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1. Binding of calcium by fragment 38-108 of pike parvalbumin.

Author

Maximov EE and Mitin YV

Subjects
Amino Acid Sequence, Animals, Binding Sites, Chemical Phenomena, Chemistry, Circular Dichroism, Fishes, Kinetics, Peptide Fragments metabolism, Protein Binding, Calcium metabolism, Muscle Proteins, Parvalbumins
Abstract

Cyanogen bromide splitting of pike parvalbumin III was carried out and fragment 38-108 containing two calcium-binding domains was isolated. The obtained fragment binds calcium rather well (Kd = 1.6.10(-5) M), possesses a secondary structure, its CD spectrum changes after removal of calcium.

Published
1979
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