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Populations inhabiting the periphery of species distribution ranges may experience suboptimal environmental conditions and higher vulnerability to anthropogenic pressures. Disentangling the role of natural and human-related factors and the relationships among them in these marginal areas is thus key to understand and prevent species declines and range reductions. We analysed Eurasian otter (Lutra lutra) occurrence patterns in relation to anthropogenic pressures and natural environmental gradients in Morocco, an area constituting the arid limit of the species’ global range. The probability of otter occurrence was higher as terrain ruggedness increased and at intermediate elevations, and lower in catchments exposed to higher anthropogenic pressures. Otters tended to be rare at higher elevations and in areas with less annual precipitation, probably as a result of trophic resource limitations and large water flow fluctuations, respectively. A combination of natural and anthropogenic factors, both at drainage area and local scales, was needed to understand the current occurrence of the species. Our study highlights a need for urgent action to conserve the Eurasian otter in northern Africa, where freshwater ecosystems and their associated biodiversity are threatened by rapid human development in areas of marginal climatic conditions., We acknowledge the help of our colleagues in conducting the field sampling: Begoña Adrados, Seila Alvaré, Manuela González, Carlos Gutiérrez-Expósito, Miguel Jácome, Francisco Palomares, Carli Pérez, Eloy Revilla, Jacinto Román and Pablo Villalba, and the scientific advice of Abdeljebbar Qninba and his help in processing the permits necessary to conduct the study. We thank three anonymous referees for their constructive comments on an earlier manuscript. The picture of an otter used in Figure 4 was donated by Juan Matutano. Sampling permissions were obtained from the Haut Commissariat aux Eaux et Forêts et a la Lutte contre la Désertification du Royaume du Maroc. Financial support was received from Estación Biológica de Doñana-CSIC. N.F. acknowledges the support of iDiv funded by the German Research Foundation (DFG–FZT 118, 202548816). Funding for Open Access charge: Universidad de Huelva / CBUA.

10 pages, 1 table, 2 figures.-- Open access, Public surveys can be valuable tools to collect information on wildlife distribution. Our objective was to improve the knowledge on the distribution and expanding areas of the Egyptian mongoose (Herpestes ichneumon) in central Spain by using an on-line questionnaire to hunters. We assessed the reliability of information that hunters provided by comparing it with reports from wildlife professionals. Our results show a high overall congruence but significantly different information provided by hunters and professionals on the distribution of the Egyptian mongoose, suggesting that both sources provide unique data for the management of non-game species. For example, hunters reported greater mongoose presence than professionals in areas to the east of Toledo and Ciudad Real provinces, for the whole distribution and core area of the species, suggesting that hunters could help to detect other areas where the species exists. Overall, our findings stress the importance of engaging hunters in wildlife conservation, as they can share valuable information on wildlife species, including those that are not hunted, E. Descalzo was supported by a predoctoral research contract funded by the University of Castilla-La Mancha co-supported by the Plus European Social Fund (FSE+). F. Díaz-Ruiz was supported by a postdoctoral research contract funded by the University of Málaga through the grant program Ayudas para la Incorporación de Doctores del I Plan Propio de Investigación de la Universidad de Málaga (Call 2020). This study is part of the research project SBPLY/17/180501/000184, funded by the regional Government of CLM and the European Regional Development Fund

Our moral circles—that is, the entities believed as worthy of moral concern and thus deserving moral considerations—have historically expanded beyond humans to include also nonhuman beings (Crimston et al., 2018). As a result, various emerging legal instruments around the world have been granting rights to animals. However, the inclusion of animals within moral circles is subjected to important biases, with a preference for charismatic, familiar, and beautiful vertebrates (Klebl et al., 2021). We argue that legal instruments embracing such biases may jeopardize biodiversity conservation., The authors would like to thank all researchers who have kindly contributed to the discussion of this conservation problem in Spain.

Montane biota is vulnerable to climate change, especially in the case of relict species in environmentally extreme areas. The Dades trout Salmo multipunctata is a relict species from the Draa basin, on the southern slopes of the High Atlas Mountains, Morocco. Apart from its genetic and morphological singularity almost nothing is known about this species. We surveyed the whole potential distribution range of the Dades trout and found that only two isolated populations exist (in the Dades and M'Goun catchments), occupying an extremely small range, < 22 km of stream reaches in a narrow altitudinal range (c. 2,150–2,375 m). The species was found more frequently and more abundantly at intermediate elevations within its range, and somatic condition increased with altitude. Climatically suitable areas for the Dades trout will be confined to mountain summits without permanent water bodies by 2070. The Dades trout is a Critically Endangered species in need of active management for its persistence. We propose actions for the long-term conservation of the species, including catchment-scale erosion control, riverbed restoration, local-scale measures to mitigate global warming, and an ex situ breeding programme.

The European rabbit Oryctolagus cuniculus faces a paradoxical situation in its native range on the Iberian Peninsula. While many populations have declined sharply due to a new variant of the rabbit haemorrhagic disease virus (RHDV-b), others remain healthy. The latter populations, which flourish mostly on farmland, cause significant crop damage. We explored if this difference could be related to the existence of the 2 rabbit subspecies (O. c. algirus and O. c. cuniculus) that coexist allopatrically on the Iberian Peninsula. Potential differences in population trends between rabbit subspecies may also be relevant in assisting the conservation of endangered rabbit-dependent predators which mainly occur in the distribution area of O. c. algirus. To test this, we assessed rabbit trends after the outbreak of RHDV-b by an online questionnaire to the senior administrative officers of all provincial official game departments throughout peninsular Spain (n = 47). A generalized negative trend was reported by officers in the distribution area of O. c. algirus, while a more stable or even positive trend was reported in the distribution area of O. c. cuniculus. We point to the need for establishing a long-term rabbit population monitoring programme on the Iberian Peninsula to further confirm the observed patterns, but also to contribute to evidence-based management decision-making. Our results suggest a need to apply different management systems for each rabbit subspecies., P.H.V. was supported by a grant (FPU17/04821) from the Spanish Ministry of Science, Innovation and Universities. This study was partially funded by Projects 201810I026 and 202010E004 financed by CSIC.

Conservation conflicts arise frequently in the management of non-native invasive species (NIS) when such species provide socioeconomic benefits and have negative environmental impacts (van Wilgen & Richardson 2014). For example, when restrictions on exploitation of NIS are applied in response to demands from the public or agencies, NIS users may believe conservation interests take precedence over their interests (Fig. 1). These restrictions are justified by the benefits to biodiversity, but the absence of such benefits may generate a loss of social support for conservation, potentially leading to deregulation. To illustrate, we considered management of the red swamp crayfish in Spain and the European rabbit in New Zealand.

The recreational use of natural resources requires the implementation of sustainable management systems. However, the existence of socioeconomic interests and the difficulty involved in applying evidence-based criteria often hinder this implementation. The European wild rabbit (Oryctolagus cuniculus) is an appropriate case with which to study the recreational hunting governance systems. This species has, in recent decades, undergone important population changes in its native area, the Iberian Peninsula, where it plays a triple role as a game, pest and key ecological species. The rabbit is consequently intensively managed for hunting purposes, for the conservation of rabbit-dependent predators and in order to mitigate damage to crops. In this paper, we study the governance system of rabbit hunting through the use of questionnaire surveys distributed to managers working for Spanish regional governments. We repeated the survey in 2001 and 2016 in order to evaluate how the system had evolved over a 15-year period of important changes in rabbit population abundance. We found two different responses to these changes. The growth of rabbit populations in provinces in which rabbits cause crop damage in farmland areas has been dealt with an increase in hunting pressure, derived from both administrative decisions and hunters’ management. However, in provinces where rabbit populations have sharply declined in natural vegetation (non-farmland) areas, the management seems to be driven mostly by inertia, with a high influence of tradition and little use of evidence on administrative decisions. Hunters in these provinces have changed their management practices to little or no extent, where even the high importance of rabbit hunting and its (moderate) economic importance do not appear to provide an incentive for the change required., This study is a contribution of the CGL2013-43197-R project (I + D National Plan), funded by the Spanish Ministry of Science and Innovation.

