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A novel acidic heteropolysaccharide (LCP-90-1) was isolated and purified from a traditional "heat-clearing" Chinese medicine, Lysimachia christinae Hance. LCP-90-1 (Mw , 20.65 kDa) was composed of Man, Rha, GlcA, Glc, Gal, and Ara, with relative molar ratios of 1.00: 3.00: 11.62: 1.31: 1.64: 5.24. The backbone consisted of 1,4- α -D-Glc p A, 1,4- α -D-Glc p , 1,4- β -L-Rha p , and 1,3,5- α -L-Ara f , with three branches of β -D-Gal p -(1 → 4)- β -L-Rha p -(1→, α -L-Ara f -(1→ and α -D-Man p -(1→ attached to the C-5 position of 1,3,5- α -L-Ara f. LCP-90-1 exhibited potent anticomplement activity (CH50 : 135.01 ± 0.68 µg/mL) in vitro , which was significantly enhanced with increased glucuronic acid (GlcA) content in its degradation production (LCP-90-1-A, CH50 : 28.26 ± 0.39 µg/mL). However, both LCP-90-1 and LCP90-1-A were inactivated after reduction or complete acid hydrolysis. These observations indicated the important role of GlcA in LCP-90-1 and associated derivatives with respect to anticomplement activity. Similarly, compared with LCP-90-1, the antioxidant activity of LCP-90-1-A was also enhanced. Thus, polysaccharides with a high content of GlcA might be important and effective substances of L. christinae. [ABSTRACT FROM AUTHOR]

Rhododendron principis leaves have been used as "Dama", a Traditional Tibetan Medicine for treating inflammatory diseases. R. principis crude polysaccharides with anticomplementary activity demonstrated promising anti-inflammatory effects on acute lung injury induced by lipopolysaccharide. R. principis crude polysaccharides significantly decreased the levels of TNF- α and interleukin-6 in both serum and blood and bronchoalveolar lavage fluid in lipopolysaccharide-induced acute lung injury mice by intragastric administration (100 mg/kg). A heteropolysaccharide, ZNDHP, was obtained from R. principis crude polysaccharides with successive anticomplementary activity-guided separation. ZNDHP was characterized as a branched neutral polysaccharide with a backbone composed of → 2)- β -Glc p -(1→, → 2,6)- α -Glc p -(1→, → 6,3)- β -Gal p -(1→, → 2,6)- α -Gal p -(1→, → 6,2)- β -Glc p -(1→, → 4)- α -Glc p -(1→, → 5)- β -Ara f -(1→, → 3,5)- α -Ara f -(1→, and → 4,6)- β -Man p -(1→, and the backbone structure was further confirmed by partial acid hydrolysis. In addition to anticomplementary and antioxidant activities, ZNDHP exhibited potent anti-inflammatory activity by significantly inhibiting the secretion of nitric oxide, TNF- α , interleukin-6, and interleukin-1 β of lipopolysaccharide-treated RAW 264.7 cells. However, all of these activities decreased greatly after partially hydrolyzing, indicating the importance of the multibranched structure for its bioactivity. Therefore, ZNDHP might be an important component of R. principis for treating inflammation. [ABSTRACT FROM AUTHOR]

Three new phenolic glycosides, carvacrol-2- O - β -D-apiofuranosyl-(1 → 6)- β -D-glucopyranoside (1), 1-methyl-3-isopropylphenol-4- O - β -D-apiofuranosyl-(1 → 6)- β -D-glucopyranoside (2), p -methoxythymol-5- O - β -D-apiofuranosyl-(1 → 6)- β -D-glucopyranoside (3), and a pair of new 8- O -4′ neolignan enantiomers (5a / 5b), together with 26 known compounds (4, 6 – 30) were isolated from the roots of Lilium dauricum. The structures of the new compounds were elucidated based on extensive spectroscopic and chemical methods, and the absolute configurations of 5a and 5b were established by electronic circular dichroism analysis. Nine compounds (1, 3, 4, 8, 9, 17, 25, 29, and 30) exhibited potent α -glucosidase inhibitory activity with IC50 values ranging from 73.4 µM to 988.2 µM. Besides, compound 19 displayed strong anticomplementary activity (CH50 : 71.6 µM). [ABSTRACT FROM AUTHOR]