1. Somatodendritic Expression of JAM2 Inhibits Oligodendrocyte Myelination.
- Author
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Redmond SA, Mei F, Eshed-Eisenbach Y, Osso LA, Leshkowitz D, Shen YA, Kay JN, Aurrand-Lions M, Lyons DA, Peles E, and Chan JR
- Subjects
- Animals, Coculture Techniques, Junctional Adhesion Molecule B biosynthesis, Junctional Adhesion Molecule B genetics, Mice, Mice, Knockout, Myelin Sheath ultrastructure, Oligodendroglia ultrastructure, Primary Cell Culture, Rats, Spinal Cord physiology, Spinal Cord ultrastructure, Junctional Adhesion Molecule B physiology, Myelin Sheath metabolism, Oligodendroglia metabolism
- Abstract
Myelination occurs selectively around neuronal axons to increase the efficiency and velocity of action potentials. While oligodendrocytes are capable of myelinating permissive structures in the absence of molecular cues, structurally permissive neuronal somata and dendrites remain unmyelinated. Utilizing a purified spinal cord neuron-oligodendrocyte myelinating co-culture system, we demonstrate that disruption of dynamic neuron-oligodendrocyte signaling by chemical cross-linking results in aberrant myelination of the somatodendritic compartment of neurons. We hypothesize that an inhibitory somatodendritic cue is necessary to prevent non-axonal myelination. Using next-generation sequencing and candidate profiling, we identify neuronal junction adhesion molecule 2 (JAM2) as an inhibitory myelin-guidance molecule. Taken together, our results demonstrate that the somatodendritic compartment directly inhibits myelination and suggest a model in which broadly indiscriminate myelination is tailored by inhibitory signaling to meet local myelination requirements., (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Published
- 2016
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