The Egyptian mongoose (Herpestes ichneumon L.) is a medium-size carnivore widely distributed in Africa and in a small part of southern Europe, the Iberian Peninsula, where mongoose populations have recently expanded. The mongoose is relatively easily detectable because of its diurnal habits and because it is the only species of Herpestidae occurring in the Iberian Peninsula. Therefore, its distribution could be monitored through citizen science. In this sense, information provided by stakeholders that make frequent use of natural environments, including hunters, landowners, or wildlife rangers, would be potentially very valuable. Nevertheless, the accuracy of the information provided by these stakeholders as regards mongoose occurrence has never been tested. To do so, we compared mongoose occurrences gathered through field transects (i.e., 2-km walking surveys in which direct observations and indirect signs were recorded) carried out in 218 Andalusian municipalities during 2010–2015 with those obtained through questionnaires conducted in 2016 to hunters (n = 251), landowners (n = 116), and wildlife rangers (n = 133). We did not find any significant difference between mongoose distribution estimated by the reference method (i.e., field surveys) and by questionnaire to wildlife rangers. In contrast, mongoose occurrences reported by hunters and landowners were significantly correlated among them, but not with those collected in field transects (nor with those provided by the rangers). This suggests that a participatory network for monitoring mongoose distribution could rely on the information provided by wildlife rangers. Previous studies showed that hunters can provide useful information from less accessible areas like private estates where official data are not collected. In this sense, our results suggest that further effort is needed to incorporate hunters and landowners in a participatory network to monitor mongoose distribution, and this could include collaborative actions to promote their involvement in addition to increasing their skills in mongoose detection., This study is framed within the projects INIA-CON15-189, RTI2018-096348-RC21, and SBPLY/17/180501/000184.

The extraordinary population growth of certain ungulate species is increasingly a concern in agroforestry areas because overabundance may negatively affect natural environments and human livelihoods. However, society may have negative perceptions of killing wildlife to reduce their numbers and mitigate damage. We used an online survey that included a choice experiment to determine Spanish citizens’ (n = 190) preferences toward wildlife population control measures related to negative effects of ungulate overabundance (negative impacts on vegetation and other wildlife species and disease transmission to livestock) in 2 agroforestry national parks in Spain. We used latent-class and willingness-to-pay in space models to analyze survey results. Two percent of respondents thought a national park should have no human intervention even if lack of management may cause environmental degradation, whereas 95% of respondents favored efforts to reduce damage caused by overabundant ungulate species. We estimated human well-being losses of survey respondents when sustainable effects of deer overabundance on the environment became unsustainable effects and well-being gains when sustainable effects transitioned to no visible effects. We found that the type of wildlife-control program was a very relevant issue for the respondents; indirect control in which killing was avoided was the preferred action. Sixty-six percent of respondents agreed with the option of hunters paying for culling animals to reduce ungulate impacts rather than management cost coming out of taxes, whereas 19% of respondents were against this option and willing to pay for other solutions in national parks. Our results suggest that killing wildlife in national parks could be a socially acceptable tool to manage overabundance problems in certain contexts, but it could also generate social conflicts., This study is framed within the project Social and Ecological Insights for the Sustainable Management of Game Species in Agroforestry Landscapes: from Overabundant to Declining Species” (ref. RTI 2018‐096348‐R‐C21) funded by the Spanish Ministry of Science, Innovation, and Universities.

Cage-trapping is one of the control methods frequently used by hunters to remove red foxes (Vulpes vulpes) in Spain, although its low efficiency and selectivity have been frequently reported. We tested the effect of the combinations of two bait types (live/ dead) and two scent attractants (fox urine/valerian extract) in order to improve the efficiency and selectivity of cage traps in central Spain. The combination of live bait and fox urine increased the efficiency of cage traps to capture red foxes in comparison with live bait alone. None of the experimental treatments led to a significant reduction in the capture rate of non-Target species, selectivity levels were always low (0%-21%) and non-Target species were captured. In view of that, the burden of releasing captured non-Target animals depends ultimately on the trapper. For this reason, the use of cage traps is still questionable for managing foxes in Spain., Castilla-La Mancha Regional Government; code numbers 02-227/RN-52 and PREG-05-23.

The collection of biological information, including data gathered in the field, is fundamental to improve our understanding of how human impacts on biological systems can be recognized, mitigated or averted. However, the role of empirical field research has faded appreciably in the past decades with sobering implications. Indeed, important instruments to help set national and global priorities in biodiversity conservation (i.e. synthetic analyses and big data approaches) can be severely handicapped by a lack of sound observational data, collected through fieldwork. We analyzed publication trends in the conservation literature from 1980 to 2014 to ascertain whether there is reason for concern about a potential decrease in fieldwork-based investigations compared to other types of studies. Here, we show that the proportion of fieldwork-based investigations in the conservation literature dropped significantly from the 1980s until today; indeed, fieldworkbased publications decreased by 20% in comparison to a rise of 600% and 800% in modelling and data analysis studies, respectively. In parallel, we found that the most highly cited academic journals in conservation science published fieldwork studies less frequently than the lower rank journals. We contend that an apparent decrease in fieldwork-based investigations is the result of bottom-up pressures, including those associated with the publishing and the academic reward systems, while a second set acts top-down, driven by current societal needs and/or priorities. We urge researchers, funders and journals to commit, respectively, to conducting, funding and divulging relevant fieldwork research, and make some recommendations on specific steps that can be adopted in that direction. V Plan Propio de Investigación de la Universidad de Sevilla Marie Curie PIOF-GA-2013-621571

In Europe, hunting and game management are centuries-old activities that have had profound effects on the landscapes and the biodiversity they support. Game management is a potentially important tool to enhance game populations and may also affect other wildlife. We investigated the relationship between red-legged partridge Alectoris rufa game management and steppe-bird abundance in central Spain. We surveyed 54 red-legged partridge hunting estates with varying game-management intensity in spring and/or summer in 2006 and 2008–2010. Information about game management was gathered through questionnaires to game managers. Birds were counted from fixed points and the amount of different land uses in each point was visually estimated. Our results show that the abundance of granivorous species (sandgrouse) increased significantly with the density of feeders, whereas non-granivorous species were more abundant in estates with more intensive fox control, although the latter relationship was weaker. Land-use variables, specifically agricultural lands, appeared in the models of all the groups considered. Therefore, there is an option for designing optimal management for red-legged partridge estates that potentially benefits non-target species: an effective combination of habitat management, adequate use of supplementary food and water, and possibly selective legal predator control when necessary and effective for the target game species, although the latter needs further investigation.

[Question] Anthropogenic edges caused by transport infrastructure such as dirt roads and trails (also known as Soft Linear Developments; SLD) are pervasive in almost every terrestrial ecosystem. Revegetating these edges may reduce some of their negative effects, such as their permeability to biological invasions and detrimental effects on wildlife, potentially becoming suitable habitat for a broad range of species. Selecting species with low post-dispersal seed predation rates may improve the effectiveness of revegetation programmes., [Location] Mediterranean scrublands in SW Spain. [Methods] We made offerings of a total of 16,000 seeds of eight species of fleshy-fruit shrubs both along SLD edges and scrubland interiors in two independent blocks in each of three distant locations. Using four types of selective enclosure, we assessed the relative contribution of three seed predator guilds (ants, rodents and birds) to seed predation rates both along SLD edges and scrubland interiors. [Results] The effects of anthropogenic edges on seed predation rates were species-specific. The large and hard-seeded species Chamaerops humilis was not predated at all. Juniperus phoenicea and Corema album seeds had higher predation rates in scrubland interiors than in edges. The small-seeded Rubus ulmifolius experienced relatively low seed predation rates compared to the other species. Predation rates for this species were higher along SLD edges than in scrubland interiors. Ants were the main seed predators in the area, and showed marked preferences for J. macrocarpa and C. album seeds at both SLD edges and scrubland interiors. [Conclusions] Our results show the strong context-dependency of seed predation rates in both SLD edges and scrubland interiors, and thus the importance of well spatially and temporally replicated studies. Species with large and hard seeds may be good candidates for roadside revegetation programmes. However, the relative suitability of plant species would depend on the seed predator community. Our findings confirm that studies on seed predation may help planning cost-effective species selection for edge revegetation efforts worldwide., This research was financed by the Spanish Ministerio de Medio Ambiente, Rural y Marino (070/2009), Ministerio de Ciencia e Innovación (CGL2010‐21926/BOS) and Ministerio de Educación (FPU grant to A.S.E.; AP2008‐01874).

We explored the views held by Spanish hunters on hunting, and assessed how these views varied according to participant characteristics and whether or not the responses were made public via a hunter’s magazine. Hunters expressed many positive values in relation to hunting, but were critical of their own community as well as other stakeholders. Spanish hunters felt misunderstood and even attacked by society at large. There was also a widespread negative perception of the future of hunting. The frequency of references to different opinions varied between anonymous and public respondents and in relation to age group. Participants who expressed their opinions publicly mentioned the ecological values of hunting more frequently, were more critical towards their own community, and viewed the future of hunting more positively than anonymous participants. This may indicate an intended projected positive image; namely, Spanish hunters may publicly emphasize those views that improve their position in society., This study was initiated as part of HUNT-Hunting for Sustainability project (http://fp7hunt.net/), which was funded by the European Union’s Framework Programme 7; was partially funded by Junta de Comunidades de Castilla-La Mancha through project PPII-2014-016-A; and contributes to PIE 201330E105 (CSIC). M. Delibes-Mateos is currently funded by V Plan Propio de Investigación of the Universidad de Sevilla (Spain).

