Search

[Display omitted] • Comparative investigation of Paeonia ostii stamens in different regions of China. • Bioactive constitutes were analyzed through integrated metabolomics approach. • Global Natural Products Social (GNPS) platform enhanced metabolite identification. • Eight predominant chemical markers were selected for quality control. • Unveiled the antibacterial, antitumor, and anti-neuroinflammation potentials. Paeonia ostii is a widely cultivated medicinal and oilseed crop in China. The stamens of P. ostii are important by-products of this crop and conventionally used as tea with putative beneficial effects on human health. However, the in-depth understanding of P. ostii stamens is scarce. In this study, comparative analysis was carried out to systematically characterize the bioactive constituents and biological activities of P. ostii stamens from different geographical regions of China. Seventy-seven secondary metabolites were tentatively identified through untargeted metabolomic analysis based on MS/MS molecular similar networking and other approaches. Targeted metabolomics conjugated with principal component analysis revealed that eight bioactive compounds, including oxypaeoniflorin, pentagalloyl glucose (PGG), paeoniflorin, and albiflorin, were the primary chemical markers for quality control. Habitats substantially affected the content of PGG, and geographical factor of Nujiang (Southern China) significantly promoted the accumulation of PGG. The extracts from peony stamens across all geographical regions exhibited potent antioxidant, antibacterial, antitumor, and anti-neuroinflammation properties, which were strongly correlated with bioactive compounds. Our results provide new insights into P. ostii stamens to be used for pharmaceutical purposes. [ABSTRACT FROM AUTHOR]

[Display omitted] • Metabolite identification and quantification through integrated LC-MS strategy. • Metabolites were identified based on MS/MS molecular networking. • Paeoniflorin and resveratrol are specially localized in seed kernel and coat. • Fruit pod sand seed coat showed significantly stronger activities than seed kernel. • The fruit has potential utilization in food and pharmaceutical industries. Tree peony seed, traditionally used for edible oil production, is rich in α-linolenic acid. However, little attention is given to the fruit by-products during seed oil production. The present work aimed to comprehensively investigate the phytochemical constituents and multiple biological activities of different parts of tree peony fruits harvested from Paeonia ostii and Paeonia rockii. 130 metabolites were rapidly identified through UPLC-Triple-TOF-MS on the basis of MS/MS molecular networking. Metabolite quantification was performed through the targeted approach of HPLC-ESI-QQQ-MS. Eight chemical markers were screened via principal component analysis (PCA) for distinguishing species and tissues. Interestingly, two dominant compounds, paeoniflorin and trans -resveratrol, are specially localized in seed kernel and seed coat, respectively. Unexpectedly, the extracts of fruit pod and seed coat showed significantly stronger antioxidant, antibacterial, and anti-neuroinflammatory activities than seed kernel from both P. ostii and P. rockii. Our work demonstrated that tree peony fruit is promising natural source of bioactive components and provided its potential utilization in food and pharmaceutical industries. [ABSTRACT FROM AUTHOR]