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Background: While today different drugs have been used to treat cancer, none of them have proved to be effective. Heavy metal complexes (e.g., platinum and palladium) can be useful in the treatment of cancer. The purpose of this study was to evaluate the effects of newly synthetized palladium complexes on the morphology and clonogenicity of liver cancer cell line (HepG2). Materials and Methods: New palladium complexes were synthesized in the chemistry lab, University of Sistan and Baluchesta. The cytotoxicity and anti-cancer effects of newly synthesized complexes were evaluated using the clonogenic and cell death assay (apoptosis and necrosis) using acridine orange/ ethidium bromide double staining. Results: The results of this study showed that the new complexes had potential concentration-dependent effects on HepG2 cell line. There was a significant difference between the number of colonies formed after treatment with different concentrations of palladium complexes (0.5, 1, 2, 4 and 8 μg/ml) compared to those in the control group (P<0.01). Furthermore, the cells stained with acridine orange/ethidium bromide indicated the occurrence of apoptosis in cancer cells after the treatment with various concentrations of palladium complexes. Conclusion: The results of this study show the anti-cancer effect of palladium complexes on HepG2 cell lines. Also, the palladium complexes can reduce the number of cell colonies and cause cell death by apoptosis. [ABSTRACT FROM AUTHOR]

Introduction: Chemotherapy drugs are used to treat various types of neoplasms. In dong so, such drugs leave many side effects. Furthermore, in recent years, several attempts have been made to develop drugs based on platinum or palladium which have low toxicity and is more sensitive to the drug-resistant diseases. Objective: To evaluate the effects of newly synthetized palladium complexes, as anticancer drugs, on gastric cancer cell line (AGS). Materials & Methods: In this study, gastric cancer cell line (AGS) was purchased from Pasteur Institute, Iran and cultivated in RPMI 1640 and then, the cytotoxic effects of various concentrations of newly synthesized complexes were evaluated by clonogenic assay and acridine orange/ethedium bromide staining. Results: The results showed that the new complexes have different effects in concentration-dependent manner so that complexe l > 0.1 .g /ml, complexe2 > 0.05 .g /ml and complexe 3 > 1 .g /ml lead to cell death by apoptosis and necrosis. Comparison of the number of colonies formed after treatment with concentrations of palladium complexes showed significant differences, compared with controls (P<0.001). Conclusion: In this study, it was demonstrated that the use of low concentrations of palladium complexes of dithiocarbamate derivative increases apoptosis and necrosis, also, reduces the number of cell colonies, thus, it can be considered as an alternative drug for the treatment of gastric cancer. [ABSTRACT FROM AUTHOR]