11 results on '"SABATINO, Maria Antonietta"'
Search Results
2. Facile crosslinking of poly(vinylpyrrolidone) by electro-oxidation with IrO2-based anode under potentiostatic conditions.
- Author
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Lanzalaco, Sonia, Sirés, Ignasi, Galia, Alessandro, Sabatino, Maria Antonietta, Dispenza, Clelia, and Scialdone, Onofrio
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CROSSLINKING (Polymerization) ,POVIDONE ,IRIDIUM oxide ,ELECTROCHEMICAL electrodes ,HYDROXYL group - Abstract
Abstract: The modification of polymer architectures by reaction with chemically adsorbed hydroxyl radicals has been thoroughly investigated by electrolyzing dilute aqueous solutions of the biocompatible polymer poly(vinylpyrrolidone) (PVP), using an undivided electrolytic cell with a Ti/IrO
2 -Ta2 O5 (DSA® ) anode. Several electrolyses were performed to assess the influence of the applied potential, the circulated charge and the PVP concentration, which was always kept low to avoid chain overlapping. From the results obtained, it can be concluded that the electro-oxidation of PVP solutions using a cheap anode is an effective method to crosslink initially isolated polymer chains, eventually increasing the size of their random coils. Furthermore, the average size of the modified macromolecules can be controlled by tuning the electrode potential and/or the current density and the circulated charge. At high anodic potential values, the hydroxyl radicals formed at DSA® were also effective to generate reactive functional groups on the PVP backbone, which is a very interesting feature for future biomedical applications.Graphical Abstract: [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
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3. E-Beam Crosslinked Polymeric and Bio-Hybrid Nanocarriers for Drug Delivery and Bioimaging
- Author
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GRIMALDI, Natascia, SABATINO, Maria Antonietta, ADAMO, Giorgia, GHERSI, Giulio, SPADARO, Giuseppe, DISPENZA, Clelia, Bulone, D, Grimaldi, N, Sabatino, MA, Bulone, D, Adamo, G, Ghersi, G, Spadaro, G, and Dispenza, C
- Subjects
PVP ,nanogel ,drug delivery ,bioimaging ,Settore CHIM/07 - Fondamenti Chimici Delle Tecnologie - Published
- 2014
4. Water-borne e-beam crosslinked nanogels as nanomaterials platform for drug delivery
- Author
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GRIMALDI, Natascia, SABATINO, Maria Antonietta, ADAMO, Giorgia, GHERSI, Giulio, SPADARO, Giuseppe, DISPENZA, Clelia, Bulone, D, Grimaldi, N, Sabatino, MA, Bulone, D, Adamo,G, Ghersi, G, Spadaro,G, and Dispenza, C
- Subjects
Nanogel ,drug delivery ,e-beam radiation - Published
- 2014
5. Radiation engineered polymer nanocarriers for a new generation of nanoparticles based tumor specific radiopharmaceuticals
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Clelia Dispenza, Sabatino, Maria Antonietta, Lorena Anna Ditta, Grimaldi, Natascia, Mangione, M., Adamo, G., Salvatrice Rigogliuso, Bulone, D., Giulio Ghersi, Jonsson, M., Dispenza, C, Sabatino, MA, Ditta, L, Grimaldi, N, Mangione, M, Adamo, G, Rigogliuso, S, Bulone, D, Ghersi, G, and Jonsson, M
- Subjects
radiation ,nanogel ,radiopharceuticals - Published
- 2013
6. Nose-to-brain delivery of insulin enhanced by a nanogel carrier.
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Picone, Pasquale, Sabatino, Maria Antonietta, Ditta, Lorena Anna, Amato, Antonella, San Biagio, Pier Luigi, Mulè, Flavia, Giacomazza, Daniela, Dispenza, Clelia, and Di Carlo, Marta
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NANOGELS , *TREATMENT of neurodegeneration , *BLOOD-brain barrier , *INSULIN therapy , *DRUG delivery systems - Abstract
Recent evidences suggest that insulin delivery to the brain can be an important pharmacological therapy for some neurodegenerative pathologies, including Alzheimer disease (AD). Due to the presence of the Blood Brain Barrier, a suitable carrier and an appropriate route of administration are required to increase the efficacy and safety of the treatment. Here, poly( N -vinyl pyrrolidone)-based nanogels (NG), synthetized by e-beam irradiation, alone and with covalently attached insulin (NG-In) were characterized for biocompatibility and brain delivery features in a mouse model. Preliminarily, the biodistribution of the “empty” nanocarrier after intraperitoneal (i.p.) injection was investigated by using a fluorescent-labeled NG. By fluorescence spectroscopy, SEM and dynamic light scattering analyses we established that urine clearance occurs in 24 h. Histological liver and kidneys inspections indicated that no morphological alterations of tissues occurred and no immunological response was activated after NG injection. Furthermore, after administration of the insulin-conjugated nanogels (NG-In) through the intranasal route (i.n.) no alteration or immunogenic response of the nasal mucosa was observed, suggesting that the formulation is well tolerated in mouse. Moreover, an enhancement of NG-In delivery to the different brain areas and of its biological activity, measured as Akt activation levels, with reference to free insulin administration was demonstrated. Taken together, these results indicate that the synthesized NG-In enhances brain insulin delivery upon i.n. administration and strongly encourage its further evaluation as therapeutic agent against some neurodegenerative diseases. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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- View/download PDF
