1. Evolutionary history of carbon monoxide dehydrogenase/acetyl-CoA synthase, one of the oldest enzymatic complexes
- Author
-
Panagiotis S. Adam, Simonetta Gribaldo, Guillaume Borrel, Biologie Evolutive de la Cellule Microbienne - Evolutionary Biology of the Microbial Cell, Institut Pasteur [Paris] (IP), Université Paris Diderot - Paris 7 (UPD7), P.S.A. is supported by a PhD fellowship from Paris Diderot University and by funds from the PhD Programme 'Frontières du Vivant (FdV)–Programme Bettencourt.' G.B. is a recipient of a Roux-Cantarini fellowship from the Institut Pasteur. S.G. acknowledges funding from the French National Agency for Research Grant ArchEvol (ANR-16-CE02-0005-01)., We thank Alexander Probst for feedback on an earlier version of the paper, and two anonymous reviewers whose comments helped improve clarity and correctness of the manuscript., ANR-16-CE02-0005,Arch-Evol,Approches phylogenomiques pour étudier l'origine et évolution des Archées(2016), and Institut Pasteur [Paris]
- Subjects
0301 basic medicine ,Enzyme complex ,MESH: Genome, Archaeal ,LUCA ,[SDV]Life Sciences [q-bio] ,MESH: Genome, Bacterial ,Genome ,Corrections ,chemistry.chemical_compound ,Genome, Archaeal ,MESH: Multienzyme Complexes/genetics ,methanogens ,Phylogeny ,MESH: Phylogeny ,Genetics ,Carbon Monoxide ,Multidisciplinary ,Last universal ancestor ,Carbon fixation ,Acetyl-CoA ,MESH: Carbon Cycle ,Aldehyde Oxidoreductases ,Biological Evolution ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,PNAS Plus ,MESH: Bacteria/enzymology ,Wood–Ljungdahl pathway ,Wood-Ljungdahl pathway ,MESH: Aldehyde Oxidoreductases/metabolism ,Carbon monoxide dehydrogenase ,030106 microbiology ,MESH: Bacteria/genetics ,Acetate-CoA Ligase ,Bacterial genome size ,Biology ,MESH: Biological Evolution ,MESH: Aldehyde Oxidoreductases/genetics ,Carbon Cycle ,03 medical and health sciences ,MESH: Carbon Monoxide/metabolism ,Multienzyme Complexes ,MESH: Archaea/genetics ,evolution ,MESH: Acetate-CoA Ligase/metabolism ,MESH: Multienzyme Complexes/metabolism ,MESH: Archaea/enzymology ,Bacteria ,MESH: Acetate-CoA Ligase/genetics ,Archaea ,acetogens ,030104 developmental biology ,chemistry ,biology.protein ,Genome, Bacterial - Abstract
Erratum in : Correction for Adam et al., Evolutionary history of carbon monoxide dehydrogenase/acetyl-CoA synthase, one of the oldest enzymatic complexes. [Proc Natl Acad Sci U S A. 2018]; International audience; Carbon monoxide dehydrogenase/acetyl-CoA synthase (CODH/ ACS) is a five-subunit enzyme complex responsible for the carbonyl branch of the Wood-Ljungdahl (WL) pathway, considered one of the most ancient metabolisms for anaerobic carbon fixation, but its origin and evolutionary history have been unclear. While traditionally associated with methanogens and acetogens, the presence of CODH/ACS homologs has been reported in a large number of un-cultured anaerobic lineages. Here, we have carried out an exhaustive phylogenomic study of CODH/ACS in over 6,400 archaeal and bacterial genomes. The identification of complete and likely functional CODH/ACS complexes in these genomes significantly expands its distribution in microbial lineages. The CODH/ACS complex displays astounding conservation and vertical inheritance over geological times. Rare intradomain and interdomain transfer events might tie into important functional transitions, including the acquisition of CODH/ACS in some archaeal methanogens not known to fix carbon, the tinkering of the complex in a clade of model bacterial acetogens, or emergence of archaeal-bacterial hybrid complexes. Once these transfers were clearly identified, our results allowed us to infer the presence of a CODH/ACS complex with at least four subunits in the last universal common ancestor (LUCA). Different scenarios on the possible role of ancestral CODH/ACS are discussed. Despite common assumptions, all are equally compatible with an autotrophic, mixotrophic, or heterotrophic LUCA. Functional characterization of CODH/ACS from a larger spectrum of bacterial and archaeal lineages and detailed evolutionary analysis of the WL methyl branch will help resolve this issue.
- Published
- 2018