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Start Over Author "Woodberry, Megan" Topic monocyte-macrophage precursor cells
2 results on '"Woodberry, Megan"'

1. Deletion of myeloid IRS2 enhances adipose tissue sympathetic nerve function and limits obesity.

Author

Rached MT, Millership SJ, Pedroni SMA, Choudhury AI, Costa ASH, Hardy DG, Glegola JA, Irvine EE, Selman C, Woodberry MC, Yadav VK, Khadayate S, Vidal-Puig A, Virtue S, Frezza C, and Withers DJ

Subjects
Adipose Tissue, Brown physiology, Animals, Catecholamines metabolism, Cells, Cultured, Energy Metabolism, Gene Deletion, Insulin Receptor Substrate Proteins metabolism, Male, Mice, Mice, Inbred C57BL, Signal Transduction, Sympathetic Nervous System physiology, Adipose Tissue, Brown metabolism, Insulin Receptor Substrate Proteins genetics, Monocyte-Macrophage Precursor Cells metabolism, Obesity genetics, Sympathetic Nervous System metabolism
Abstract

Objective: Sympathetic nervous system and immune cell interactions play key roles in the regulation of metabolism. For example, recent convergent studies have shown that macrophages regulate obesity through brown adipose tissue (BAT) activation and beiging of white adipose tissue (WAT) via effects upon local catecholamine availability. However, these studies have raised issues about the underlying mechanisms involved including questions regarding the production of catecholamines by macrophages, the role of macrophage polarization state and the underlying intracellular signaling pathways in macrophages that might mediate these effects., Methods: To address such issues we generated mice lacking Irs2, which mediates the effects of insulin and interleukin 4, specifically in LyzM expressing cells (Irs2 LyzM-/- mice)., Results: These animals displayed obesity resistance and preservation of glucose homeostasis on high fat diet feeding due to increased energy expenditure via enhanced BAT activity and WAT beiging. Macrophages per se did not produce catecholamines but Irs2 LyzM-/- mice displayed increased sympathetic nerve density and catecholamine availability in adipose tissue. Irs2-deficient macrophages displayed an anti-inflammatory transcriptional profile and alterations in genes involved in scavenging catecholamines and supporting increased sympathetic innervation., Conclusions: Our studies identify a critical macrophage signaling pathway involved in the regulation of adipose tissue sympathetic nerve function that, in turn, mediates key neuroimmune effects upon systemic metabolism. The insights gained may open therapeutic opportunities for the treatment of obesity., (Copyright © 2018 The Authors. Published by Elsevier GmbH.. All rights reserved.)

Published
2019
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2. Deletion of myeloid IRS2 enhances adipose tissue sympathetic nerve function and limits obesity

Author

Rached, Marie-Therese, Millership, Steven J, Pedroni, Silvia MA, Choudhury, Agharul I, Costa, Ana SH, Hardy, Darran G, Glegola, Justyna A, Irvine, Elaine E, Selman, Colin, Woodberry, Megan C, Yadav, Vijay K, Khadayate, Sanjay, Vidal-Puig, Antonio, Virtue, Samuel, Frezza, Christian, Withers, Dominic J, Vidal-Puig, Antonio [0000-0003-4220-9577], Frezza, Christian [0000-0002-3293-7397], Apollo - University of Cambridge Repository, Wellcome Trust, and Medical Research Council

Subjects
EXPRESSION, Male, HOMEOSTASIS, lcsh:Internal medicine, Sympathetic Nervous System, Macrophage, Irs2, INSULIN-RECEPTOR SUBSTRATE-2, PROTEIN, AAM, alternatively activated macrophages, Monocyte-Macrophage Precursor Cells, Endocrinology & Metabolism, Mice, Catecholamines, ALTERNATIVELY ACTIVATED MACROPHAGES, HFD, high fat diet, HOMA-IR, homeostatic model assessment-insulin resistance, Adipose Tissue, Brown, Animals, SVF, stromal vascular fraction, BMDM, bone marrow-derived macrophages, Obesity, SAM, sympathetic-associated macrophages, lcsh:RC31-1245, CLAMS, comprehensive lab animal monitoring system, Cells, Cultured, Sympathetic neurons, Inflammation, Science & Technology, ROLES, EE, energy expenditure, BAT, WAT, white adipose tissue, GENE, BETA-CELL, BAT, brown adipose tissue, Mice, Inbred C57BL, ATM, adipose tissue macrophages, Insulin Receptor Substrate Proteins, LPS, lipopolysaccharide, Original Article, CAM, classically activated macrophages, Energy Metabolism, Life Sciences & Biomedicine, SYSTEM, Gene Deletion, Signal Transduction
Abstract

Objective Sympathetic nervous system and immune cell interactions play key roles in the regulation of metabolism. For example, recent convergent studies have shown that macrophages regulate obesity through brown adipose tissue (BAT) activation and beiging of white adipose tissue (WAT) via effects upon local catecholamine availability. However, these studies have raised issues about the underlying mechanisms involved including questions regarding the production of catecholamines by macrophages, the role of macrophage polarization state and the underlying intracellular signaling pathways in macrophages that might mediate these effects. Methods To address such issues we generated mice lacking Irs2, which mediates the effects of insulin and interleukin 4, specifically in LyzM expressing cells (Irs2LyzM−/− mice). Results These animals displayed obesity resistance and preservation of glucose homeostasis on high fat diet feeding due to increased energy expenditure via enhanced BAT activity and WAT beiging. Macrophages per se did not produce catecholamines but Irs2LyzM−/− mice displayed increased sympathetic nerve density and catecholamine availability in adipose tissue. Irs2-deficient macrophages displayed an anti-inflammatory transcriptional profile and alterations in genes involved in scavenging catecholamines and supporting increased sympathetic innervation. Conclusions Our studies identify a critical macrophage signaling pathway involved in the regulation of adipose tissue sympathetic nerve function that, in turn, mediates key neuroimmune effects upon systemic metabolism. The insights gained may open therapeutic opportunities for the treatment of obesity., Highlights • Irs2 deletion in macrophages improves metabolic health and BAT activity in mice. • Irs2-deficient BMDM are protected from M1 polarisation by LPS. • BAT norepinephrine levels and TH density mirror increases in BAT activity. • Cross-talk between macrophages and BAT sympathetic neurons underpin these findings. • Macrophage Irs2 modulates energy homeostasis.

Published
2019

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