1. Formulation, Characterization and Biological Activity Screening of Sodium Alginate-Gum Arabic Nanoparticles Loaded with Curcumin.
- Author
-
Hassani A, Mahmood S, Enezei HH, Hussain SA, Hamad HA, Aldoghachi AF, Hagar A, Doolaanea AA, and Ibrahim WN
- Subjects
- Antioxidants pharmacology, Calorimetry, Differential Scanning, Cell Line, Tumor, Cell Survival drug effects, Curcumin toxicity, Humans, Microscopy, Electron, Transmission, Nanoparticles toxicity, Nanoparticles ultrastructure, Particle Size, Spectroscopy, Fourier Transform Infrared, X-Ray Diffraction, Alginates chemistry, Antineoplastic Agents pharmacology, Cell Proliferation drug effects, Curcumin pharmacology, Drug Carriers chemistry, Gum Arabic chemistry, Nanoparticles chemistry
- Abstract
The approach of drug delivery systems emphasizes the use of nanoparticles as a vehicle, offering the optional property of delivering drugs as a single dose rather than in multiple doses. The current study aims to improve antioxidant and drug release properties of curcumin loaded gum Arabic-sodium alginate nanoparticles (Cur/ALG-GANPs). The Cur/ALG-GANPs were prepared using the ionotropic gelation technique and further subjected to physico-chemical characterization using attenuated total reflectance-Fourier transform infrared (ATR-FTIR), X-ray diffractometry (XRD), differential scanning calorimetry (DSC), size distribution, and transmission electron microscopy (TEM). The size of Cur/ALG-GANPs ranged between 10 ± 0.3 nm and 190 ± 0.1 nm and the zeta potential was -15 ± 0.2 mV. The antioxidant study of Cur/ALG-GANPs exhibited effective radical scavenging capacity for 1,1-diphenyl-2-picrylhydrazyl (DPPH) at concentrations that ranged between 30 and 500µg/mL. Cytotoxicity was performed using MTT assay to measure their potential in inhibiting the cell growth and the result demonstrated a significant anticancer activity of Cur/ALG-GANPs against human liver cancer cells (HepG2) than in colon cancer (HT29), lung cancer (A549) and breast cancer (MCF7) cells. Thus, this study indicates that Cur/ALG-GANPs have promising anticancer properties that might aid in future cancer therapy.
- Published
- 2020
- Full Text
- View/download PDF