9 results on '"Theilmeier, Gregor"'
Search Results
2. Perioperative levels and changes of high-sensitivity troponin T are associated with cardiovascular events in vascular surgery patients.
- Author
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Gillmann HJ, Meinders A, Grohennig A, Larmann J, Bünte C, Calmer S, Sahlmann B, Rustum S, Aper T, Lichtinghagen R, Koch A, Teebken OE, and Theilmeier G
- Subjects
- Aged, Aged, 80 and over, Female, Humans, Male, Middle Aged, Myocardial Infarction diagnosis, Myocardial Ischemia diagnosis, Perioperative Period, Predictive Value of Tests, Prospective Studies, ROC Curve, Regression Analysis, Risk Assessment, Myocardial Infarction etiology, Myocardial Ischemia etiology, Troponin T blood, Vascular Surgical Procedures adverse effects
- Abstract
Objectives: Myocardial infarction after major surgery is frequent, drives outcome, and consumes health resources. Specific prediction and detection of perioperative myocardial infarction is an unmet clinical need. With the widespread use of high-sensitive cardiac troponin T assays, positive tests become frequent, but their diagnostic or prognostic impact is arguable. We, therefore, studied the association of routinely determined pre- and postoperative high-sensitive cardiac troponin T with the occurrence of major adverse cardiac events., Design: This study was a prospective non-interventional trial., Setting: This study was conducted at Hannover Medical School in Germany., Patients: A total of 455 patients undergoing open vascular surgery were followed for 30 days for the occurrence of major adverse cardiac events., Interventions: None., Measurements and Main Results: Preoperative and 24-hour postoperative high-sensitive cardiac troponin T measurements and the respective changes were correlated to medical history and the occurrence of major adverse cardiac events (cardiovascular death, myocardial infarction, and ischemia). Pre- and postoperative high-sensitive cardiac troponin T measurements demonstrated a majority of patients with detectable troponin levels preoperatively and an increase over the 24 hours after surgery. The level of high-sensitive cardiac troponin T was significantly associated with preexisting diseases that constitute the Lee's Revised Cardiac Risk Index. A preoperative high-sensitive cardiac troponin T greater than or equal to 17.8 ng/L and a perioperative high-sensitive cardiac troponin T change greater than or equal to 6.3 ng/L are independently associated with the occurrence of major adverse cardiac events. Adding high-sensitive cardiac troponin T absolute change to the Revised Cardiac Risk Index improves the risk predictive accuracy of the score as evidenced by increased area under receiver operating characteristic and significant reclassification effects., Conclusions: The risk predictive power of high-sensitive cardiac troponin T change in addition to the Revised Cardiac Risk Index could facilitate 1) detection of patients at highest risk for perioperative myocardial ischemia, 2) evaluation and development of cardioprotective therapeutic strategies, and 3) decisions for admission to and discharge from high-density care units.
- Published
- 2014
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3. The receptor for activated complement factor 5 (C5aR) conveys myocardial ischemic damage by mediating neutrophil transmigration.
- Author
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Mueller M, Herzog C, Larmann J, Schmitz M, Hilfiker-Kleiner D, Gessner JE, and Theilmeier G
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- Animals, Cell Adhesion Molecules genetics, Cell Adhesion Molecules metabolism, Cell Movement genetics, Cells, Cultured, Gene Expression Regulation genetics, Humans, Matrix Metalloproteinase 9 genetics, Matrix Metalloproteinase 9 metabolism, Mice, Mice, Inbred C57BL, Mice, Knockout, Myocardial Infarction complications, Myocardial Reperfusion Injury etiology, Myocardium immunology, Myocardium pathology, Receptor, Anaphylatoxin C5a genetics, Receptor, Anaphylatoxin C5a immunology, Receptors, Cell Surface genetics, Receptors, Cell Surface metabolism, Transendothelial and Transepithelial Migration genetics, Tumor Necrosis Factor-alpha genetics, Tumor Necrosis Factor-alpha metabolism, Myocardial Infarction immunology, Myocardial Reperfusion Injury complications, Myocardium metabolism, Neutrophils immunology, Receptor, Anaphylatoxin C5a metabolism
- Abstract
