1. Characterization of l-fucose isomerase from Paenibacillus rhizosphaerae to produce l-fuculose from hydrolyzed fucoidan and commercial fucose.
- Author
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Iqbal, Muhammad Waheed, Riaz, Tahreem, Mu, Wanmeng, Mahmood, Shahid, Qi, Xianghui, Ni, Dawei, and Zhang, Wenli
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FUCOSE , *MOLECULAR weights , *PAENIBACILLUS , *ISOMERASES , *UNDARIA pinnatifida , *POLYSACCHARIDES - Abstract
Aims l -Fuculose is a valuable rare sugar that is used to treat a variety of ailments, including HIV, cancer, Hepatitis B, human lysosomal disease (fucosidosis), and cardio-protective medications. The enzymatic approach for the production of l -fuculose using l -fucose as a substrate would be an advantageous method with a wide range of industrial applications. The objective of this study is the characterization of recombinant l -fucose isomerase from Paenibacillus rhizosphaerae (Pa-LFI) for the production of l -fuculose from an inexpensive and natural source (fucoidan) as well as its comparison with commercial l -fucose (Sigma-Aldrich). Methods and results Fucoidan, a fucose-containing polysaccharide (FPs), was isolated from Undaria pinnatifida , subsequently hydrolyzed, and characterized before the enzymatic production of l -fuculose. The results elaborate that FPs contain 35.9% of fucose along with other kinds of monosaccharides. The purified Pa-LFI exhibited a single band at 65 kDa and showed it as a hexamer with a native molecular mass of 396 kDa. The highest activity of 104.5 U mg−1 of Pa-LFI was perceived at a temperature of 50°C and pH 6.5 in the presence of 1 mM of Mn2+. The Pa-LFI revealed a melting temperature (T m) of 75°C and a half-life of 12.6 h at 50°C. It exhibited that Pa-LFI with aldose substrate (l -fucose), has a stronger isomerizing activity, disclosing K m, k cat, and k cat/ K m 86.2 mM, 32 831 min−1, and 335 min−1 mM−1, respectively. After reaching equilibrium, Pa-LFI efficiently catalyzed the reaction to convert l -fucose into l -fuculose and the conversion ratios of l -fuculose from 100 g L−1 of FPs and commercial fucose were around 6% (5.6 g L−1) and 30% (30.2 g L−1), respectively. Conclusions According to the findings of the current study, the Pa-LFI will be useful in the manufacturing of l -fuculose using an effective and easy approach that produces no by-products. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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