Desert rivers are highly dynamic systems in which dry periods, frequently involving the cessation of superficial flow, alternate with violent flash floods. In spite of their territorial importance and high functional and biological diversity, desert rivers have received little attention in the scientific literature, especially in Northern Africa. This study investigated the distribution of non-native fish, their relationship with river damming and their potential impacts on native biodiversity in the main river basins draining arid areas of Morocco (Oued Draa, Oued Ziz and Oued Ghir), based on field sampling covering 84 stream reaches. Thirteen fish species were recorded, eight of which are non-native. Two species (stone moroko, Pseudorasbora parva, and bleak, Alburnus alburnus) had not been previously recorded in Morocco, while the native sandsmelt (Atherina boyeri) had not been cited as an invader. Pumpkinseed (Lepomis gibbosus) was the most widely distributed non-native fish. Non-native fish species were associated with reservoirs, colonizing the regulated downstream reaches. In contrast, unregulated upstream reaches tended to be free of non-natives. The low abundance of native fish in reservoirs seems to be caused by the impacts of introduced fish species. Management options for environmental flow are limited, owing to reduced water availability. Non-native fish management should thus focus on limiting the spread of species already introduced and avoiding new introductions, especially into reservoirs. Copyright © 2014 John Wiley & Sons, Ltd.

In southern Europe, traditional hunting has been frequently replaced by models based on more intensive management. These systems include management strategies like the release of farm-reared animals that can cause harmful effects on biodiversity. However, little is known about the hunters’ views of this activity, and about their preferences for the ecological attributes of the hunting estates. We present the results of a choice experiment exercise evaluating the willingness to pay of Spanish hunters regarding different aspects of walked-up red-legged partridge (Alectoris rufa) shooting, including partridge quality (farm-reared vs. wild) and other attributes related to the ecological characteristics of the estate. We find that, when given the choice, hunting an additional wild partridge in a walked-up shooting day was valued more than 20 times higher than hunting an additional farm-reared bird. The diversity of small game available and the presence of natural vegetation in the landscape in which the walked-up hunting takes place were also significantly valued. Hunters also attributed economic value (albeit lower than other attributes) to the presence of protected non-game fauna in the estate. Overall, our results show that hunters are willing to pay more for hunting on estates that have better ecological characteristics, which may be indicative of good conservation status. This suggests that identifying and promoting such estates could lead to systems that are both ecologically and economically sustainable., This work was supported by the European Commission under the 7th FWP for Research and Technological Development, project HUNT (212160, FP7-ENV-2007-1). M. D-M. is currently supported by a JAE-DOC contract (Programa Junta para la Ampliación de Estudios), funded by CSIC and the European Social Fund (ESF). J.C. had a postdoctoral contract jointly funded by the ESF and by the Junta de Comunidades de Castilla–La Mancha (Spain), Operational Programme FSE 2007–2013.

The red-legged partridge (Alectoris rufa) is the most important gamebird in Spain. Due to population declines in recent decades, management approaches increasingly include the release of farm-reared partridges and the establishment of so-called intensive estates, where hunting is based on put-and-take partridges. The release of farm-reared partridges is controversial because these birds negatively affect wild populations. However, according to managers, releases are necessary to maintain commercial hunting. We analysed economic parameters using data provided by 20 red-legged partridge hunting estates with different management and economic aims in central Spain. We calculated total revenues, total expenses, benefits and profitability of these estates and simulated results under two different scenarios (prices for buying farm-reared partridges being 15 % higher and prices for hunting in estates not using releases being 15 % higher) attempting to mimic the internalization of some ecological costs of farm-reared birds or possible rewards from using wild populations sustainably. Non-commercial estates (n = 5), as expected, just broke even, with almost no benefits. Benefits of intensive estates, where massive releases throughout the hunting season allow a much higher harvest, were on average an order of magnitude higher (20,404 ± 24,413 €/km, n = 8) than those of other commercial estates (1713 ± 1280 €/km, n = 7). Benefits of non-intensive commercial estates using releases were on average lower than those of commercial estates without releases. In fact, profitability of non-intensive commercial estates not using releases was similar to that of intensive estates. Any of the two price scenarios considered led to non-intensive commercial estates not using releases being the most profitable on average. Thus, efforts should be made to identify ways of improving economic profitability of non-intensive estates, which may be important for promoting hunting management styles that are ecologically more beneficial., Work was supported by the European Commission (7th Framework Programme for R&D through project HUNT, 212160, FP7-ENV-2007-1), JCCM through project ‘Efecto del medio natural, las prácticas agrícolas, ganaderas y de gestión cinegética sobre las poblaciones de caza menor en los cotos pertenecientes a la Asociación de cotos de caza Sierra de Alcaraz Campo de Montiel’, and by CSIC (PIE 201330E105). S. Díaz Fernández had a predoctoral grant funded by the European Social Fund (ESF) and by JCCM (Operational Programme PRINCET 2005-2010). M. Delibes-Mateos is supported by the V Plan Propio de Investigación of the University of Seville.

Behavioral effects of living under predation risk may influence the dynamics of prey species more than direct demographic effects. Human recreation, especially hunting, can also force prey to increase their vigilance and can influence distribution and habitat use even more than natural predators. However, behavioral effects do not play a prominent role in conservation or wildlife management. Whereas the demographic consequences of hunting are documented for large carnivores, behavioral effects of hunting on their dynamics remain unexplored. We studied the movement patterns of brown bears (Ursus arctos) in Scandinavia as a model species, before and after the start of the annual bear hunting season. Bears were expected to become more active at daytime as the season progressed due to shortening daylight. How- ever, the start of hunting disrupted this pattern. Solitary bears subject to hunting increased movements during the dark hours after hunting started, losing their nocturnal rest, probably to compensate for decreased daytime activity. Females with cubs-of-the-year, which are protected from hunting, also mod- ified their movement pattern, but much less than hunted bears. Bears altered their movement pattern at a critical time of the year, during hyperphagia, when they must store fat reserves before hibernation, which is critical for reproduction. Behavioral effects of hunting should be a relevant issue for the conser- vation and management of large carnivores, especially when hunting occurs during highly sensitive peri- ods of the year. This concern applies to many species managed under hunting regimes

Theory suggests that demographic and genetic traits deteriorate (i.e., fitness and genetic diversity decrease) when populations become small, and that such deterioration could precipitate positive feedback loops called extinction vortices. We examined whether demographic attributes and genetic traits have changed over time in one of the 2 remaining small populations of the highly endangered Iberian lynx (Lynx pardinus) in Donana, Spain. From 1983 to 2008, we recorded nontraumatic mortality rates, litter size, offspring survival, age at territory acquisition, and sex ratio. We combined these demographic attributes with measures of inbreeding and genetic diversity at neutral loci (microsatellites) and genes subjected to selection (major histocompatibility complex). Data on demographic traits were obtained through capture and radio tracking, checking dens during breeding, track surveys, and camera trapping. For genetic analyses, we obtained blood or tissue samples from captured or necropsied individuals or from museum specimens. Over time a female-biased sex ratio developed, age of territory acquisition decreased, mean litter size decreased, and rates of nontraumatic mortality increased, but there were no significant changes in overall mortality rates, standardized individual heterozygosity declined steadily, and allelic diversity of exon 2 of class II major histocompatibility complex DRB genes remained constant (2 allelic variants present in all individuals analyzed). Changes in sex ratio and age of territory acquisition may have resulted from demographic stochasticity, whereas changes in litter size and nontraumatic mortality may be related to observed increases in inbreeding. Concomitant deterioration of both demographic attributes and genetic traits is consistent with an extinction vortex. The co-occurrence, with or without interaction, of demographic and genetic deterioration may explain the lack of success of conservation efforts with the Donana population of Iberian lynx.

The first clear evidence of the establishment of an invasive freshwater bivalve in Africa is provided, through the description of the distribution of Corbicula fluminea in Morocco. C. fluminea was detected in 2008 and 2011 in two independent river basins, although still absent in other river systems throughout the country. This information is used to propose management actions, which should be focused on avoiding interbasin expansion as well as new introduction events from European fresh waters. Copyright © 2012 John Wiley & Sons, Ltd.