Tissue loss after myocardial ischemia with reperfusion (MI/R) is in part conveyed by neutrophil recruitment to post-ischemic myocardium. Strategies to prevent reperfusion injury would help to limit myocardial damage. The receptor for activated complement factor 5 (C5aR) plays a prominent role in inflammation. We examine the effects of C5aR-deficiency on reperfusion injury after MI/R. C5aR(-/-)-mice and their C57BL/6- (WT) littermates underwent transient myocardial ischemia followed by different time points of reperfusion. Infarct size and leukocyte infiltration were determined. Expression of C5aR, inflammatory cytokines and adhesion molecules were analyzed by real-time RT-PCR. Leukocyte-endothelial interactions were assessed by low-shear adhesion- and transmigration-assays in vitro. Myocardial C5aR mRNA expression was 2.8-fold increased by ischemia. Infarct size per area-at-risk and leukocyte recruitment into infarctions were reduced in C5aR(-/-)-compared to WT-mice as well as in WT mice treated with the C5aR-antagonist JPE1375. IL-6, IL-1β, ICAM-1 and VCAM-1 expression were not different, while TNFα expression was reduced in C5aR(-/-)-mice after MI/R. In vitro, C5aR on leukocytes is required for effective transendothelial migration but not adhesion. Expression of MMP9 and JAM-A, molecules that are involved in leukocyte transmigration, were reduced in C5aR(-/-) mice in vivo. Genetic C5aR deficiency blunts the inflammatory response in murine MI/R resulting in reduced inflammatory cell recruitment, which is due to a C5aR-dependent effect on leukocyte transmigration across inflamed endothelium into the ischemic myocardium. This effect could be related to MMP9- and JAM-A expression in response to ischemia and reperfusion., (Copyright © 2013 Elsevier GmbH. All rights reserved.)
- Published
- 2013
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4. Continuous glycoprotein-130-mediated signal transducer and activator of transcription-3 activation promotes inflammation, left ventricular rupture, and adverse outcome in subacute myocardial infarction.
- Author
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Hilfiker-Kleiner D, Shukla P, Klein G, Schaefer A, Stapel B, Hoch M, Müller W, Scherr M, Theilmeier G, Ernst M, Hilfiker A, and Drexler H
- Subjects
- Animals, Cytokine Receptor gp130 genetics, Down-Regulation genetics, Humans, Interleukin-6 blood, Interleukin-6 genetics, Mice, Mice, Mutant Strains, Mutation, Missense, Myocardial Infarction genetics, Myocardial Infarction pathology, Myocarditis genetics, Myocarditis pathology, Myocytes, Cardiac metabolism, Myocytes, Cardiac pathology, Rupture, Spontaneous genetics, Rupture, Spontaneous metabolism, STAT3 Transcription Factor genetics, Cytokine Receptor gp130 metabolism, Myocardial Infarction metabolism, Myocarditis metabolism, STAT3 Transcription Factor metabolism
- Abstract
Background: In patients with myocardial infarction, high serum levels of interleukin-6 cytokines predict a poor outcome. The common receptor of interleukin-6 cytokines, glycoprotein-130 (gp130), signals via janus kinase/signal transducer and activator of transcription (STAT), cytoplasmic protein tyrosine phosphatase/extracellular signal-regulated kinase, and phosphoinositide-3-kinase/Akt pathways, and the regulation of these pathways depends at least in part on the gp130 tyrosine-757 residue. By analyzing cardiomyocyte-specific gp130(Y757F) mutant mice, we investigated the effect of disturbed gp130 signaling after myocardial infarction., Methods and Results: The cardiomyocyte-restricted alpha-myosin heavy chain-Cre-recombinase-loxP system was used to generate mice with gp130(Y757F) mutant cardiomyocytes (alphaMHC-Cre(tg/-);gp130(fl/Y757F) [Y(757)F]); all other cells carried at least 1 functional gp130 gene, ensuring normal gp130 signaling. Y(757)F mice displayed normal cardiac function and morphology at 3 months of age comparable to their nonmutant littermates. In response to myocardial infarction, Y(757)F mice displayed higher mortality associated with increased left ventricular rupture rate, sustained cardiac inflammation, and heart failure. These adverse effects were associated with prolonged and enhanced STAT3 activation and increased expression of interleukin-6 and of the complement-activating mannose-binding lectin C. Pharmacological inhibition of the complement system by cobra venom factor attenuated inflammation, prevented left ventricular rupture, and improved cardiac function in Y(757)F mice. Stronger effects were observed with a genetic reduction of STAT3 (STAT3(flox/+)) restricted to cardiomyocytes in Y(757)F mice, which prevented extensive upregulation of interleukin-6, complement activation, and sustained inflammation and lowered left ventricular rupture rate, heart failure, and mortality in subacute myocardial infarction., Conclusions: Impaired downregulation of gp130-mediated STAT3 activation in subacute infarction promotes cardiac inflammation, adverse remodeling, and heart failure, suggesting a potential causative role of high interleukin-6 serum levels after myocardial infarction.
