varied in the number of repeats (18 patterns) and location of IS6110 insertions. This method allows VNTR analysis with high discrimination. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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