Hyperpredation can be described as a restrictive case of apparent competition where an increased number of primary prey species indirectly induces the decrease of the secondary prey species through numerical response of predators to the primary prey dynamics. It has been proposed that rabbit hemorrhagic disease (RHD), which decimated populations of European wild rabbit (Oryctolagus cuniculus) in Spain, led to prey switching by raptors towards red-legged partridges (Alectoris rufa) causing declines in their populations as a peculiar case of hyperpredation. We have reviewed field studies that disagree with estimates (based on bag records) of recent increases of rabbit and partridge numbers in Spain. Because of an increase in releases of farm-reared animals, there is doubt about the use of bag records to accurately estimate population trends in recent decades. We also provide new data and discuss some analytical considerations related to the temporal and spatial scales that might affect the interpretation of data. Finally, we discuss why studies associated with raptor food habits, predator population dynamics and predator distribution suggest that diet data are not sufficient to link patterns and processes. Although we agree that the RHD outbreak has markedly affected rabbit populations, which has indirectly affected many other species in the Iberian Peninsula, we consider that hyperpredation mediated by raptors has not been clearly demonstrated. In contrast, endorsing the hypothesis of raptor-mediated hyperpredation without sufficient proof may have conflictive consequences if we consider the increased persecution of raptors in recent decades in Spain., J.A. Blanco-Aguiar was supported by Fundação para a Ciência e a Tecnologia (SFRH/BPD/65464/2009) grant. M. Delibes-Mateos was supported by a Juan de la Cierva research contract awarded by the Ministerio de Ciencia e Innovación—Fondo European Social Fund. F. Casas was supported by a postdoctoral grant of the Junta de Comunidades de Castilla la Mancha (JCCM).

The European rabbit Oryctolagus cuniculus is a key species in Mediterranean ecosystems of the Iberian Peninsula, and reliable methods for monitoring its abundance are urgently required. Although clearance plot pellet counts may be useful in monitoring rabbit populations, such counts are potentially affected by variation in the persistence of rabbit pellets. The aim of our study was to assess persistence of rabbit pellets in the Iberian Peninsula for reliable estimates of rabbit abundance from clearance plot counts. We carried out the experiment from July 2006 to November 2007 at seven localities in central southern Spain. Marked fresh pellets were monthly placed at experimental plots, and their persistence was monitored monthly. Persistence was estimated from the proportion of marked pellets remaining between consecutive counts. Independent variables in analysis included slope steepness, vegetation, rabbit and ungulate activity, meteorology and temporal variables. Persistence significantly varied among localities and seasons and with their interaction. The final model showed that pellet persistence was best explained by total rainfall between counts, and also included the time between visits and slope steepness and the interactions rainfall*time between visits and rainfall*slope. Our results suggest that estimating pellet persistence for each locality and season is necessary to make estimates obtained from different localities and months comparable. In the Mediterranean region, early summer at the start of the dry season would be the recommended time for yearly rabbit monitoring based on any pellet-count index, since reduced rainfall favours pellet persistence. Sampling protocols must standardise the number of days between counts, and plots must be established in areas with gentle slope to maximise pellet persistence between counts., Our work is a partial result from the I+D National Plan Projects CGL2005-02340 and CGL2009-10741, funded by the Spanish Ministry of Science and Innovation and EU-FEDER funds. J. Fernandez-de-Simon benefits from a FPI scholarship funded by the Spanish Ministry of Science and Innovation and the European Social Fund. M. Delibes-Mateos is currently holding a Juan de la Cierva research contract awarded by the Spanish Ministry of Science and Innovation and the European Social Fund.

Small mammals, such as European rabbits (Oryctolagus cuniculus), plateau pikas (Ochotona curzoniae) and prairie dogs (Cynomys spp.), traditionally have been perceived as pests and targeted for control within their native ranges, where they perform essential ecosystem roles and are considered keystone species. These species can reach high densities, and have been subjected to eradication campaigns because of their putative negative impact on natural habitats and agriculture and their competition with livestock for forage. Eradication programmes have been a main factor causing sharp declines of these species in their natural ranges. Paradoxically, they are keystone species where they are abundant enough to be perceived as pests. The term > pest> is usually a social perception that is rarely supported by scientific data, whereas there is considerable scientific evidence of the key ecological roles played by these species. We call for the conservation of these species and present a conceptual model regarding the management of their populations. Where they occur at high numbers, and hence their effects on biodiversity are still of crucial importance, the persecution of these species should be avoided and their natural habitats preserved. In areas with high conservation value, but where these species occur at low densities, management efforts should aim to increase their density. In areas of high commercial value, managers ideally should consider changing prioritization of the area to high conservation value by purchasing the land or obtaining conservation easements. In situations with high commercial value and demonstrable low conservation concern, small mammals could be reduced humanely., M.D.M. was supported by a postdoctoral grant funded by the regional government of Castilla-La Mancha (JCCM) and the European Social Fund, and is currently holding a Juan de la Cierva research contract awarded by the Ministerio de Ciencia e Innovación and the European Social Fund. This study was born during a sabbatical stay of J.E.S. at the Doñana Biological Station of the CSIC, with financial support of the Consejeria de Innovacion, Ciencia y Empresa of the Junta de Andalucia. A.T.S. acknowledges the support of the US NSF Dynamics of Coupled Natural and Human Systems Program (DBCS-0814794).

European rabbits (Oryctolagus cuniculus), a multifunctional keystone species in the Iberian Peninsula, have drastically declined over past decades. Rabbit decline has been frequently attributed to the arrival of two viral diseases. However, decline was apparently ongoing before the arrival of the diseases, as a consequence of habitat loss and fragmentation. In this paper, the effect on rabbit populations of land-use changes during recent decades in Andalusia (southern Spain) is analysed. Areas favourable for rabbits both at present and during the 1960s are identified, and the environmental and land-use factors that determine these areas established. In areas where the favourability for rabbits has changed during recent decades, main land use changes are assessed to identify possible factors explaining rabbit favourability in these areas. Areas favourable to rabbits are currently determined by factors similar to those during the 1960s; these areas have undergone geographic changes in recent decades, apparently as a consequence of land-use changes in Andalusia. The percentages of the variables that were positively associated with rabbit favourability in both models (current and 1960s) have declined in Andalusia as a whole, and in areas where rabbit favourability has decreased; hence environments suitable for rabbits have become impoverished. Conversely, in both models, environments suitable for rabbits increased in municipalities, where rabbit favourability also increased. The preservation of rabbit-friendly habitats should be a priority for the conservation of this key species in the western Mediterranean., M. Delibes-Mateos was supported by a postdoctoral grant 469 from the regional government of Castilla la Mancha (JCCM) 470 and the European Social Fund (ESF).

Understanding the factors underlying species coexistence is a major focus in community ecology. When dealing with competition between native and exotic species, the competitive exclusion of the ‘weaker’ species can have consequences for biodiversity conservation. We examined interspecific interactions during foraging between black vultures Coragyps atratus and Andean condors Vultur gryphus in Southern Argentina. Previous studies suggest facilitatory interactions between them. However, in most parts of their distribution ranges, these species did not coexist until recently, when black vultures expanded their geographic range following human development. Thus, facilitatory processes or segregation patterns could not be fine-tuned enough to allow their coexistence. Our hypothesis is that black vultures and Andean condors compete for food resources, and that this competitive scenario can change depending on local species abundances and habitat humanization. We experimentally placed sheep carcasses in two habitats differing in degrees of humanization to study the foraging patterns in these species. Both species exploited carcasses with similar temporal patterns. However, the first to arrive and the dominant species were different in both habitats, according to their abundances. Although black vultures do not completely prevent the arrival of Andean condors to carcasses, they represent serious obstacles for feeding. Thus, while dominance hierarchy at carcasses could be related to body size, carcass consumption was determined by species abundance. Our results support the hypothesis that the expansion of a ‘winning’ species may trigger interspecific competition with other ‘loser’ species, with negative responses towards human activities when they became abundant. Although the results are not conclusive, invasion and extinction processes could be occurring and they can have serious consequences for the diversity (i.e. richness and evenness) of the New World scavenger guilds.