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- 2010
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5. Accurate noninvasive measurement of infarct size in mice with high-resolution PET.
- Author
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Stegger L, Hoffmeier AN, Schäfers KP, Hermann S, Schober O, Schäfers MA, and Theilmeier G
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- Algorithms, Animals, Automation, Disease Models, Animal, Female, Fluorodeoxyglucose F18, Humans, Imaging, Three-Dimensional, Male, Mice, Myocardium pathology, Radiopharmaceuticals, Reproducibility of Results, Myocardial Infarction diagnosis, Myocardial Infarction pathology, Positron-Emission Tomography methods
- Abstract
Unlabelled: Reliable, repeatable, and time-efficient noninvasive measurement of infarct size in mice with PET would benefit studies aimed at the exploration of biochemical and functional changes associated with acute myocardial infarction (MI). PET with the radioactively labeled glucose derivative (18)F-FDG is used in humans to distinguish between viable but dysfunctional and nonviable myocardium. In this study, the feasibility, accuracy, and time efficiency of (18)F-FDG PET for quantification of infarct size in mice using a high-resolution animal PET device was evaluated in comparison with histomorphometry., Methods: Mice were subjected to surgery with permanent ligation of the left anterior descending artery. PET was performed before and 7 d after surgery. The infarct size was determined from the PET studies using both manual and automated delineation. The second PET scan was followed by histomorphometric analysis., Results: An excellent correlation between PET and histomorphometry was found for both manual (R = 0.98) and automated (R = 0.98) delineation, with linear regression curves close to unity (manual: y = 1.10x - 0.01; automated: y = 1.12x - 0.02). Automated analysis required <1 min per study., Conclusion: The measurement of infarct size in mice with (18)F-FDG PET is feasible and highly accurate. This noninvasive methodology permits unique longitudinal studies of biochemical parameters in mice and facilitates studies that aim to assess the effect of surgical and pharmacologic intervention after acute MI.
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- 2006
6. Adrenomedullin Is Associated With Surgical Trauma and Impaired Renal Function in Vascular Surgery Patients.
- Author
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Gillmann, Hans-Jörg, Meinders, Antje, Larmann, Jan, Sahlmann, Bianca, Schrimpf, Claudia, Aper, Thomas, Lichtinghagen, Ralf, Teebken, Omke E., and Theilmeier, Gregor
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ACUTE kidney failure ,CARDIOVASCULAR disease diagnosis ,SYSTEMIC inflammatory response syndrome ,TRAUMATOLOGY diagnosis ,ACADEMIC medical centers ,BIOMARKERS ,VASCULAR surgery ,CARDIOVASCULAR diseases risk factors ,LONGITUDINAL method ,PATIENTS ,PEPTIDE hormones ,STATISTICS ,SURGERY ,MULTIPLE regression analysis ,MEDICAL records ,PERIOPERATIVE care ,DIAGNOSIS ,DISEASE risk factors - Abstract
Background: Patients undergoing vascular surgery are prone to perioperative organ injury because of both higher prevalence of cardiovascular risk factors and the extent of surgery. Early detection of organ failure is essential to facilitate appropriate medical care. Midregional pro-adrenomedullin (MR-proADM) has been investigated in acute medical care settings to guide clinical decision-making regarding patient pathways and to identify patients prone to imminent cardiovascular or inflammatory complications. In this study, we evaluated the impact of perioperative MR-proADM levels as an early marker of perioperative cardiovascular and inflammatory stress reactions and kidney injury. Methods: The study was conducted as a monocentric, prospective, noninterventional trial at Hannover Medical School, Germany. A total of 454 consecutive patients who underwent open vascular surgery were followed from the day prior to until 30 days after surgery. The composite primary end point was defined as the occurrence of major adverse cardiac events (MACEs), acute kidney injury (AKI), or systemic inflammatory response syndrome (SIRS). Measurements were correlated with both medical history and postoperative MACE, AKI, or SIRS using univariate and multivariate regression analysis. Results: One hundred thirty-nine (31%) of the patients reached the primary end point within the study interval. Midregional pro-adrenomedullin change was associated with the combined primary end point and with the intensity of surgical trauma. Midregional pro-adrenomedullin change was increased in patients reaching the secondary end points, SIRS (optimal cutoff: 0.2 nmol/L) and AKI (optimal cutoff: 0.7 nmol/L), but not in patients with MACEs. Conclusion: Increased levels of MR-proADM within the perioperative setting (1) were linked to the invasiveness of surgery and (2) identified patients with ongoing loss of renal function. Increased MR-proADM levels may therefore identify a subgroup of patients prone to excessive cardiovascular stress but did not directly correlate with adverse cardiac events. Consistently low levels of MR-proADM may identify a subgroup of patients with acceptable low risk to guide discharge from high-density care units. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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7. Expression of fibulin-6 in failing hearts and its role for cardiac fibroblast migration.
