1. Developmental changes in myocardial B cells mirror changes in B cells associated with different organs.
- Author
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Rocha-Resende C, Yang W, Li W, Kreisel D, Adamo L, and Mann DL
- Subjects
- Animals, Animals, Newborn, Antigens, CD metabolism, Female, Liver cytology, Liver growth & development, Lung cytology, Lung growth & development, Male, Mice, Inbred C57BL, Mice, Transgenic, Single-Cell Analysis, Spleen growth & development, Transcriptome, B-Lymphocytes physiology, Heart embryology, Heart growth & development, Myocardium cytology, Spleen cytology
- Abstract
The naive heart harbors a population of intravascular B cells that make close contact with the cardiac microvasculature. However, the timing of their appearance and their organ specificity remain unknown. To address this knowledge gap, we performed a systematic analysis of B cells isolated from the myocardium and other organs, from embryonic life to adulthood. We found that the phenotype of myocardial B cells changed dynamically during development. While neonatal heart B cells were mostly CD11b+ and CD11b- CD21-CD23-, adult B cells were predominantly CD11b-CD21+CD23+. Histological analysis and intravital microscopy of lung and liver showed that organ-associated B cells in contact with the microvascular endothelium were not specific to the heart. Flow cytometric analysis of perfused hearts, livers, lungs, and spleen showed that the dynamic changes in B cell subpopulations observed in the heart during development mirrored changes observed in the other organs. Single cell RNA sequencing (scRNAseq) analysis of B cells showed that myocardial B cells were part of a larger population of organ-associated B cells that had a distinct transcriptional profile. These findings broaden our understanding of the biology of myocardial-associated B cells and suggest that current models of the dynamics of naive B cells during development are incomplete.
- Published
- 2020
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