European rabbits (Oryctolagus cuniculus), a keystone species in the Iberian Mediterranean ecosystem, are the staple prey of the Iberian lynx (Lynx pardinus) and the Spanish imperial eagle (Aquila adalberti). These predators require medium to high rabbit densities and a low degree of human disturbance. We compared rabbit abundances in areas of central-southern Spain under three levels of protection and management: protected areas, intensively managed (nonprotected) hunting estates, and other nonprotected areas. We used pellet abundance indices to estimate rabbit density in 118 surveys conducted during the summers of 2002 and 2003. We observed greater rabbit abundance in intensively managed hunting estates compared to protected areas and other nonprotected areas, perhaps because policy makers did not consider rabbit numbers when selecting priority areas. Alternatively, differences in game management practices (e.g., predator control or habitat management) may explain the higher rabbit densities observed in managed hunting estates. Our results suggest that the best feeding conditions for the Iberian lynx and the Spanish imperial eagle occur in intensively managed hunting areas, where such predators are frequently persecuted. The conservation of these endangered predators may require efforts to increase rabbit densities in protected areas., The first author was supported by a I3P grant funded by the European Social Fund through the “Consejo Superior de Investigaciones Científicas” (CSIC). Funding was provided by the projects CGL 2005-02340/BOS co-funded by the Spanish Ministry of Education and Science and European Regional Development Fund, and PAI06-170 by the regional government of Castilla-La Mancha.

The Iberian Lynx Conservation Breeding Program follows a multidisciplinary approach, integrated within the National Strategy for the Conservation of the Iberian lynx, which is carried out in cooperation with national, regional and international institutions. The main goals of the ex situ conservation programme are to: (1) maintain a genetically and demographically managed captive population; (2) create new Iberian lynx Lynx pardinus free-ranging populations through re-introduction. To achieve the first goal, the Conservation Breeding Program aims to maintain 85% of the genetic diversity presently found in the wild for the next 30 years. This requires developing and maintaining 60–70 Iberian lynx as breeding stock. Growth projections indicate that the ex situ programme should achieve such a population target by the year 2010. Once this goal is reached, re-introduction efforts could begin. Thus, current ex situ efforts focus on producing psychologically and physically sound captive-born individuals. To achieve this goal, we use management and research techniques that rely on multidisciplinary input and knowledge generated on species' life history, behaviour, nutrition, veterinary and health aspects, genetics, reproductive physiology, endocrinology and ecology. Particularly important is adapting our husbandry schemes based on research data to promote natural behaviours in captivity (hunting, territoriality, social interactions) and a stress-free environment that is conducive to natural reproduction.

[Context] Seed-dispersing animals often move along “linear gaps” (linear anthropogenic features such as roads and trails that contain little vegetation), especially in densely-vegetated landscapes. As a result, linear gaps and their verges may receive more seeds than adjacent habitats. In addition, linear gap verges may provide more suitable conditions for plant establishment than neighboring habitats. In this way, linear gaps may increase plant abundance and diversity, and facilitate connectivity of native and non-native plant populations, ultimately increasing plant diversity in the landscape., [Objectives] We reviewed current evidence for the potential of anthropogenic linear gaps to increase plant abundance and diversity, and for the mechanisms involved., [Methods] We reviewed peer-reviewed literature published up to December 31st, 2014., [Results] Most (69.2 %) studies found significantly higher plant abundance and/or diversity in linear gap verges than in adjacent habitats. This suggests that linear gaps can increase plant abundance and diversity, and possibly facilitate population spread. However, there was a strong bias toward the study of exotic species. In addition, there were few mechanistic studies to allow estimation of the relative contributions of dispersal and post-dispersal mechanisms operating in linear gaps., [Conclusions] Future studies should focus on entire plant communities, not just exotic species, and should allow identification of the mechanisms by which linear gaps increase plant abundance and diversity. With this knowledge in hand, we will be in a better position to understand whether the net benefit of linear gaps for plant diversity in general outweigh their facilitation of the spread of exotic species., This research was financed by the Spanish Ministerio de Educación (FPU grant to A.S.E.; AP2008-01874).

Throughout Europe, increased levels of land abandonment lead to (re)colonization of old lands by forests and shrublands. Very little is known about the spatial pattern of plants recolonizing such old fields. We mapped in two 21–22-ha plots, located in the Doñana National Park (Spain), all adult individuals of the endozoochorous dwarf palm Chamaerops humilis L. and determined their sex and sizes. We used techniques of spatial point pattern analysis (SPPA) to precisely quantify the spatial structure of these C. humilis populations. The objective was to identify potential processes generating the patterns and their likely consequences on palm reproductive success. We used (1) Thomas point process models to describe the clustering of the populations, (2) random labeling to test the sexual spatial segregation, and (3) mark correlation functions to assess spatial structure in plant sizes. Plants in both plots showed two critical scales of clustering, with small clusters of a radius of 2.8–4 m nested within large clusters with 38–44 m radius. Additional to the clustered individuals, 11% and 27% of all C. humilis individuals belonged to a random pattern that was independently superimposed to the clustered pattern. The complex spatial pattern of C. humilis could be explained by the effect of different seed-dispersers and predators’ behavior and their relative abundances. Plant sexes had no spatial segregation. Plant sizes showed a spatial aggregation inside the clusters, with a decreasing correlation with distance. Clustering of C. humilis is strongly reliant on its seed dispersers and stressful environmental conditions. However, it seems that the spatial patterns and dispersal strategies of the dwarf palm make it a successful plant for new habitat colonization. Our results provide new information on the colonization ability of C. humilis and can help to develop management strategies to recover plant populations

A keystone species is one that is crucial in maintaining the organization and diversity of their ecological communities. We tested the idea that rabbits act as a keystone species in southern Europe by exploring relationships between rabbit abundance and the abundance and diversity of raptor species. At 20 sites in southern Spain we assessed rabbit abundance through counts of animals along transects and assessed the number of raptor individuals and species through watches from vantage points. In a further 120 locations we also derived an index of rabbit abundance, from pellets and compared this to the presence or absence of the critically endangered Spanish imperial eagle. Rabbit abundance was posi- tively associated with the number of observations of raptors, the number of raptor species and the number of species of conservation concern. Sites with the most rabbits had higher conservation value. Moreover, the presence of Spanish imperial eagle was strongly associ- ated with sites where rabbits were at high density. We conclude that rabbits do act as a key- stone species and we suggest that conservation efforts should focus on improving the status of this small-game species in southern Europe.

We evaluated the existence of trophic guild struc- ture, considering seasonal and annual variation, in two terrestrial carnivore assemblages: one from Santa Cruz province (Argentinean Patagonia, composed by six carni- vore species), and the other from Doñana National Park (SW Spain, composed by five carnivore species). To identify trophic guilds, we first studied seasonal and annual diets of predators, calculated trophic overlap among species pairs, and then constructed overlap matrices (similarity matrices). We determined guild membership objectively by entering the similarity matrices into the clustering technique un- weighted pair-group method with arithmetic averaging. Carnivores from both assemblages were grouped, respec- tively, into four feeding guilds. Lagomorphs and rodents promoted the formation of two feeding guilds in both study sites, although the taxonomic composition of predator species that composed them was different. The ungulates- edentates feeding guild was only present at Santa Cruz, whereas the birds and reptiles feeding guild was only present at Doñana. Invertebrates and fruits were the base for the formation of a guild composed by species of the same taxonomic origin both in Santa Cruz and Doñana. Guild structure of Santa Cruz and Doñana assemblages did not exhibit seasonal or annual variation, although the specific guild composition changed over the two studied periods for both assemblages. This structure probably responded to discontinuities in resource spectra in Santa Cruz and fluctuations in rabbit abundance in Doñana. Our results support the hypothesis that establishes that guilds are originated by opportunistic convergence of species on abundant and energetically rewarding resources

The release of farm-reared animals for shooting causes frequent conflicts between hunters and conservationists, since, while this management practice is economically important in some game areas, it carries several risks for biodiversity conservation (e.g. the introduction of new pathogens or the release of alien species and/or hybrids). However, these conflicts have received little attention in the literature. In particular, social-psychological factors, which are very important driving conservation conflicts, have been often ignored in the study of releases. Our main goal was to examine attitudes and beliefs of game managers towards the release of farm-reared red-legged partridges in small-game estates within central Spain, where more than 3 million partridges are released annually. Data were collected through face to face interviews with 45 game managers. More than 70% of the interviewed managers expressed negative views towards releases, and these included arguments about their detrimental effect on natural partridge populations, their low effectiveness, and their consideration as artificial hunting. Very negative views predominated among managers who had never released partridges (mostly those of non-commercial estates), and were frequently expressed by those who released partridges occasionally. In contrast, positive views were mostly given by managers who released partridges annually, and arguments used generally referred to the economic benefits of releases. Some managers expressed at the same time both positive and negative views on releases (i.e. ambivalent position). These findings suggest that there exists a relative polarisation among game managers, and that the position of those who were very critical of releases is close to that of conservationists. Our results also suggest that managers’ decision-making regarding releases is likely influenced by a variety of beliefs and attitudes as well as the socioeconomic setting (e.g. economic interest in the outcome of the behaviour). This highlights that the study of different aspects, including social and psychological as well as economic, is essential for understanding and resolving conservation conflicts, such as those caused by releases., This work was supported by the European Commission (7th Framework Programme for R&D through project HUNT, 212160, FP7-ENV-2007-1), by CSIC (PIE 201330E105), and by Junta de Comunidades de Castilla-La Mancha (PPII-2014-016-A). M. Delibes-Mateos is currently funded by Consejería de Economía, Innovación, Ciencia y Empleo of Junta de Andalucía, and the European Union's Seventh Framework Programme for research, technological development and demonstration under grant agreement 267226.