- Author
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Chowdhury, Arpita, Herzog, Christine, Hasselbach, Lisa, Khouzani, Houra Loghmani, Zhang, Jinli, Hammerschmidt, Matthias, Rudat, Carsten, Kispert, Andreas, Gaestel, Matthias, Menon, Manoj B., Tudorache, Igor, Hilfiker-Kleiner, Denise, Mühlfeld, Christian, Schmitto, Jan Dieter, Müller, Martin, and Theilmeier, Gregor
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FIBULINS ,HEART failure ,FIBROBLASTS ,CELL migration ,GENE expression ,EXTRACELLULAR matrix ,MYOCARDIAL infarction - Abstract
Aims The cardiac extracellular matrix (ECM) undergoes a dynamic transition following myocardial infarction. Fibulin-6 is expressed in cell junctions particularly in tissues subjected to significant mechanical stress. Fibulin-6 deficiency results in defective cell migration in nematodes and early embryonic lethality in mice. The role of fibulin-6 in healthy and failing myocardium is unknown. We have examined the expression and distribution pattern of fibulin-6 during myocardial remodelling (MR) and detailed its effect on the migratory function of cardiac fibroblasts (CFs) in response to TGF-β1. Methods and results In healthy murine myocardium, fibulin-6 expression is largely confined to larger coronary arteries. It is induced during the early and the late phase of remodelling after infarction in murine hearts predominantly in the scar–muscle junction. Similar results are obtained in human ischaemic cardiomyopathy. Fibulin-6 is mostly expressed in close vicinity to vimentin-positive cells and is also abundantly expressed in vitro in cultured neonatal CF. TGF-β1 does not induce smooth muscle actin in fibroblasts deficient of fibulin-6, which also compromised their migration. Cells that had migrated expressed more fibulin-6 compared with stationary cells. Plated on fibulin-6-depleted matrix, stress fibre induction in fibroblast in response to TGF-β1 was impaired. In ex vivo explant cultures from post-infarct myocardium, the number of emigrating fibroblasts was also significantly reduced by fibulin-6 siRNA knockdown. Conclusion Fibulin-6, a fibroblast-released ECM protein, may play an important role during MR by imparting an effect on CF migration in close and complementary interplay with TGF-β1 signalling. [ABSTRACT FROM AUTHOR]
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- 2014
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8. Thrombomodulin's lectin-like domain reduces myocardial damage by interfering with HMGB1-mediated TLR2 signalling.