7. E-beam crosslinked nanogels conjugated with monoclonal antibodies in targeting strategies.
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Adamo, Giorgia, Grimaldi, Natascia, Sabatino, Maria Antonietta, Walo, Marta, Dispenza, Clelia, and Ghersi, Giulio
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ELECTRON beams ,CROSSLINKED polymers ,BIOCONJUGATES ,MONOCLONAL antibodies ,TARGETED drug delivery ,CONFOCAL microscopy - Abstract
Poly(N-vinyl pyrrolidone)-based-nanogels (NGs), produced by e-beam irradiation, are conjugated with monoclonal antibodies (mAb) for active targeting purposes. The uptake of immuno-functionalized nanogels is tested in an endothelial cell line, ECV304, using confocal and epifluorescence microscopy. Intracellular localization studies reveal a faster uptake of the immuno-nanogel conjugate with respect to the 'bare' nanogel. The specific internalization pathway of these immuno-nanogels is clarified by selective endocytosis inhibition experiments, flow cytometry and confocal microscopy. Active targeting ability is also verified by conjugating a monoclonal antibody which recognizes the α
v β3 integrin on activated endothelial cells. Epifluorescence images of the 'wound healing assay' on ECV304 cells provide evidence of nanogels localization only in the target cells. Therefore, the immuno-nanogels produced have the potential to recognize specific cell types in heterogeneous systems, which makes them promising candidates for targeted drug delivery applications. [ABSTRACT FROM AUTHOR]- Published
- 2017
- Full Text
- View/download PDF
8. The role of molecular oxygen in the formation of radiation-engineered multifunctional nanogels.
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Ditta, Lorena Anna, Dahlgren, Björn, Sabatino, Maria Antonietta, Dispenza, Clelia, and Jonsson, Mats
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NANOGELS , *RADIATION chemistry , *WATER-soluble polymers , *SOLUTION (Chemistry) , *CHAIN scission , *POLYMER solutions - Abstract
• Radiation-engineered nanogels are a nanoplatform for drug delivery. • Experiments and kinetic modeling of radiation-induced synthesis of nanogels are combined. • A new code is developed for aqueous systems subjected to sequences of electron pulses. • Experimental and simulation results reveal the crucial role of transient O 2 on final polymer architecture. • Double bonds produced by disproportionation and scission reactions are quantified for the first time. Nanogels are very promising biomedical nanodevices. The classic "radiation chemistry-based" approach to synthetize nanogels consists in the irradiation with pulsed electron beams of dilute, N 2 O-saturated, aqueous solutions of water-soluble polymers of the "crosslinking type". Nanogels with controlled size and properties are produced in a single irradiation step with no recourse to initiators, organic solvents and surfactants. This paper combines experimental syntheses, performed with two e-beam irradiation setups and dose-ranges, starting from poly(N-vinyl pyrrolidone) solutions of various concentrations, both in N 2 O-saturated and air-saturated initial conditions, with the numerical simulations of the radiation chemistry of aqueous solutions of a radical scavanger exposed to the same irradiation conditions used in the experiments. This approach provides a methodology to predict the impact of system and irradiation conditions on the water radiation chemistry, which in turn affect the nanogel features in terms of molecular and physico-chemical properties. In particular, the crucial role of initial and transient concentration of molecular oxygen is revealed. This work also proposes a very simple and effective methodology to quantitatively measure the double bonds formed in the systems from disporportionation and chain scission reactions, competing with inter-/intra-molecular crosslinking. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
9. Crosslinking of poly(vinylpyrrolidone) activated by electrogenerated hydroxyl radicals: A first step towards a simple and cheap synthetic route of nanogel vectors.
- Author
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Galia, Alessandro, Lanzalaco, Sonia, Sabatino, Maria Antonietta, Dispenza, Clelia, Scialdone, Onofrio, and Sirés, Ignasi
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CROSSLINKING (Polymerization) , *POVIDONE , *ACTIVATED carbon , *NANOGELS , *HYDROXYL group , *DOPING agents (Chemistry) - Abstract
A facile electrosynthesis route for the preparation of polymer nanogels based on the in situ production of hydroxyl radicals is reported for the first time. Electro-Fenton process with continuous H 2 O 2 electrogeneration and Fe 2 + regeneration performs better than electro-oxidation with a boron-doped diamond or dimensionally stable anode for promoting crosslinking of poly(vinylpyrrolidone). [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
10. E-beam crosslinked nanogels conjugated with monoclonal antibodies in targeting strategies
- Author
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Natascia Grimaldi, Giulio Ghersi, Giorgia Adamo, Marta Walo, Maria Antonietta Sabatino, Clelia Dispenza, Adamo, Giorgia, Grimaldi, Natascia, Sabatino, Maria Antonietta, Walo, Marta, and Dispenza, Clelia, Ghersi, Giulio.