Knowledge about how hunting pressure is determined, and the relative efficacy of different mechanisms to regulate harvest, can help to improve the managers’ decision-making process. We developed a general framework about the decision-making process that regulates red-legged partridge (Alectoris rufa) hunting pressure in central Spain based on information from a focus group and individual interviews with game managers. We also used available information to compare the efficiency of different tools thus improving some decision steps. We evaluated the cost-effectiveness of different population monitoring methods as a way to reduce uncertainty on partridge availability to hunters. Additionally, we investigated the relationship between annual harvest and various regulatory mechanisms of partridge hunting pressure used in the study area to identify the most potentially useful one to limit annual take-off. Game managers usually set hunting pressure after a qualitative assessment on population abundance prior to the hunting season, but this decision was frequently modified during the course of the hunting season according to variations in catch or perceived abundance at that time. Our results showed that kilometric abundance indices (counting partridges from cars along line transects) was a simple cost-efficient and reliable estimate of partridge density (estimated by Distance sampling). A variety of regulatory mechanisms were used by managers. The variables that most affected annual harvest (in addition to partridge abundance) were the number of driven-shooting days, and hunter density in walked-up hunting days, suggesting that their adjustment will be the most efficient regulatory mechanisms. We conclude that adequate monitoring on population abundance should be a critical step for managers’ decision-making, and that a better understanding of the relative value of regulatory mechanisms, combining social and ecological approaches, would help improving our understanding of any human-mediated system, thus leading to better management recommendations., Work was supported by the European Commission (7th Framework Programme for R&D through project HUNT, 212160, FP7-ENV-2007-1); Consejería de Agricultura of JCCM; by the Ministerio de Ciencia y Tecnología (CGL2008-04282/BOS), and by CSIC (PIE 201330E105).

[EN] We developed a conceptual framework for classifying habitat quality that requires the construc- tion of separate habitat models for each key demographic feature; the framework can be applied when the factors that determine different demographic processes differ substantially. For example, survival of large car- nivores is mainly determined by human-induced mortality, whereas nutritional condition determines repro- ductive rate. Hence, a two-dimensional habitat model built for reproduction and survival yields five hypothet- ical habitat categories: matrix, with no reproduction and/or very high mortality; sink, with low reproduction and high mortality; refuge, with low reproduction and low mortality; attractive sink, with high reproduction and high mortality; and source, with high reproduction and low mortality. We applied this framework to two endangered brown bear ( Ursus arctos ) populations in the Cantabrian Mountains, Spain. Our aim was to gen- erate working hypotheses about the quality and spatial arrangement of bear habitat to analyze the present conditions of the different population nuclei and to facilitate identification of core areas of high conservation value, conflictive areas, or areas with unoccupied potential habitat. We used a geographic information system and two spatial long-term data sets on presence and reproduction and performed logistic regressions for building a two-dimensional model. The analysis reveals that both populations exist under different subopti- mal conditions: the eastern population mainly occupies areas of suboptimal natural habitat and relatively low human impact, whereas the western population is located mainly in areas with high human impact but otherwise good natural quality. To test hypotheses about demographic features of the obtained habitat cate- gories, we classified data on historic extinction in northern Spain ( fourteenth to nineteenth centuries ) with the two-dimensional model. Extinction probabilities within each habitat category confirmed the hypotheses: most extinctions occurred in matrix habitat, and the fewest occurred in source habitat., [ES] Desarrollamos un marco conceptual para clasificar la calidad del hábitat que requiere la con- strucción de modelos de hábitat separados para cada característica demográfica clave. El marco se puede aplicar cuando difieren sustancialmente los factores que determinan diferentes procesos demográficos. Por ejemplo, la supervivencia de grandes carnívoros esta determinada principalmente por mortalidad inducida por humanos, mientras que la condición nutricional determina la tasa reproductiva. Consecuentemente, un modelo bidimensional del hábitat construido para reproducción y supervivencia produce cinco categorías hipotéticas de hábitat: matriz ( sin reproducción y/o mortalidad muy alta ), sumidero ( reproducción baja, mortalidad alta ), refugio ( reproducción baja, mortalidad baja ), sumidero atractivo ( reproducción alta, mor- talidad alta ) y fuente ( reproducción alta, mortalidad baja ). Aplicamos este marco a dos poblaciones en peli- gro de oso pardo ( Ursus arctos ) en la Cordillera Cantábrice, España. Nuestra meta fue generar hipótesis de trabajo sobre la calidad y la distribución espacial del hábitat de los osos para analizar las condiciones actuales de los diferentes núcleos de población, para facilitar la identificación de áreas núcleo de alto valor de conservación, áreas conflictivas o áreas con hábitat potencial desocupado. Utilizamos un sistema de infor- mación geográfica y dos conjuntos de datos históricos de presencia y reproducción y aplicamos regresiones logísticas para construir un modelo bidimensional. El análisis revela que ambas poblaciones existen bajo diferentes condiciones sub-óptimas: la población oriental ocupa principalmente áreas de hábitat natural sub- óptimo y relativamente bajo impacto humano, mientras que la población occidental se localiza principal- mente en áreas con alto impacto humano, pero por lo demás con buena calidad natural. Para evaluar las hipótesis sobre las características demográficas de cada tipo de hábitat, clasificamos datos de extinciones históricas en el norte de España ( siglos XIV a XIX ) con el modelo bidimensional. Las probabilidades de extin- ción en cada categoría de hábitat confirmaron las hipótesis: la mayoría de las extinciones ocurrieron en hábitat matriz y la minoría ocurrió en hábitat fuente.

We studied the relationship between extinction frequencies of Iberian lynx subpopulations (Lynx pardinus) and their size and isolation during a 35-year period of strong geographic range contraction. At the end of this period there were fewer fragmentation events, fewer lynx populations of small size, and less isolation between them, than in simulated geographic ranges derived from a random distribution of local extinctions. Only small populations occupying < 500 km2 went extinct. Local extinction in large, self- sustainable populations probably resulted from the sole action of deterministic factors, e.g. widespread prey decline. As compared with large populations, small ones experienced increased contraction per unit occupied area, which may reflect demographic unstability. The consistent effect of isolation on extinction suggests that such unstability was often prompted by reduced immigra- tion and the subsequent disruption of metapopulation equilibrium. Several practical recommendations could be derived. Provided that habitat quality was adequate, a lynx population should avoid extinction within 35 years if it occupied an area of at least 500 km2. The persistence of small populations will also be enhanced by minimizing the distances to neighbouring populations within 30 km, and by maximizing the area occupied by these neighbours. Therefore, habitat management, or any other restoration action, directed to expand the area occupied by a small lynx population should be best located at points of its boundaries oriented towards other existing nearby populations.

[Capsule]: Feeding habits of the Black-billed Magpie are of interest for researchers, conservationists and hunters since magpies are considered as predators of eggs and chicks of both songbirds and gamebirds. [Aims]: To characterize the feeding habits of magpies during their breeding season in agricultural environments of central Spain, and to assess the occurrence and incidence of birds and eggs in the magpie's diet. [Methods]: Diet was determined by the analysis of gizzard contents from 118 culled magpies. The diet was described as the frequency of occurrence (FO) and the percentage of volume (VOL) of a certain food item and for each gizzard. [Results]: Magpies presented a generalist diet, which included a wide range of foods. Arthropods and cereal seeds were the most frequently consumed food groups (FO > 60%). Eggs and birds were consumed only occasionally (FO < 6% and 17%, respectively; percentage of volume, VOL < 4%). [Conclusion]: Our findings suggest that other birds and their eggs do not represent an important food for magpies in Mediterranean agricultural environments under the conditions found during this study. Nevertheless, more complex studies in different scenarios (i.e. different population sizes of magpies and prey), and over longer temporal scales, are still necessary to clarify this controversial issue., The study was funded by Consejería de Medio Ambiente of Junta de Comunidades de Castilla-La Mancha (Project PREG-05-23). F. Díaz-Ruiz holds a postdoctoral contract financed by the European Social Fund (ESF) and the Junta de Comunidades de Castilla-La Mancha (Operational Programme FSE 2007/2013). M. Delibes-Mateos is supported by the Talentia Postdoc Program launched by the Andalusian Knowledge Agency, co-funded by the European Union’s Seventh Framework Program, Marie Skłodowska-Curie actions (COFUND – Grant Agreement n° 267226) and the Ministry of Economy, Innovation, Science and Employment of the Junta de Andalucía.

