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Your search keyword '"Young-Shik Yun"' showing total 2 results
Start Over Author "Young-Shik Yun" Topic nanopillar
2 results on '"Young-Shik Yun"'

1. Stem cell behaviors on periodic arrays of nanopillars analyzed by high-resolution scanning electron microscope images

Author

Jihun Kang, Eun-Hye Kang, Young-Shik Yun, Seungmuk Ji, In-Sik Yun, and Jong-Souk Yeo

Subjects
Cell behavior, Human adipose-derived stem cell, Scanning electron microscope, Polyurethane acrylate, Nanopillar, Microscopy, QH201-278.5
Abstract

Abstract The biocompatible polyurethane acrylate (PUA) nanopillars were fabricated by soft lithography using three different sizes of nanobeads (350, 500, and 1000 nm), and the human adipose-derived stem cells (hASCs) were cultured on the nanopillars. The hASCs and their various behaviors, such as cytoplasmic projections, migration, and morphology, were observed by high resolution images using a scanning electron microscope (SEM). With the accurate analysis by SEM for the controlled sizes of nanopillars, the deflections are observed at pillars fabricated with 350- and 500-nm nanobeads. These high-resolution images could offer crucial information to elucidate the complicated correlations between nanopillars and the cells, such as morphology and cytoplasmic projections.

Published
2020
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2. Quantitative Correlation of Nanotopography with Cell Spreading via Focal Adhesions Using Adipose-Derived Stem Cells

Author

Seungmuk Ji, Yong Oock Kim, Young Shik Yun, Jong-Souk Yeo, Eun Hye Kang, In Sik Yun, and Su Bong Lee

Subjects
Cell, Biomedical Engineering, Filamentous actin, General Biochemistry, Genetics and Molecular Biology, Biomaterials, Focal adhesion, Cell Movement, medicine, Adipocytes, Cell Adhesion, Humans, Nanotopography, Nanopillar, Fluorocarbons, Focal Adhesions, biology, Tissue Engineering, Chemistry, Stem Cells, Fatty Acids, Cell Differentiation, Adhesion, Vinculin, Silanes, Nanostructures, medicine.anatomical_structure, Adipose Tissue, biology.protein, Biophysics, Stem cell
Abstract

Nanotopography mimicking extracellular environments reportedly impact cell morphological changes; however, elucidating this relationship has been challenging. To control cellular responses using nanostructures, in this study, the quantitative relationship between nanotopography and cell spreading mediated by focal adhesions (FAs) is demonstrated using adipose-derived stem cells (ASCs). The spreading of ASCs and area of FAs are analyzed for the distribution of filamentous actin and vinculin, respectively, using fluorescent images. FAs require a specific area for adhesion (herein defined as effective contact area [ECA]) to maintain cell attachment on nanopillar arrays. An ECA is the area of FAs supported by nanopillars, multiplying the area fraction (AF) of their top surface. Regarding the spreading of cells, the mean area of ASCs linearly decreases as the mean area of FAs increases. Because the area of FAs is inversely correlated to the AF of the nanopillar arrays, the spreading of cells can be quantitatively correlated with nanotopography. The results provide a conceptual framework for controlling cell behaviors to design artificial substrates for tissue-engineering applications.

Published
2020

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