1. Phylogenetic analysis of partial RNA-polymerase blocks II and III of Rabies virus isolated from the main rabies reservoirs in Brazil.
- Author
-
Carnieli P Jr, de Novaes Oliveira R, de Oliveira Fahl W, de Carvalho Ruthner Batista HB, Scheffer KC, Iamamoto K, and Castilho JG
- Subjects
- Animals, Brazil epidemiology, Canidae virology, Chiroptera virology, Dogs, Lyssavirus classification, Lyssavirus genetics, Molecular Sequence Data, Rabies virology, Rabies virus enzymology, Rabies virus isolation & purification, Sequence Analysis, DNA, Species Specificity, Disease Reservoirs virology, Molecular Epidemiology, Phylogeny, RNA-Dependent RNA Polymerase genetics, Rabies epidemiology, Rabies virus genetics
- Abstract
This study describes the results of the sequencing and analysis of segments of Blocks II and III of the RNA polymerase L gene of Rabies virus isolates from different reservoir species of Brazil. The phylogenetic relations of the virus were determined and a variety of species-specific nucleotides were found in the analyzed areas, but the majority of these mutations were found to be synonymous. However, an analysis of the putative amino acid sequences were shown to have some characteristic mutations between some reservoir species of Brazil, indicating that there was positive selection in the RNA polymerase L gene of Rabies virus. On comparing the putative viral sequences obtained from the Brazilian isolates and other Lyssavirus, it was determined that amino acid mutations occurred in low-restriction areas. This study of the L gene of Rabies virus is the first to be conducted with samples of virus isolates from Brazil, and the results obtained will help in the determination of the phylogenetic relations of the virus.
- Published
- 2012
- Full Text
- View/download PDF