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Herzog, Christine, Lorenz, Anika, Gillmann, Hans-Jörg, Chowdhury, Arpita, Larmann, Jan, Harendza, Thomas, Echtermeyer, Frank, Müller, Martin, Schmitz, Martina, Stypmann, Jörg, Seidler, Daniela G., Damm, Martin, Stehr, Sebastian N., Koch, Thea, Wollert, Kai C., Conway, Edward M., and Theilmeier, Gregor
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THROMBOMODULIN ,LECTINS ,TOLL-like receptors ,MYOCARDIAL infarction ,ANTI-inflammatory agents ,CYTOKINES ,ISCHEMIA ,REPERFUSION ,HEART cells ,CELLULAR signal transduction - Abstract
Aims Thrombomodulin (TM), via its lectin-like domain (LLD), exhibits anti-inflammatory properties partly by sequestering the pro-inflammatory cytokine, high-mobility group box 1 (HMGB1). Since myocardial damage after ischaemia and reperfusion is mediated by inflammation, we evaluated the cardioprotective effects of the LLD of TM. Using an in vivo mouse model of transient ischaemia and in vitro models of cardiomyocyte hypoxia, we assessed the ability of the LLD to suppress HMGB1-mediated activation of the receptors, receptor for advanced glycation endproducts (RAGEs) and Toll-like receptors (TLRs) 2 and 4. Methods and results Thirty-minute myocardial ischaemia was induced in isoflurane-anaesthetized mice followed by 24 h of reperfusion in wild-type (WT) mice, in mice lacking the LLD of TM (TMLeD/LeD mice), and in WT with systemic overexpression of the LLD of TM induced by hydrodynamic transfection. Infarct size, HMGB1 protein, and apoptotic cells were significantly increased in TMLeD/LeD mice when compared with WT. Neonatal rat cardiomyocytes transfected with TLR2-, TLR4-, and RAGE-siRNA were exposed to hypoxia (0.8% O2) and reoxygenation (21% O2). HMGB1 augmented hypoxia-induced apoptosis in TLR2- but not in RAGE- or TLR4-suppressed cells. Administration of HMGB1- and TLR2-blocking antibodies in TMLeD/LeD mice prior to myocardial ischaemia diminished apoptosis. Therapeutic systemic gene therapy using the LLD reduced the infarct size and HMGB1 protein levels 24 h after reperfusion. Conclusion The LLD of TM suppresses HMGB1-induced and TLR2-mediated myocardial reperfusion injury and apoptosis in vitro and in vivo. [ABSTRACT FROM AUTHOR]
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- 2014
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9. Syndecan-4 signalling inhibits apoptosis and controls NFAT activity during myocardial damage and remodelling.
- Author
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Echtermeyer, Frank, Harendza, Thomas, Hubrich, Svenja, Lorenz, Anika, Herzog, Christine, Mueller, Martin, Schmitz, Martina, Grund, Andrea, Larmann, Jan, Stypmann, Jörg, Schieffer, Bernhard, Lichtinghagen, Ralf, Hilfiker-Kleiner, Denise, Wollert, Kai C., Heineke, Jörg, and Theilmeier, Gregor
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APOPTOSIS ,CELLULAR signal transduction ,VENTRICULAR remodeling ,MYOCARDIAL infarction ,LEFT heart ventricle ,HEART cells ,PROTEOGLYCANS ,MEMBRANE proteins - Abstract
Aims Myocardial infarction (MI) results in acute impairment of left ventricular (LV) function through the initial development of cardiomyocyte death and subsequent progression of LV remodelling. The expression of syndecan-4 (Sdc4), a transmembrane proteoglycan, is up-regulated after MI, but its function in the heart remains unknown. Here, we characterize the effects of Sdc4 deficiency in murine myocardial ischaemia and permanent infarction. Methods and results Targeted deletion of Sdc4 (Sdc4–/–) leads to increased myocardial damage after ischaemic–reperfusion injury due to enhanced cardiomyocyte apoptosis associated with reduced activation of extracellular signal-regulated kinase in cardiomyocytes in vitro and in vivo. After ischaemic–reperfusion injury and permanent infarction, we observed an increase in cardiomyocyte area, nuclear translocation of nuclear factor of activated T cells (NFAT), and transcription of the NFAT target rcan1.4 in wild-type mice. NFAT pathway activation was enhanced in Sdc4–/– mice. In line with the in vivo data, NFAT activation and hypertrophy occurs in isolated cardiomyocytes with reduced Sdc4 expression during phenylephrine stimulation in vitro. Despite the initially increased myocardial damage, echocardiography revealed improved LV geometry and function in Sdc4–/– mice 7 days after MI. Conclusion Interception of the Sdc4 pathway enhances infarct expansion and hypertrophic remodelling during early infarct healing in ischaemic–reperfusion injury and permanent infarction mouse models and exerts net beneficial effects on LV function. [ABSTRACT FROM PUBLISHER]
- Published
- 2011
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