- Subjects
0301 basic medicine ,medicine.drug_class ,Confocal ,media_common.quotation_subject ,high-energy irradiation ,Clinical Biochemistry ,NG ,[object Object] ,02 engineering and technology ,Monoclonal antibody ,Biochemistry ,Cell Line ,Flow cytometry ,law.invention ,03 medical and health sciences ,Confocal microscopy ,law ,Fluorescence microscope ,medicine ,Humans ,Internalization ,Molecular Biology ,media_common ,radiation-engineered ,Drug Carriers ,medicine.diagnostic_test ,Chemistry ,wound healing assay ,antiβ3 integrin antibody ,Antibodies, Monoclonal ,Povidone ,active-targeting ,Biological Transport ,021001 nanoscience & nanotechnology ,Molecular biology ,Nanostructures ,030104 developmental biology ,Targeted drug delivery ,nanogel ,poly(N-vinyl pyrrolidone) ,Biophysics ,0210 nano-technology ,Gels ,Nanogel - Abstract
Poly(N-vinyl pyrrolidone)-based-nanogels (NGs), produced by e-beam irradiation, are conjugated with monoclonal antibodies (mAb) for active targeting purposes. The uptake of immuno-functionalized nanogels is tested in an endothelial cell line, ECV304, using confocal and epifluorescence microscopy. Intracellular localization studies reveal a faster uptake of the immuno-nanogel conjugate with respect to the ‘bare’ nanogel. The specific internalization pathway of these immuno-nanogels is clarified by selective endocytosis inhibition experiments, flow cytometry and confocal microscopy. Active targeting ability is also verified by conjugating a monoclonal antibody which recognizes the αvβ3 integrin on activated endothelial cells. Epifluorescence images of the ‘wound healing assay’ on ECV304 cells provide evidence of nanogels localization only in the target cells. Therefore, the immuno-nanogels produced have the potential to recognize specific cell types in heterogeneous systems, which makes them promising candidates for targeted drug delivery applications.
- Published
- 2016
11. A multifuctional nanoplatform for drug targeted delivery based on radiation-engineered nanogels
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Sabatino, Maria Antonietta, Ditta, Lorena Anna, Conigliaro, Alice, Dispenza, Clelia, Sabatino, Maria Antonietta, Ditta, Lorena Anna, Conigliaro, Alice, and Dispenza, Clelia
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COLON-CANCER CELLS ,PULSE-RADIOLYSIS ,Drug ,INDUCED CROSS-LINKING ,SPECTRAL PROPERTIES ,media_common.quotation_subject ,Nanogels ,Conjugation reactions ,Nanotechnology ,01 natural sciences ,AQUEOUS-SOLUTION ,FLUORESCEIN ,030218 nuclear medicine & medical imaging ,Nanogel ,03 medical and health sciences ,0302 clinical medicine ,Hydrophilic polymers ,0103 physical sciences ,NANOPARTICLES ,Molecule ,Ionising radiation synthesi ,IN-VIVO ,media_common ,chemistry.chemical_classification ,Radiation ,Aqueous solution ,010308 nuclear & particles physics ,Ionising radiation synthesis ,Rational design ,Polymer ,INSULIN ,Nanomedicine ,Conjugation reaction ,chemistry ,Drug delivery ,Settore CHIM/07 - Fondamenti Chimici Delle Tecnologie ,ACID) NANOGELS - Abstract
Under a rational design, combining biologically active molecules, ligands to specific cell receptors and fluorescent, radioactive or paramagnetic labels into a single nano-object can bridge the unique properties of the individual components and improve conventional sensing, imaging and therapeutic efficacies. The validation of these functional nano-objects requires careful testing both in terms of physico-chemical properties and biological behaviour in vitro and in vivo, prior to translation into the clinic. Ionising radiation of aqueous polymer solutions is a viable strategy to produce multifunctional nanogels from aqueous solutions of hydrophilic polymers. By proper selection of the irradiation conditions, polymer concentration and gaseous atmosphere, nanogels with the desired features in terms of dimensions, surface electric charge and chemical reactivity can be produced. In particular, radiation-engineered poly(N-vinyl pyrrolidone)-based nanogels bearing carboxyl groups and primary amines can be used as the main building block of promising theranostic nanodevices. The possibility of exploiting these functional groups to bind molecules of interest for their characterisation and biological evaluation is discussed.
- Published
- 2020
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