Hunting and its associated management have significant costs and benefits for biodiversity conservation,which makes this socio-economic activity highly controversial at both international and regional levels. We investigated relationships between management for small game species (mainly Red-legged Partridges Alectoris rufa and rabbits Oryctolagus cuniculus ) and both abundance and richness of farmland and scrubland songbirds, raptors and ground-nesting birds, and on the abundance of three species of conservation concern (Little Bustard Tetrax tetrax , Eurasian Thick-knee Burhinus oedicnemus and Montagu’s Harrier Circus pygargus ) in southern Portugal farmland. We compared 12 game estates and 12 matching areas with similar sizes and land uses but no game management. Richness and abundance were estimated from fixed point counts, and were related to game regime (managed or unmanaged), habitat characteristics and census period. Our results showed that game management was associated, albeit weakly, with higher abundance of raptors and ground-nesting birds, but no relationship (either positive or negative) was found for other guilds and species. Habitat was generally the most important factor explaining bird species richness and abundance. Our results suggest possibilities for promoting management systems that could maximize both hunting sustainability and conservation value of managed areas, particularly when management helps to improve or maintain beneficial habitats or practices for farmland birds., This study was funded by the Portuguese Foundation for Science and Technology (FCT) (PRAXIS/C/AGR/11062/1998) and by the European Commission under the 7th Framework Programme for Research and Technological Development through the project HUNT(212160, FP7- ENV-2007-1), and contributes to the project CGL2009-13029 from the Spanish Ministry of Science.

The Mediterranean Basin is a global hotspot of biodiversity. Hotspots are said to be experiencing a major loss of habitat, but an added risk could be the decline of some species having a special role in ecological relationships of the system. We reviewed the role of European rabbits (Oryctolagus cuniculus) as a keystone species in the Iberian Peninsula portion of the Mediterranean hotspot. Rabbits conspicuously alter plant species composition and vegetation structure through grazing and seed dispersal, which creates open areas and preserves plant species diversity. Moreover, rabbit latrines have a demonstrable effect on soil fertility and plant growth and provide new feeding resources for many invertebrate species. Rabbit burrows provide nest sites and shelter for vertebrates and invertebrates. In addition, rabbits serve as prey for a number of predators, including the critically endangered Iberian lynx (Lynx pardinus) and Spanish Imperial Eagle (Aquila adalberti). Thus, the Mediterranean ecosystem of the Iberian Peninsula should be termed “the rabbit’s ecosystem.” To our knowledge, this is the first empirical support for existence of a multifunctional keystone species in a global hotspot of biodiversity. Rabbit populations have declined drastically on the Iberian Peninsula, with potential cascading effects and serious ecological and economic consequences. From this perspective, rabbit recovery is one of the biggest challenges for conservation of the Mediterranean Basin hotspot., [ES] La Cuenca del Mediterra´neo es un a´rea de importancia para la biodiversidad global. estas a´reas de importancia esta´n experimentando una gran p´erdida de ha´bitat, pero el declive de algunas especies que juegan un papel especial en las relaciones ecolo´gicas del sistema pudiera ser un riesgo adicional. Revisamos el papel del conejo europeo (Oryctolagus cuniculus) como especie clave en la porcio´n del a´rea de importancia Mediterra´neo en la Pen´ınsula Ib´erica. Los conejos conspicuamente alteran la composicio´n de especies de plantas y la estructura de la vegetacio´n por medio del pastoreo y la dispersio´n de semillas, lo cual crea a´reas abiertas y preserva la diversidad especies de plantas. Ma´s aun, las letrinas de conejos tienen un efecto demostrable sobre la fertilidad del suelo y el crecimiento de plantas y proporcionan nuevos recursos alimenticios para muchas especies de invertebrados. Las madrigueras de los conejos proporcionan espacio para la nidificacio´n y refugio a vertebrados e invertebrados. Tambi´en, los conejos sirven como presa para un nu´ mero de depredadores, incluyendo al lince ib´erico (Lynx pardinus) en peligro cr´ıtico y al a´guila imperial (Aquila adalberti). Por lo tanto, el ecosistema Mediterra´neo de la Pen´ınsula Ib´erica deber´ıa ser denominado el “ecosistema de los conejos.” Hasta donde sabemos, este es la primera evidencia emp´ırica de la existencia de una especie clave multifuncional en un a´rea de importancia para la biodiversidad global. Las poblaciones de conejos han declinado dra´sticamente en la Pen´ınsula Ib´erica, con potenciales efectos en cascada y serias consecuencias ecolo´gicas y econo´micas. Desde esta perspectiva, la recuperacio´n de conejos es uno de los mayores retos para la conservacio´n del a´rea de importancia de la Cuenca del Mediterra´neo

Recently, a new method for differentiating juvenile and adult rabbits based on faecal pellet size was published. According to this method, pellets >6 mm diameter are inferred to be deposited by adults, while those 6 mm diameter in the same proportion as those 6 mm, whereas others deposit mostly pellets, M. Delibes-Mateos and C. Rouco were supported by a postdoctoral grant funded by the regional government of Castilla la Mancha (JCCM). Funding was provided by the projects FAU2006-00014-C02-02, PAI06-0170, and CGL2005-02340/BOS.

Letter., M. DELIBES-MATEOS is supported by a JAE-doc contract (Programa Junta para la Ampliación de Estudios), funded by CSIC and the European Social Fund.

Th e Cantabrian brown bear Ursus arctos population can be seen as a paradigm in conservation biology due to its endangerment status and genetic uniqueness. Th erefore, the need to obtain basic demographic data to inform management actions for conservation is imperative. Despite this, empirical data on the size and trends of the Cantabrian bear population are scarce. Here we present the fi rst estimates of population size (N c ) and eff ective population size (N e ) of the whole Cantabrian brown bear population. We genotyped 270 non-invasive samples collected during 2006 throughout the entire range of the population and subsequently identifi ed 130 individuals. Diff erent model estimators of N c based on capture – mark – recapture (CMR) procedures were compared. Th e average for the best three models (Mh Chao, Mh Darroch and CAPWIRE TIRM) yielded a total estimate of N c 223 individuals (CI 95% 183 – 278) and N e 50 (CI 95% 36 – 75) providing an N e / N c ratio of 0.22. Estimates for the two subpopulations commonly recognized in the Cantabrian range were N c 203 (CI 95% 168 – 260) and N e 47 (CI 95% 36 – 70) for the western subpopulation and N c 19 (CI 95% 12 – 40) and N e 9 (CI 95% 8 – 12) for the eastern subpopulation. Th ese data suggest that the Cantabrian brown bear population has increased recently, mainly in the western subpopulation, after a long period of decline and isolation which lead to the split of the population at the beginning of the 20th century. Population sizes in the early 1990s were thought to be only 60 individuals for the western subpopulation and 14 individuals in the eastern one. Th e eff orts to improve conservation policies made since then have probably contributed, to some extent, to the population increase during the last couple of decades.

The release of farm-reared game is considered a conflictive management tool because of its potential harmful ecological effects on wildlife and associated habitats. Estimates of the number of animals released and the area where this game management takes place could give an idea of the magnitude of their potential impacts. We aimed to quantitatively assess the release of farm-reared red-legged partridges (Alectoris rufa) in the province of Ciudad Real, central Spain, one of the main hunting areas in this country. We analysed the official permits that the regional government granted to the hunting estates to release farm-reared partridges between 2006 and 2012. Our results show that an average of 800,000 partridges were released annually in the study area. A decrease in the number of hunting estates that used this game management practice, as well as in the number of red-legged partridges officially released, was observed during the last years within the study period. This might be a consequence of the current economic crisis, rather than a change in management styles. Release intensity was spatially heterogeneous, being higher in the southeast of the study area, where historically red-legged partridge was more abundant. Most of the red-legged partridges released came from only four farms placed in northern Spain. Our results suggest that this management activity should be better monitored and that spatial regulations should also be considered., Work was supported by the European Commission (7th Framework Programme for R&D through project HUNT, 212160, FP7-ENV-2007-1) and by CSIC (PIE 201330E105).

Management goals in protected areas and/or communities usually include diversity as one of the most valuable and confident criteria. Nevertheless, the use of diversity and related indices as a means of evaluating successful management practices could produce conflicting results. Here we report a case study in one of the most important European protected areas. After 6 years of intensive conservation management of the Don~ana National Park, the general abundance and numbers of the target single-species conservation plan (the Iberian lynx) increased, although carnivore community diversity and evenness decreased. This was a result of a disproportionate increase of an oportunistic native species, the red fox. We propose the combined use of diversity, richness and evenness indices when monitoring management practices such as those reported here.

