1. Design of Ultrasound-Driven Charge Interference Therapy for Wound Infection.
- Author
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Zhou J, Ji X, Wang H, Hsu JC, Hua C, Yang X, Liu Z, Guo H, Huang Y, Li Y, Cai W, Lin X, and Ni D
- Subjects
- Animals, Mice, Ultrasonic Waves, Reactive Oxygen Species metabolism, Wound Healing drug effects, Humans, Porphyrins chemistry, Porphyrins pharmacology, Porphyrins therapeutic use, Ultrasonic Therapy methods, Gram-Positive Bacteria drug effects, Gram-Negative Bacteria drug effects, Anti-Bacterial Agents pharmacology, Anti-Bacterial Agents chemistry, Anti-Bacterial Agents therapeutic use, Wound Infection drug therapy, Wound Infection microbiology, Nanoparticles chemistry, Nanoparticles therapeutic use, Biofilms drug effects
- Abstract
Wound infections, especially those caused by pathogenic bacteria, present a considerable public health concern due to associated complications and poor therapeutic outcomes. Herein, we developed antibacterial nanoparticles, namely, PGTP, by coordinating guanidine derivatives with a porphyrin-based sonosensitizer. The synthesized PGTP nanoparticles, characterized by their strong positive charge, effectively disrupted the bacterial biosynthesis process through charge interference, demonstrating efficacy against both Gram-negative and Gram-positive bacteria. Additionally, PGTP nanoparticles generated reactive oxygen species under ultrasound stimulation, resulting in the disruption of biofilm integrity and efficient elimination of pathogens. RNA-seq analysis unveiled the detailed mechanism of wound healing, revealing that PGTP nanoparticles, when coupled with ultrasound, impair bacterial metabolism by interfering with the synthesis and transcription of amino acids. This study presents a novel approach to combatting wound infections through ultrasound-driven charge-interfering therapy, facilitated by advanced antibacterial nanomaterials.
- Published
- 2024
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