1. Cerebrospinal fluid mtDNA concentrations are increased in multiple sclerosis and were normalized after intervention with autologous hematopoietic stem cell transplantation.
- Author
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Pavlovic I, Zjukovskaja C, Nazir FH, Müller M, Wiberg A, and Burman J
- Subjects
- Humans, DNA, Mitochondrial cerebrospinal fluid, DNA, Mitochondrial therapeutic use, Transplantation, Autologous methods, Mitochondria, Multiple Sclerosis drug therapy, Hematopoietic Stem Cell Transplantation methods
- Abstract
Background: Mitochondrial DNA (mtDNA) is a pro-inflammatory damage-associated molecular pattern molecule and could be an early indicator for inflammation and disease activity in MS. Autologous hematopoietic stem cell transplantation (aHSCT) is a potent treatment for MS, but its impact on mtDNA levels in cerebrospinal fluid (CSF) remains unexplored., Objectives: To verify elevated CSF mtDNA concentrations in MS patients and assess the impact of aHSCT on mtDNA concentrations., Methods: Multiplex droplet digital PCR (ddPCR) was used to quantify mtDNA and nuclear DNA in 182 CSF samples. These samples were collected from 48 MS patients, both pre- and post-aHSCT, over annual follow-ups, and from 32 healthy controls., Results: CSF ccf-mtDNA levels were higher in patients with MS, correlated to multiple clinical and analytical factors and were normalized after intervention with aHSCT. Differences before aHSCT were observed with regard to MRI-lesions, prior treatment and number of relapses in the last year prior to aHSCT., Conclusion: Our findings demonstrate elevated CSF mtDNA levels in MS patients, which correlate with disease activity and normalize following aHSCT. These results position mtDNA as a potential biomarker for monitoring inflammatory activity and response to treatment in MS., Competing Interests: Declaration of competing interest The authors have no disclosures to report., (Copyright © 2024. Published by Elsevier B.V.)
- Published
- 2024
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