Mascotas que se establecen en la naturaleza: cerdos vietnamitas que viven en libertad en España Los cerdos vietnamitas (Sus scrofa) son una mascota habitual en Norteamérica y Europa; sin embargo, su popularidad ha disminuido recientemente y esto ha provocado que se abandonen cada vez más. El objetivo principal de este trabajo es identificar casos de cerdos vietnamitas que viven en libertad en España a través de una búsqueda exhaustiva en Google. Se han identificado 42 casos de cerdos vietnamitas que viven en libertad distribuidos por todo el país. El número de casos aumenta cada año, aunque la abundancia de la especie aún parece ser baja. También se han observado indicios de que los cerdos vietnamitas se reproducen en libertad y de posibles híbridos de éstos con jabalíes o cerdos asilvestrados. El hecho de que los cerdos vietnamitas vivan en libertad podría reducir el patrimonio genético de la población española de jabalí, así como agravar los daños que este ya causa en España (como los daños a cultivos o los accidentes de tráfico). Con vistas a evitar que se establezcan en hábitats naturales, es urgente evaluar y gestionar debidamente las observaciones de cerdos vietnamitas salvajes., Vietnamese potbellied (VPB) pigs (Sus scrofa) are a common pet in North America and Europe, but their recent decrease in popularity has increased their abandonment. Our main aim was to identify potential cases of free–living VPB pigs in Spain through an in–depth Google search. We identified 42 cases of free–living VPB pigs distributed throughout the country. The number of free–living VPB pigs reported increased by year but the species abundance still seems to be low. Signs of VPB pig reproduction and possible hybrids between VPB pigs and wild boar or feral pigs have been also reported. Free–living VPB pigs could erode the gene pool of the Spanish wild boar population and exacerbate the damage (e.g. crop damage or spread of diseases) already caused by wild board. Urgent evaluation and adequate management of wild VPB pig sightings is needed to prevent their establishment in natural habitats.

Hypothetical low-quality habitats can hold an overlooked conservation value. Some frugivorous mam- mals such as the red fox (Vulpes vulpes) and the European rabbit (Oryctolagus cuniculus) disperse many viable seeds of fleshy-fruited shrubs along the verges of soft linear developments (SLD), such as trails and firebreaks. However, seed arrival does not guarantee plant recruitment, since several post-dispersal processes can alter seed rain. To examine whether SLD verges assist shrub recruitment and establish- ment, we compared the density and the structure of a community of Mediterranean shrubs between SLD verges and the adjacent scrubland. Both seedlings and adult fleshy-fruited shrubs dispersed by foxes and rabbits reached higher densities along SLD verges than in the scrubland, suggesting SLD verges can be suitable habitats for shrub recruit- ment and establishment. Bird-dispersed shrubs showed a similar pattern, whereas shrubs dispersed by ungulates and badgers (Meles meles) as well as rockroses (Cistaceae) showed similar densities in both hab- itats. Shrub species composition and diversity were similar between habitats. Due to a marked differential seed arrival, SLD verges housed higher densities of fleshy-fruited shrubs than the adjacent scrubland. Established shrubs may attract seed-dispersing wildlife, and create proper environments for plant recruitment, generating a reforestation feedback. Incipient shrub populations along roadsides may act as stepping stones with potential to connect isolated populations in fragmented landscapes, where SLD are pervasive. We recommend careful management of frugivore populations and SLD verges in order to favor the diversity and the structural complexity of native vegetation while preventing the spread of invasive species.

The European rabbit is a growing problem for agriculture in parts of its natural range. In this study, our aim was to use historical records over two periods within the last 50 years to analyze trends in the number of requests made for rabbit control in Central Spain. We gathered data on rabbit control applications made in 1967 from Rabbit and Hare Control Authorization Records (CARs) and corresponding information for 2005 from Technical Hunting Plans (THPs). THPs are currently the official mechanism to apply for rabbit control licenses in the country. We show that although only 4.2 % of municipalities requested to control rabbits in 1967, this proportion was 71 % in 2005. Given that there is no evidence of rabbit population increases in the study region, we suggest that other factors may explain the observed rise in control requests. We contend that sport hunting is the main reason for the higher numbers of control requests in 2005. Evidence for this is the fact that hunting has increased since the 1960s as a means of augmenting income for landowners, and that the most requested method for control was the shotgun. Based on these results, we recommend that in order to adequately assess the real negative impacts of rabbits on human interests in Spain there is a need to implement a more robust data-gathering mechanism when control requests are made. We suggest the development of a more detailed control application form, similar to that used in Spain in the 1960s, in which the reason(s) for a rabbit control request can be clearly described., CAR-S was supported by a doctoral grant from the National Council on Science and Technology of Mexico (CONACyT). MD-M holds a Juan de la Cierva research contract (Ministerio de Ciencia e Innovación; European Social Fund). Funding was provided by projects PREG-05-22, CGL2009-11665/BOS, PEII 09-0097-4363, and POII09-0099-2557; all attract EUFEDER funds.

The knowledge of the historical range of organisms is necessary to understand distribution dynamics and their drivers as well as to set reference conditions and conservation goals. We reviewed written sources documenting the presence of lynxes in Spain between the sixteenth and nineteenth centuries, trying to infer whether Lynx records referred to the Iberian (Lynx pardinus) or the Eurasian (Lynx lynx) species. We compiled 151 spatially specific, non-redundant Lynx records, dating between 1572 and 1897. Records appeared split in two neatly separated areas, North and South. Arguably, all historical records from the South area correspond to Iberian lynx, since almost all of them fall within the estimated range of the species in the 1950s. This suggests that the core distribution area of the species remained relatively stable for centuries, until the second half of the twentieth century. The reported range in the 1950s is thus an appropriate scenario to set conservation goals for this critically endangered species. The North area extended from Galicia and northern Portugal to the Mediterranean, mainly occupying the Atlantic-climate area of northern Iberia. Reported direct measurements and the accumulation of indirect evidences suggest that most, if not all, northern Lynx records refer to the Eurasian lynx, which had been previously detected in archaeological sites of the area. This species was apparently present in the Iberian Peninsula until the early-nineteenth century. Our results provide an objective, baseline distribution of Lynx species in Spain useful to guide conservation efforts both at the Iberian and European levels.

Top predators have often been persecuted because of their supposed negative effects on species of economic concern on which they feed. In some cases, however, they may actually benefit their prey through intraguild predation on other smaller predators that share the prey. In each of two representative situations, in one of which lynx were present and in the other absent, we (1) estimated gross number of rabbits taken by lynx and Egyptian mongooses (smaller predators that are themselves preyed upon by lynx; (2) simulated size-structured rabbit populations of different densities, taking into account the reproductive value of the individuals taken by predators; and (3) estimated actual rabbit densities. Numbers of rabbits taken by predators during a year were found to be between 4.8 and 9.5 times greater when lynx were not present. After a year, rabbit population growth for an initial rabbit density of 15/ha was between 12% and 22% lower when lynx were not present. For lower initial rabbit densities, the positive effect of lynx presence on rabbits was greater. Actual rabbit densities in the areas used by lynx were 2–4 times higher than in areas not used by lynx, even though these areas were similar or identical in habitat composition. These results support the suggestion that removal of top predators may sometimes have a negative effect on prey populations of human economic concern.

An important goal of conservation biology is the maintenance of ecosystem processes. Incorporating quantitative measurements of ecosystem functions into conservation practice is important given that it provides not only proxies for biodiversity patterns, but also new tools and criteria for management. In the satellite era, the translation of spectral information into ecosystem functional variables expands and complements the more traditional use of satellite imagery in conservation biology. Remote sensing scientists have generated accurate techniques to quantify ecosystem processes and properties of key importance for conservation planning such as primary production, ecosystem carbon gains, surface temperature, albedo, evapotranspiration, and precipitation use efficiency; however, these techniques are still unfamiliar to conservation biologists. In this article, we identify specific fields where a remotely-sensed characterization of ecosystem functioning may aid conservation science and practice. Such fields include the management and monitoring of species and populations of conservation concern; the assessment of ecosystem representativeness and singularity; the use of protected areas as reference sites to assess global change effects; the implementation of monitoring and warning systems to guide adaptive management; the direct evaluation of supporting ecosystem services; and the planning and monitoring of ecological restorations. The approaches presented here illustrate feasible ways to incorporate the ecosystem functioning dimension into conservation through the use of satellite-derived information. © 2012 Springer Science+Business Media B.V.