1. Differentiation of THP-1 monocytes to macrophages increased mitochondrial DNA copy number but did not increase expression of mitochondrial respiratory proteins or mitochondrial transcription factor A.
- Author
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Okamoto M, Shimogishi M, Nakamura A, Suga Y, Sugawara K, Sato M, Nishi R, Fujisawa A, Yamamoto Y, and Kashiba M
- Subjects
- Cell Differentiation drug effects, Cell Differentiation genetics, Cell Differentiation physiology, DNA Copy Number Variations, DNA-Binding Proteins metabolism, Humans, Macrophages drug effects, Membrane Transport Proteins metabolism, Mitochondria metabolism, Mitochondria ultrastructure, Mitochondrial Precursor Protein Import Complex Proteins, Mitochondrial Proteins metabolism, Monocytes drug effects, RNA, Messenger genetics, RNA, Messenger metabolism, Receptors, Cell Surface metabolism, THP-1 Cells, Tetradecanoylphorbol Acetate pharmacology, Transcription Factors metabolism, Ubiquinone analogs & derivatives, Ubiquinone metabolism, DNA, Mitochondrial genetics, DNA, Mitochondrial metabolism, Macrophages cytology, Macrophages metabolism, Monocytes cytology, Monocytes metabolism
- Abstract
Monocytes are differentiated into macrophages. In this study, mitochondrial DNA copy number (mtDNAcn) levels and downstream events such as the expression of respiratory chain mRNAs were investigated during the phorbol 12-myristate 13-acetate (PMA)-induced differentiation of monocytes. Although PMA treatment increased mtDNAcn, the expression levels of mRNAs encoded in mtDNA were decreased. The levels of mitochondrial transcription factor A mRNA and protein were also decreased. The levels of coenzyme Q10 remained unchanged. These results imply that, although mtDNAcn is considered as a health marker, the levels of mtDNAcn may not always be consistent with the parameters of mitochondrial functions., (Copyright © 2021. Published by Elsevier Inc.)
- Published
- 2021
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