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The anomalous Nernst effect (ANE), as a three-dimensional thermoelectric effect in ferromagnetic materials, is expected to break through the limitations of the traditional Seebeck effect, thereby improving thermoelectric conversion efficiency. This study reports an anisotropic anomalous Nernst effect in metallic nickel assembled by the alignment of nanowires. It is found that the ANE voltage varies with the angle to the alignment direction of nanowires within the in-plane of metallic nickel. The ANE voltage reaches 530 nV under a longitudinal temperature difference of 23 K when the magnetic field is applied parallel to the alignment direction of nanowires, which is 143% of its value in the vertical direction. This design concept for anisotropic materials may provide a novel path to enhanced thermoelectric efficiency in ANE-based thermoelectric devices. [ABSTRACT FROM AUTHOR]